Catalytic core
Catalytic Core of Alphavirus Nonstructural Protein nsP4 Possesses Terminal Adenylyltransferase Activity
... polymerase catalytic core due to sequence similarities with other poly- merases, the presence of conserved polymerase motifs, second- ary structure predictions, and various temperature-sensitive mutations ...
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The Structure of an Archaeal Viral Integrase Reveals an Evolutionarily Conserved Catalytic Core yet Supports a Mechanism of DNA Cleavage in trans
... integrase catalytic core composed of three succes- sive alpha helices ( ␣ 1 to ␣ 3) followed by a three-stranded, up- and-down, antiparallel -sheet (1 to 3) and four additional helices ( ␣ 4 to ␣ 7) ...
5
A New Class of Allosteric HIV-1 Integrase Inhibitors Identified by Crystallographic Fragment Screening of the Catalytic Core Domain
... IN catalytic core domain (CCD) followed by a fragment expansion approach, we have identified thiophenecar- boxylic acid derivatives that bind at the CCD-CCD dimer inter- face at the principal lens ...
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Characterization of the HIV 1 integrase chromatin and LEDGF/p75 binding abilities by mutagenic analysis within the catalytic core domain of integrase
... As a functional component of PICs [5,6], the roles of LEDGF or p75 during lentiviral DNA integration have attracted increasing interest in recent years. LEDGF/p75, discovered as a general transcriptional co-activator ...
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Evidence for Posttranslational Protein Flavinylation in the Syphilis Spirochete Treponema pallidum: Structural and Biochemical Insights from the Catalytic Core of a Periplasmic Flavin Trafficking Protein
... FAD pyrophosphatase (EC 3.6.1.18) catalyzes the hydrolysis of FAD, forming AMP and FMN. To date, the Ftp (TP0796) of T. pallidum is the first bacterial FAD pyrophosphatase shown to have a strict requirement for Mg 2⫹ for ...
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Genetic Analyses of DNA-Binding Mutants in the Catalytic Core Domain of Human Immunodeficiency Virus Type 1 Integrase
... tation. Although three-dimensional structures of IN-DNA complexes have not been solved, models based on protein fragment structures (14, 71), photo-cross-linking (31, 41), and/or computer simulations (2, 18, 32, 58, 59, ...
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Spinal muscular atrophy treatment via targeting smn2 catalytic core
... [0029] Another aspect of the invention is directed to a method of enhancing the level of exon 7-containing SMN2 mRNA relative to exon-deleted SMN2 mRNA in a cell or cell.. calculated fro[r] ...
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Spinal muscular atrophy treatment via targeting smn2 catalytic core
... The compositions and methods of the instant invention include short oligonucleotide reagents e.g., oligoribonucleotides that effectively target sites in the SMN2 pre-mRNA, thereby modula[r] ...
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Human immunodeficiency virus integrase inhibitors efficiently suppress feline immunodeficiency virus replication in vitroand provide a rationale to redesign antiretroviral treatment for feline AIDS
... Amino acid sequence alignment of the lentiviral integrase catalytic core domain (IN CCD). Amino acid sequences were aligned with BioEdit and alignments manually edited to eliminate gaps. FIV-Fca, FIV-Oma, ...
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Structural insights into the regulation of PLC-[beta]2
... the core of PLC-β2 (residues 1 – 799) in complex with activated Rac1 (residues 1 – 189) bound to the non-hydrolyzable nucleotide analog, ...the catalytic core of PLC- δ, possessing ...
106
Lpg0393 of Legionella pneumophila Is a Guanine-Nucleotide Exchange Factor for Rab5, Rab21 and Rab22
... the catalytic core of Rabex-5 in complex with nucleotide-free Rab21 ...The catalytic core of Rabex-5 is composed of residues 139-391 that assemble into two domains: an N- terminal four-helix ...
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Raltegravir: molecular basis of its mechanism of action
... gaps, thereby completing the integration process [10]. The final product is a covalently inserted viral genome, colinear with cellular genes, with a short (e.g. 5 bp) du- plication on either side, the length of which is ...
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CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation
... The flexible head-to-head coordination in the absence of CYK4 is consistent with the previ- ous electron microscopic observation of dimeric ZEN-4 bound to microtubules [65], in which two motor domains in a single dimer ...
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Soluble expression, purification and characterization of the full length IS2 Transposase
... the catalytic domain of the DNA ...the catalytic domain of IRR, due to an additional specific or stochastic DNA contact by the substituted threonine, produced the subsequent ...the catalytic domain ...
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of kinetically active residues
... blue ribbon). Sofosbuvir is green molecule inside the HCV RdRp catalytic core. SAGNM predictions are dark yellow atoms. Sofosbuvir is green molecule inside the 455. catalytic core. The[r] ...
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The Est1 Subunit of Saccharomyces cerevisiae Telomerase Makes Multiple Contributions to Telomere Length Maintenance
... The telomerase-associated Est1 protein of Saccharomyces cerevisiae mediates enzyme access by bridging the interaction between the catalytic core of telomerase and the telomere-binding protein Cdc13. In ...
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Transcription Factor YY1 Interacts with Retroviral Integrases and Facilitates Integration of Moloney Murine Leukemia Virus cDNA into the Host Chromosomes
... FIG. 1. YY1 directly interacts with INs of MoMLV, ASV, and HIV-1. (A) Schematic drawing of the GST-IN of MoMLV and its deletion mutants. HHCC, zinc finger; DDE, catalytic center; a.a., amino acid. (B) Results of ...
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Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by α4 Protein in HEK293 Cells
... Interestingly, a fraction of PP2A/C was found to form a complex with the α4 protein, the homolog of Tap42 in yeast. The amount of Tap42 associated PP2A/C was predicted to be about 2% of the whole PP2A/C pool. However, ...
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Electrochemical insights into the mechanism of NiFe membrane-bound hydrogenases
... The bidirectional catalytic activity of NiFe hydrogenases is clearly revealed in cyclic voltammetry experiments such as that shown in Figure 4. The electrochemical potential of the hydrogenase-coated electrode is ...
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Draft genome sequence of Annulohypoxylon stygium, Aspergillus mulundensis, Berkeleyomyces basicola (syn. Thielaviopsis basicola), Ceratocystis smalleyi, two Cercospora beticola strains, Coleophoma cylindrospora, Fusarium fracticaudum, Phialophora cf. hyalina, and Morchella septimelata
... The genome of BP5796 was sequenced to 100-fold coverage, yielding 45 scaffolds with N50 of 2.0 Mb. The assembled genome size was 40.4 Mb, and a total of 13257 genes were predicted. The GC content of this genome is 48.5 ...
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