cDNA Cloning
cDNA Cloning of Paramyosin from Several Kinds of Squid Mantle Muscle
... instructions. cDNA was synthesized using total RNA and oligo dT primers with a reverse transcriptase (Takara Bio, Shiga, Japan) accord- ing to the manufacturer’s ...instructions. cDNA synthesis parameters ...
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cDNA cloning and mRNA expression of cat and dog <em>Cdkal1</em>
... A cDNA library was prepared from total liver RNA using the SMARTer RACE cDNA Amplifi- cation Kit (Clontech, Mountain View, ...CDKAL1 cDNA sequence (GenBank accession number NM_017774) and the cat ...
5
cDNA Cloning and Expression Analysis of the Chalcone Synthases (CHS) in Osmanthus fragrans
... of cDNA end (RACE) approach was used to isolate the 3’ and 5’ ends of OfCHS cDNA by use of 3’ SMARTer™ RACE cDNA Amplification Kit (Clontech) and 5’ RACE System for Rapid Amplification of cDNA ...
9
DMDL, an autosomal homologue of X-linked dystrophin; gene expression and cDNA cloning
... Bpl22 and Bpl33 were identical to each other in size (1.7Kb) and sequence. Both showed some sequence which was not contiguous with that of other DMDL (UTRN) cDNA clones. The cDNA was partially sequenced, ...
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cDNA cloning of human plasminogen activator inhibitor from endothelial cells
... Full-length cDNA for plasminogen activator inhibitor (PAI-1) was isolated from a human umbilical vein endothelial cell (HUVEC) lambda gt11 cDNA library. Three overlapping clones were identified by ...
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Purification and cDNA cloning of the ovigerous hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir
... The primary structure of the OHSS gene was examined. A series of PCRs were conducted using genomic DNA as a template with a set of two gene-specific primers (F1 and R1 in Fig.·3). The primers were designed to correspond ...
12
Sheep lung cytochrome P4501A1 (CYP1A1): cDNA cloning and transcriptional regulation by oxygen tension
... oxygen-induced lung injury. The P4501A1 (CYP1A1) isozyme is present in many lung cells, including endothelial cells, and may therefore be involved in the pathogenesis of hyperoxic injury to microvascular endothelium. ...
8
cDNA Cloning, Prokaryotic Expression of Two Splicing Products of mLRG, a Mouse Gene of Lipopolysaccharide Response
... NIH3T3 cDNA first-strand construct as template and two franking primers(PF, PR), The PCR reaction was performed using standard protocols with 35 cycles of 30 s at 94 ˚C, 45 s at ...multiple cloning site of ...
9
Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization
... out each using 0.1 μg of the 5′-RACE-Ready cDNA or 3′-RACE-Ready cDNA as a template. The primer pairs in each reaction were UPM and ClPDI-6R primers, and UPM and ClPDI-7 F primers. A 1.3 kb fragment (5′- ...
7
cDNA Cloning and Overexpression of Acidic Ribosomal Phosphoprotein P1 Gene (RPLP1) from the Giant Panda
... the cDNA sequence of RPLP1 gene from the total RNAs extracted from the skeleton muscle of the Giant Panda, and then analyzed the sequence characteristics of the protein encoded by the cDNA and compared it ...
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Thyroid hormone regulated brain mitochondrial genes revealed by differential cDNA cloning
... years whether thyroid hormone could affect brain development before birth 23 and the generalized idea was that T3 was not required during fetal life, our results show that thyroid hormon[r] ...
8
Cell-based expression cloning for identification of polypeptides that hypersensitize mammalian cells to mitotic arrest
... cell-based cDNA cloning method to isolate proteins and protein fragments whose expression inhibits colony formation in the presence of microtubule ...
8
The insulin receptor and the molecular mechanism of insulin action
... This was further validated by the cDNA cloning, which indicated that the intracellular domain of the a-subunit of the insulin receptor is highly homologous with several other tyrosine pr[r] ...
7
Characterisation of the genomic region upstream of PSG 11 : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University
... Zimmermann, W., Weiss, M.,Thompson, J.A.: cDNA cloning demonstrates the expression of pregnancy-specific glycoprotein genes, a subgroup of the carcinoembryonic antigen gene family, in fe[r] ...
138
Molecular Cloning and Tissue Distribution of Troponin C from the Japanese Pearl Oyster, Pinctada fucata
... by cDNA cloning and compared with those from mollusks using ClustalW: akazara scallop Chlamys nipponensis akazara (BAA12908), asari clam Ruditapes philippinarum (AFB83400), and squid Todarodes pacificus ...
12
Molecular basis of hypoxanthine guanine phosphoribosyltransferase deficiency in ten subjects determined by direct sequencing of amplified transcripts
... Mutant HPRT gene sequences from patients deficient in enzyme activity have previously been characterized by cDNA cloning or amino acid sequencing techniques.. The presence of HPRT-specif[r] ...
6
Towards understanding EC coupling protein expression with age
... "Molecular cloning of cDNA encoding the Ca2+ release channel ryanodine receptor of rabbit cardiac muscle sarcoplasmic reticulum." The Journal of biological chemistry 26523: 13472-13483..[r] ...
261
Genetic and pharmacological evidence for more than one human steroid 5 alpha reductase
... isolated cDNA clones that encode a human steroid 5 alpha-reductase ...this cDNA is normal in subjects with the genetic disease steroid 5 alpha- reductase ...the cDNA-encoded enzyme exhibits a neutral ...
9
Two conserved oligosaccharyltransferase catalytic subunits required for N-glycosylation exist in Spartina alterniflora
... the cDNA of SaSTT3A and SaSTT3B driven by CaMV 35S promoter completely or partially rescued Arabidopsis stt3a-2 mutant salt-sensitive phenotype, indicating STT3A functions conservatively between glycophyte and ...
12
In vitro exploration of interactions between junctin and the Ryanodine receptor from skeletal and cardiac muscle
... Molecular cloning of cDNA encoding human and rabbit forms of the Ca2+ release channel ryanodine receptor of skeletal musc le sarcop lasmic reticulum.. Molecular cloning of cDNA encoding [r] ...
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