CAS systems
On the Origin of Reverse Transcriptase Using CRISPR Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires
... Phylogeny of reverse transcriptases associated with CRISPR-Cas systems. Pre- vious phylogenetic analysis of the RT superfamily has suggested that the CRISPR- associated RTs could be derived from a single ...
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CRISPR-Cas Systems and Genetic Organization in Lactic Acid Bacteria.
... CRISPR-Cas systems (Chapter ...whether systems are likely active or not from solely an in silico ...these systems, but performing systematic searches will be interesting for studying microbial ...
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Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems
... these systems contain two universal genes: cas1, a metal-dependent DNAse with no sequence specificity that could be involved in the integration of the alien DNA (spacer) into CRISPR cassettes [21,22], and cas2, a ...
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CRISPR-Cas Systems in Lactic Acid Bacteria.
... CRISPR- Cas systems in the genus Bifidobacterium (77%), compared to the estimated prevalence in bacteria (45%, 1176/2612, as of the August 2014 update) (25) (Table ...I, systems and six Type II ...
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CRISPR-Cas Systems for Diagnosing Infectious Diseases
... CRISPR-Cas systems, including pros and cons for developing molecular diagnostic tests and applications of each type to detect a wide range of infectious ...Many Cas proteins (Cas9, Cas12, Cas13, ...
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Discovering, Characterizing, and Applying CRISPR-Cas Systems in Bacteria.
... CRISPR-Cas systems can be ...these systems exist on ~50% of sequenced bacteria 1,2 , although recent results suggest this number actually may be much lower 3 ...these systems provide a unique ...
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Understanding and Exploiting the Properties of Type I CRISPR-Cas Systems
... Despite the predominant focus on genome editing, CRISPR-Cas systems are also revolutionizing the programmable regulation of gene expression. This feat was first demonstrated with CRISPR-Cas9 in two landmark ...
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Systematic evaluation of CRISPR Cas systems reveals design principles for genome editing in human cells
... The ideal CRISPR-Cas system is one with both high cleavage efficiency and high target specificity. Two re- cent studies reported that AsCpf1 and LbCpf1 appeared to satisfy both these criteria [19, 20], suggesting ...
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Presence of Type I F CRISPR/Cas systems is associated with antimicrobial susceptibility in Escherichia coli
... Microsoft Word Aydin et al unformatted docx The presence of Type I F CRISPR/Cas systems is associated 1 with antimicrobial susceptibility in Escherichia coli 2 Seyid AYDIN1, Yoann PERSONNE1, Enas NEWI[.] ...
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Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins
... CRISPR-Cas systems have evolved to select short sequences (protospacers) of the invading DNA and inte- grate them as spacer sequences into CRISPR loci to provide sequence-specific immunity [5, ...immune ...
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Programmable Removal of Bacterial Strains by Use of Genome Targeting CRISPR Cas Systems
... CRISPR- Cas systems being exploited to control bacterial populations in diverse ecological niches and scientific ...CRISPR-Cas systems could be used to selectively clear contami- nating ...
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Phylogenetic Distribution of CRISPR Cas Systems in Antibiotic Resistant Pseudomonas aeruginosa
... CRISPR-Cas systems have historically been FIG 5 Correlations between CRISPR-Cas subtypes and antibiotic ...CRISPR-Cas systems have, on average, smaller genomes than those lacking the ...
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The Pseudomonas aeruginosa accessory genome elements influence virulence towards Caenorhabditis elegans
... CRISPR-Cas systems. Notably, the presence of active CRISPR-Cas systems (by the criterion of absence of anti- CRISPR genes) also statistically correlates with increased virulence ...
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Campylobacter jejuni acquire new host derived CRISPR spacers when in association with bacteriophages harboring a CRISPR like Cas4 protein
... CRISPR-Cas systems are generally reported to bind and cleave DNA through the action of Cas9 (Jinek et ...in Cas-interference depen- dent modulation of swarming behavior and biofilm formation that is ...
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Evolution of RNA- and DNA-guided antivirus defense systems in prokaryotes and eukaryotes: common ancestry vs convergence
... regulatory systems. All cellular life forms possess defense systems against viruses and mobile genetic elements, and in most of them some of the defense mechanisms involve small guide RNAs or DNAs that ...
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On the global CRISPR array behavior in class I systems
... these systems qualitatively and quantita- ...Cas systems. In particular, it has been determined that these systems can be classified into two classes, based on the types and sequences of ...
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Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein.
... CRISPR-Cas systems are generally reported to bind and cleave DNA through the action of Cas9 (Jinek et ...in Cas-interference depen- dent modulation of swarming behavior and biofilm formation that is ...
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Functional Genomic Studies of Lactic Acid Bacteria.
... specific cas genes and CRISPR-array components were elucidated ...CRISPR-Cas systems, two of them (CRISPR1 and CRISPR3) are classified as Type II-A systems that are innately active in both ...
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Diversity of CRISPR Cas immune systems and molecular machines
... CRISPR-Cas systems and adaptive immunity The characterization of biological processes that under- lie CRISPR-based adaptive immunity in bacteria and ar- chaea has shaped many crucial aspects of the past ...
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CRISPR Cas Biology and Its Application to Infectious Diseases
... CRISPR-Cas systems have now come to light, with rapidly diversifying applications of CRISPR ...CRISPR-Cas systems, high- light key features of CRISPR-Cas9 technology, and summarize existing ...
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