CCK-8
Original Article Silence of Beclin1 in oral squamous cell carcinoma cells promotes proliferation, inhibits apoptosis, and enhances chemosensitivity
... To investigate the effects of Beclin1 on cell proliferation, the CCK-8 assay and colony for- mation assay were performed and a growth curve generated. The proliferation of KB and CAL-27 cells infected with ...
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Artesunate prevents knee intraarticular adhesion via PRKR-like ER kinase (PERK) signal pathway
... The Cell Count Kit-8 (CCK-8, Dojindo, Tokyo, Japan) was employed for testing fibroblast viability. The fifth to seventh generations of fibroblasts were taken, and the cells were planted in two ...
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<p>Sphingosine kinase 2 inhibitor ABC294640 displays anti-epithelial ovarian cancer activities in vitro and in vivo</p>
... In order to explore the effect of ABC294640 on the behavior of ovarian cancer cells in vitro, we used cell counting kit-8 (CCK-8) assays, colony formation assays, fl ow cytometry, quantit[r] ...
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<p>Knockdown of lncRNA TDRG1 Inhibits Tumorigenesis in Endometrial Carcinoma Through the PI3K/AKT/mTOR Pathway</p>
... tissues and cell lines than that in normal tissues and hEEC cells, indicating the possible involvement of this lncRNA in EC progression. Next, TDRG1 was knocked down by the transfec- tion of si-TDRG1 into EC cells, ...
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MicroRNA-384 inhibits proliferation migration and invasion of glioma by targeting at CDC42
... The miR-384 mimics, miR-384 inhibitor, miR negative con- trol (miR-NC) and inhibitor-NC, as the relative controls, were designed and provided by GenePharma (Shanghai, People’s Republic of China). The following sequences ...
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Small cell lung cancer growth is inhibited by miR 342 through its effect of the target gene IA 2
... hormone; CCK-8: cell counting kit-8; DAPI: 4′,6-diamidino-2-phenylindole, dihydrochloride; DCV: dene-core vesicle; ELISA: enzyme-linked immunosorbent assay; FSH: follicular stimulating hormone; ...
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Effects of a novel cholecystokinin (CCK) receptor antagonist, MK 329, on gallbladder contraction and gastric emptying in humans Implications for the physiology of CCK
... developed CCK receptor antagonist, ...of CCK-8 (30 ...of CCK infusion. MK-329 caused a dose- dependent inhibition of CCK-stimulated gallbladder contraction with 10 mg producing complete ...
7
Original Article Expression of CDC6 in ovarian cancer and its effect on proliferation of ovarian cancer cells
... of CCK-8 showed that after three days of siRNA1 and siRNA2 transfection, OD values of that were significant- ly lower compared with the NC and blank con- trol (P < ...
7
<p>DR7 encoded by human herpesvirus 6 promotes glioma development and progression</p>
... DR7. CCK-8 and soft agar assays showed enhanced proliferation and colony formation in the cells expressing DR7 which might be in relation to acceleration of the G1/S phase transition by ...
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miR-125a-5p suppresses colorectal cancer progression by targeting VEGFA
... cells. CCK-8 assay was used to evaluate the proliferative ability of CRC cells, and the results showed that upregulation of miR-125a-5p significantly inhibited the proliferation of HCT116 and HT29 cells ...
15
Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite
... Methods: HAP crystals were grown under the guide of silk fibroin (SF) PET surface by biomi- metic route. Several techniques including scanning electron microscopy, attenuated total reflec- tance Fourier transform ...
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Anti adipogenic effects of the traditional herbal formula Dohongsamul tang in 3T3 L1 adipocytes
... The mouse fibroblast cell line, 3T3-L1 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and Dulbeco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), newborn calf serum ...
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Legumain Promotes Gastric Cancer Progression Through Tumor-associated Macrophages In vitro and In vivo
... A CCK-8 assay and transwell migration assay indicated that upregulation of LGMN expression in TAMs stimulated cell proliferation, migration and invasion in vitro, while downregulation of LGMN expression ...
9
Anti-EGF Receptor Aptamer-Guided Co-Delivery of Anti-Cancer siRNAs and Quantum Dots for Theranostics of Triple-Negative Breast Cancer
... Cell growth inhibition by transfection with anti-cancer therapeutic siRNAs treated with QLs, aptamo-QLs, and immuno-QLs was measured using the Cell Counting Kit-8 (CCK-8, Dojindo Labo[r] ...
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LncRNA AB209371 up-regulated Survivin gene by down-regulating miR-203 in ovarian carcinoma
... CCK-8 kit (Sigma-Aldrich, USA) was used to analyze the effects of different transfections on the proliferation of UWB1.289 cells. Briefly, UWB1.289 cells were col- lected at 24 h post-transfection, and the ...
6
Vasoactive intestinal polypeptide mediates cholecystokinin induced relaxation of the sphincter of Oddi
... M CCK-8 and 4 X 10(-11)-5 X 10(-7) M VIP generated dose-related sphincter of Oddi relaxation, which was unaffected by atropine, propranolol, and ...of CCK-8 was antagonized by dibutyryl cGMP ...
6
Significant Changes in the Activity of L-Glutamic Acid Decarboxylase of Mouse Hypothalamus After Peripheral Injection of Cholecystokinin-8 and Caerulein
... France). CCK-8 (sulfated form) and CLN were obtained from Peninsula Laboratories Europe ...(England). CCK-8 and CLN were dissolved in physiological saline for ...
5
<p><em>EPS8</em> is a Potential Oncogene in Glioblastoma</p>
... Abbreviations: EPS8, Epidermal growth factor receptor kinase substrate 8; GBM, glioblastoma; CCK-8, Cell Counting Kit-8; NC, untreated U87MG cells.. Yang et al Dove press.[r] ...
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Metformin inhibits 17β-estradiol-induced epithelial-to- mesenchymal transition via βKlotho-related ERK1/2 signaling and AMPKα signaling in endometrial adenocarcinoma cells
... Metformin inhibits the 17β-estradiol-induced proliferation, migration, and invasion of endometrial adenocarcinoma cells The Cell Counting Kit-8 CCK-8 assays were performed to determine t[r] ...
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Overexpression of TGFβ1 in murine mesenchymal stem cells improves lung inflammation by impacting the Th17/Treg balance in LPS-induced ARDS mice
... Blasticidin; CCK-8: Cell counting kit-8; CDS: Coding sequence; DMEM/ F12: Dulbecco ’ s modified Eagle ’ s media/nutrient mixture F-12; EDTA: Trypsin- ethylenediaminetetraacetic acid; eGFP: Enhanced ...
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