Cartilage Explants
Standardized butanol fraction of WIN 34B suppresses cartilage destruction via inhibited production of matrix metalloproteinase and inflammatory mediator in osteoarthritis human cartilage explants culture and chondrocytes
... in cartilage explants at 7 days from the onset of culture was incubated in the presence of 1% w/v bovine serum albumin (BSA) in phosphate buf- fered saline (PBS)/Tween 20 ...
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Prostaglandin E2 synthesis in cartilage explants under compression: mPGES 1 is a mechanosensitive gene
... costal cartilage explants subjected to compres- sive stress for 18 hours were fixed in ethanol, embedded in methyl methacrylate and cut into serial sections that were immunostained with antibody against ...
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A Spectrophotometric Analysis of Human Osteoarthritic Cartilage Explants Subjected to Specific Capacitively Coupled Electric Fields
... articular cartilage explants resulted in up-regulation of cartilage matrix gene expression and down-regulation of metalloproteinase gene ...modifying cartilage structure, freshly har- vested ...
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Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro
... cultured cartilage explants and found that these not only did penetrate and react with CII, but also had disruptive effects on a pre-established cartilage ...the cartilage matrix we used ...
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Suppression of MMP activity in bovine cartilage explants cultures has little if any effect on the release of aggrecanase derived aggrecan fragments
... from cartilage explants stimulated with catabolic ...articular cartilage explants were stimulated with catabolic cytokines OSM and TNFα in the absence (open circles) and presence (closed ...
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Sprifermin (rhFGF18) modulates extracellular matrix turnover in cartilage explants ex vivo
... using cartilage explants retrieved from three inde- pendent cows, one cow per ...the explants were cultured for 3 weeks with stimulation by sprifermin (900 ng/mL) or placebo in one media change per ...
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The metabolic dynamics of cartilage explants over a long term culture period
... the explants studied at day 0 experienced more cell death than the explants that were given sufficient time to ...These cartilage explants were all washed once with 70% ethanol, which might ...
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Na+, K+ ATPase subunit composition in a human chondrocyte cell line; evidence for the presence of α1, α3, β1, β2 and β3 isoforms
... bovine cartilage, the density of Na + , K + -ATPase correlates with matrix glycosaminoglycan concentrations and hence with tissue Na + [27] suggesting a feedback mechanism for the regulation of Na + , K + -ATPase ...
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Inhibition of hyaluronan export reduces collagen degradation in interleukin 1 treated cartilage
... osteoarthritic cartilage allowed the diffusion of ...activated cartilage. Cartilage explants were incubated in the absence and presence of IL-1α and vardenafil, and enzymes released from the ...
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Differential recovery of glycosaminoglycan after IL 1 induced degradation of bovine articular cartilage depends on degree of degradation
... of cartilage to repair osteoarthritis-like ...bovine cartilage explants recovering from trypsin- induced GAG depletion ...IL-1-degraded cartilage, our data are consis- tent with the temporal ...
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Original Article Cordycepin modulates inflammatory and catabolic gene expression in interleukin-1beta-induced human chondrocytes from advanced-stage osteoarthritis: an in vitro study
... culturing cartilage explants and human articu- lar chondrocytes in ...stimulated cartilage explants cell viability on IL-1β-stimulated ...
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Cyclooxygenase inhibition lowers prostaglandin E2 release from articular cartilage and reduces apoptosis but not proteoglycan degradation following an impact load in vitro
... on cartilage: increasing proteoglycan and collagen synthesis [41], stimulating proliferation and aggrecan synthesis [42], up- regulating glucocorticoid receptors [43] and, at low concen- trations, stimulating ...
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Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor alpha and oncostatin M
... OSM-treated cartilage explants caused a slight deple- tion of S-GAGs (an amount harmonious with the level of uronic acid remaining in the ...the cartilage ex- ...the cartilage explant model ...
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Arthropod steroid hormone (20 Hydroxyecdysone) suppresses IL 1β induced catabolic gene expression in cartilage
... mouse cartilage, whereas aggrecanases are expressed in the superficial cartilage ...osteoarthritic cartilage damage and is followed by the irreversible loss of collagens ...degenerative ...
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Comparative analysis of sodium coupled vitamin C transporter 2 in human osteoarthritis grade 1 and grade 3 tissues
... osteoarthritic cartilage explants tissues, these tissues were stained and characterized using Safranin O and Fast Green or Hematoxylin and Eosin (Additional file 1: Figure ...
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Targeted designed variants of alpha 2 macroglobulin (A2M) attenuate cartilage degeneration in a rat model of osteoarthritis induced by anterior cruciate ligament transection
... articular cartilage explants (BCE) to screen targeted designed A2M variants in vitro, fluores- cence molecular tomography (FMT), a new advanced method for measuring certain catabolic protease levels in ...
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Peripheral blood derived mononuclear cells enhance osteoarthritic human chondrocyte migration
... adult cartilage do not migrate due to the surrounding highly tensile colla- gen network resulting in a highly pressurized matrix [4], essentially trapping the cells within the ...in cartilage ...
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Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants
... the cartilage tissue was sliced at a thickness of 1 ...groups. Cartilage explants were equilibrated for 2 days in culture medium (250 μl of high-glucose DMEM with supplements (as above) per explant ...
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Impairment of glyoxalase 1, an advanced glycation end product detoxifying enzyme, induced by inflammation in age related osteoarthritis
... To better establish the relationship between inflamma- tion/oxidative stress and CML accumulation in vivo, we should have been able to demonstrate the presence of an inflammatory activity (such as IL-1 or IL-6) in the OA ...
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Activation of proteinase activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proinflammatory pathways capable of inducing cartilage degradation: a basic science study
... OA cartilage, one of the most important serine protease systems is the plasminogen activator (PA) plasmin, in which the urokinase PA (uPA) plays a major role ...on cartilage macromolecules, has been shown ...
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