cas9/CRISPR genome editing
A CRISPR Assisted Nonhomologous End Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis
... including CRISPR–CRISPR-associated protein 9 (Cas9) and CRISPR- Cas12a (Cpf1), have been widely used as genome-editing tools (12, ...The CRISPR-Cas9 system ...
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CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum
... ABSTRACT Plasmodium falciparum relies on monoallelic expression of 1 of 60 var virulence genes for antigenic variation and host immune evasion. Each var gene contains a conserved intron which has been implicated in ...
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Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides
... increases editing efficiency (35, 43) while others find the opposite effect ...to Cas9 editing efficiency in ...of editing efficiency in this multiplexed sgRNA design, relative to the ...
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Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
... the genome is an important method for increasing gene expression, especially in the organism without episomal plasmids [28, ...study, editing templates for multi-copy integrations did not contain any ...
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Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes
... maintain genome integrity, the cell must repair generated ...in genome editing, an exogenous repair DNA (donor DNA) flanked by homologous sequences to both sides of the DSB is typically ...
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Applications of CRISPR-Cas9 mediated genome engineering
... in genome engineering based on the CRISPR-Cas9 system enables fast functional characterization of putative disease genes in various mouse models via somatic genome editing ...A ...
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Precision cancer mouse models through genome editing with CRISPR-Cas9
... Cancer genome sequencing studies have identified a variety of genetic al- ...the CRISPR-Cas9 system with well-studied conditional mouse models of ...atic genome engineering approach that ...
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Genome Editing of Babesia bovis Using the CRISPR/Cas9 System
... bovis genome, including several ex- panded gene families which are unique for Babesia ...the CRISPR/Cas9 system as a robust genetic engineering tool for ...
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CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies
... Abstract: CRISPR-Cas9 is revolutionizing the field of genome editing in non-model ...sequenced genome and amenable to germ line ...efficient genome editing tools to ...
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A RecET-assisted CRISPR–Cas9 genome editing in Corynebacterium glutamicum
... chromosome-borne Cas9 maintained at the relative lower level compared to plasmid-borne Cas9, which might be the reason for no impacts of chromosome-borne Cas9 expression on cell ...plasmid-borne ...
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Tissue specific genome editing in Ciona embryos by CRISPR/Cas9
... (B) CRISPR/Cas9-targeted mutagenesis of Ebf exon 9 (35 μ g Sox1/2/3>nls::Cas9::nls, 25 μ g ...a CRISPR/Cas9-resistant form of Ebf (45 μ g Ebf>Ebf m774 ), as Isl>YFP is now seen ...
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Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
... in CRISPR/Cas9 vectors [13, ...[12]. CRISPR/Cas9 vector systems using the U6 promoter to drive sgRNA expression have been successfully used in various plant species, with the OsU6a, OsU6b and ...
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Review Article Status and contribution of CRISPR/Cas9 based gene-editing system in the development of a low-immunogenic wheat variety
... the genome with the non-toxic genes in order to develop a non-toxic wheat ...of CRISPR/cas9 system to edit the wheat ...wheat genome and have shown promising results (Table 1) ...
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CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications
... precise genome editing, multiple round of marker selection and recycling are time consuming and some scars would be left in genome, which will bring genetic instability [17, ...and ...
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Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
... on CRISPR-directed nuclease activity offer the potential for maximally convenient engineering of target specificity to effect changes to the ...a Cas9 nuclease forms a complex with two RNAs, called the ...
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A redesigned CRISPR/Cas9 system for marker free genome editing in Plasmodium falciparum
... exhibited editing was dependent on the drug selection time as was observed in both the knock-out and knock-in ...marker-free CRISPR/Cas9 editing ...% editing efficiency) in both previ- ...
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Host Pathogen Wars: New Weapons from Biotechnology and Genomics
... draft genome sequence of crop plants and their pathogens, and the new gene editing tools such as TALENs and CRISPR-cas9 are offering addi- tional avenues for researchers to explore new ...
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Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
... survived CRISPR mediated DSBs by A-EJ and under- went large DNA ...by editing of lacZ ...precise editing as ...the Cas9 cleavage locus were designed and ...suggest CRISPR mediated DSBs ...
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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9 Mediated Counterselection
... and CRISPR/Cas9-mediated counterselection in ...and Cas9. Targeting of Cas9 to a locus of interest is achieved by cloning a 20-bp DNA oligonucleotide (protospacer element) into the sgRNA ...
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Development of a CRISPR-Cas9 System for Efficient Genome Editing of Candida lusitaniae
... and CRISPR-associated gene 9 (Cas9) system to enable genome editing of ...of CRISPR-Cas9 components under species-specific promoters is necessary for efficient gene targeting and ...
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