Cardiac fibroblasts
Original Article DIM attenuates TGF-β1-induced myofibroblast differentiation in neonatal rat cardiac fibroblasts
... The total proteins were extracted from neona- tal rat cardiac fibroblasts, the protein concen- trations were determined with the BCA protein assay reagent (Thermo, 23225). Proteins sam- ples (20 μg) in a ...
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View from the heart: cardiac fibroblasts in development, scarring and regeneration
... understanding cardiac fibroblasts and fibrosis over the last couple of years, many questions remain ...the cardiac fibroblast in various disease settings, including congenital malformations and heart ...
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Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload
... both cardiac hypertrophy and heart ...being fibroblasts (4, ...collagens), cardiac fibroblasts produce a vari- ety of growth factors that likely mediate an interplay between car- diac ...
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Estrogen modulates the influence of cardiac inflammatory cells on function of cardiac fibroblasts
... Total protein was extracted from the cultured cardiac fibroblasts by homogenization of the cells with buffer containing a protease inhibitor cocktail (Pierce, Rockford, IL, USA). Protein concentrations were ...
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Osteopontin is produced by rat cardiac fibroblasts and mediates A(II) induced DNA synthesis and collagen gel contraction
... in cardiac injury models associated with inflammation in the rat (9) and hamster ...rat cardiac fibroblasts, generally all cells immunostained posi- tively for ...whether cardiac ...
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Neferine inhibits proliferation and collagen synthesis induced by high glucose in cardiac fibroblasts and reduces cardiac fibrosis in diabetic mice
... on cardiac fibrosis induced by diabetes mellitus, as well as the underlying molecular ...and cardiac function was assessed using ...vitro, cardiac fibroblasts were cultured in normal or ...
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Cardiac fibroblasts in pressure overload hypertrophy: the enemy within?
... hypertrophy, cardiac function decreased only slightly, without signs of cardio- myocyte ...in cardiac fibroblasts associated with irreversible maladaptive remodeling may represent the most ...
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Transforming growth factor β1 induces differentiation of rainbow trout (Oncorhynchus mykiss) cardiac fibroblasts into myofibroblasts
... pre-existing fibroblasts in response to transforming growth factor- β 1 (TGF- β ...trout cardiac fibroblasts with human TGF- β 1 causes an increase in collagen ...trout cardiac ...
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Proapoptotic Rassf1A/Mst1 signaling in cardiac fibroblasts is protective against pressure overload in mice
... represses cardiac fibroblast proliferation. (A–G) Fibroblasts were isolated from neonatal rat hearts and passaged a minimum of 3 times prior to ...(C) Fibroblasts were treated with H 2 O 2 (100 μM) ...
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The role of integrins in mechano-transduction leading to enhanced collagen synthesis in human cardiac fibroblasts
... cell layers with various antibodies to cytoskeletal and surface proteins. At confluence cells were trypsinised as described above and plated into 8-well chamber slides at a density of 10, 000 cells per well in 10 % ...
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CaMKIIδ interacts directly with IKKβ and modulates NF-kB signalling in adult cardiac fibroblasts
... CaMKII inhibition. (A(i)) A typical population of adult rat cardiac fibroblasts (p1) in culture (x20 magnification). (B) CFs were double-stained for (i) vimentin/DAPI (ii) von-Willebrand factor(vWF)/DAPI ...
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Functional Alterations of Ion Channels From Cardiac Fibroblasts in Heart Diseases
... dysfunction. Cardiac fibroblasts (CF), responsible for the maintenance of extracellular matrix and fibrosis process, play an important role in cardiac health and ...
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The role of cardiac fibroblasts in the transition from inflammation to fibrosis following myocardial infarction
... 2005). Cardiac fibroblasts (CF) are the most prevalent resident myocardial cell type, accounting for up to two-third of heart cells (Porter and Turner, ...
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Original Article Berberine regulates proliferation, collagen synthesis and cytokine secretion of cardiac fibroblasts via AMPK-mTOR-p70S6K signaling pathway
... in cardiac fibroblasts treated with or without berberine (green: α-SMA; blue: ...of cardiac fibroblasts stimulated with Ang II was inhibited by ...in cardiac fibroblasts of the ...
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Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells
... minutes. Cardiac fibroblasts were transfected overnight as described ...eGFP+ cardiac fibroblasts using Incyte software (eMD Millipore, Billerica, Ma, ...
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Inhibition of autophagy inhibits the conversion of cardiac fibroblasts to cardiac myofibroblasts
... concomitant cardiac fibrosis is very high in developed ...to cardiac fibrosis as they convert to hypersynthetic cardiac ...reduced cardiac function and ultimately heart ...atrial ...
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Trypanosoma cruzi activates mouse cardiac fibroblasts in vitro leading to fibroblast myofibroblast transition and increase in expression of extracellular matrix proteins
... Since the activation of myofibroblasts is the initial stage on the fibrogenesis in injured tissues [10], we questioned whether T. cruzi infection would activate the differenti- ation of CFs into myofibroblasts. This ...
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Exercise promotes a cardioprotective gene program in resident cardiac fibroblasts
... to cardiac homeostasis in healthy individuals has been largely ...develop cardiac fibrosis. We found that MTs secreted from fibroblasts are taken up by CMs in vitro and reduce the damag- ing effects ...
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Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction
... in cardiac fibrosis development by inhibiting other target genes ...that cardiac stress increased miR21 expression, leading to SPRY1 suppression and the followed activation of ERK-MAPK, which played a ...
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Hypoxia-induced H19/YB-1 cascade modulates cardiac remodeling after infarction
... To elucidate the mechanism of lncRNA H19 in affecting the changes in fibrosis, we performed chromatin isolation by RNA purification (ChIRP) in NIH3T3 cells to capture RNA binding proteins that interact with lncRNA H19 ...
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