Cas9/CRISPR
Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9
... ing to the regions directly surrounding the sgUPRT target se- quence (Fig. 2A; also, see Tables S1 and S2). Following cotransfec- tion, parasites were selected with pyrimethamine, and the stably resistant pool was ...
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CRISPR/Cas9 mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes
... of CRISPR/Cas9 genome edit- ing to gut-associated bacteria of mosquitoes has significant applied ...the CRISPR/Cas9 system to integrate genes in intergenic regions of the bacterial chromosome ...
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Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... contains Cas9/sgRNA cassettes with targeting sgRNA protospacer and restriction sites for cloning DNA templates, allowing construction of a single vector for vari- ous gene modification ...the Cas9/sgRNA ...
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Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
... the CRISPR/Cas9 tool in ...the CRISPR/Cas9 system requires the controlled expres- sion of cas9 and the constitutive expression of sgRNA in the presence of DNA editing ...of ...
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Application of CRISPR/Cas9 for biomedical discoveries
... Cas: CRISPR-associated proteins; Cas9: Native Cas9 nuclease; Cas9n: Cas9 nickase (containing only one functional catalytic domain); CIBN: Calcium and integrin-binding protein 1 N terminal ...
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CRISPR/Cas9 Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment
... After obtaining mutant-enriched mixed populations by FACS, clonal populations of epimastigotes with TcPFR2 disrupted were obtained by serial dilutions in 96-well plates as described in Ma- terials and Methods. To ...
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Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9
... Reverse genetics approaches have been important in dem- onstrating gene functions, genetic engineering, and un- derstanding complex biological processes. In the present study, we successfully established the ...
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In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
... The CRISPR/Cas system from Streptococcus pyogenes, which consists of Cas9 protein and two RNAs (CRISPR RNA [crRNA] and trans-activating crRNA [tracrRNA]), is most commonly used ...First, Cas9 ...
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Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 System
... by CRISPR – Cas9 mediated homologous ...The Cas9 cleavage site at the lab-1 locus (652 bp) is located 310 base downstream of the ATG (arrow, top ...
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CRISPR Cas9 Mediated Single Gene and Gene Family Disruption in Trypanosoma cruzi
... the CRISPR-Cas9 system for the genetic engineer- ing of ...the Cas9-induced double-stranded breaks (DSBs) in ...of CRISPR-Cas9-induced ...of CRISPR-Cas9 in ...that ...
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Targeted Editing of the StPDS Gene using the CRISPR/Cas9 system in Tetraploid Potato
... the CRISPR/Cas9 vector that contains an hCas9 protein may have caused lower editing efficiency; in addition, different crop species such as potato and rice often result in different efficiencies (Butler et ...
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Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR Cas9
... CRISPR4) contained the spacer with the fewest modified C resides on both DNA strands and therefore the lowest modification den- sity. Infection with T4(glc-HMC) was reduced 2- to 10-fold in repeated assays, and plaque ...
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CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum
... from Cas9-induced DNA ...the CRISPR/Cas9 system may be challenging to use in the context of the subtelomeric or even central var genes, and we recommend caution and genomic sequencing for any mutant ...
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CRISPR/Cas9 TECHNOLOGY A NEW BOON IN GENOME EDITING
... triggers Cas9 nuclease activity by activating the HNH and RuvC domains, supported by the observation of HNH domain flexibility within the Cas9-sgRNA-DNA ternary ...each Cas9 ortholog, even within the ...
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CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases
... with CRISPR/Cas9 is low, rap- idly developing technology has improved its knock-in ef- ...of Cas9 pro- tein complex with chemically synthesized dual RNA was reported to generate knock-in mice with up ...
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Review Article Status and contribution of CRISPR/Cas9 based gene-editing system in the development of a low-immunogenic wheat variety
... of CRISPR/Cas9 in developing celiac-safe wheat variety were searched using the keywords ‘celiac and CRISPR’, ‘CRISPR in celiac disease’, ‘Wheat engineering with CRISPR’, ‘Low ...
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CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major
... the CRISPR/Cas9 strategy used in ...recombinant Cas9 protein, we generated several rel null mutant alleles that were easily detectable by PCR screening and transmissible from generation to ...
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CRISPR Cas9 Mediated Genome Editing in Leishmania donovani
... by Cas9 in mam- malian DNA are mainly repaired by the indel-forming nonho- mologous end-joining (NHEJ) pathway, resulting in inactivation of the targeted ...by CRISPR-Cas9 in the LdMT-targeting ...
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How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising
... of CRISPR/Cas9 system is low needing to be ...the CRISPR/Cas9 therapy, potentially leading to rebound viremia and repopulation of HBV in the liver after the cessation of the antiviral ...
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CRISPR ethics & imagination : an evaluation of the role of DIY CRISPR kit in Crispr Cas9 deliberations
... the CRISPR enabled genome modification as a serious and revolutionary technology, and thereby would ignore its actual complexity and ...“Is CRISPR so easy that even amateurs like me can make meaningful ...
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