Cell culture isolated cells
Mutational dynamics of the SARS coronavirus in cell culture and human populations isolated in 2003
... Competing interests None declared. Authors' contributions VBV and ETL performed most of the data analysis and pre- pared the draft manuscript. STSY and LAE provided SARS- CoV samples from patients and Vero cells. ...
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Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro
... satellite cells for nuclear transfer, sex detection of transgenic cell lines isolated from single cell cloning is necessary because the gender of transgenic embryo can be determined by sex ...
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ATCC PRIMARY CELL Culture Guide. tips and techniques for culturing primary cells
... PRIMARY CELL SOLUTIONS ® Guide to Culturing Human Primary Cells Primary cells and cell types Primary cell cultures more closely mimic the physiological state of cells in vivo and ...
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A comparison of drug transport in pulmonary absorption models: isolated perfused rat lungs, respiratory epithelial cell lines and primary cell culture
... the cell-free culture support was subtracted from the gross resistance to yield the TER of the epithelial cell ...layers. Cell layers with TER > 200 Ω cm 2 were used in trans- port experiments ...
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Cells Isolated from Human Periapical Cysts Express Mesenchymal Stem Cell-like Properties
... methods Cell preparation and culture Human periapical cystic tissue was obtained from 12 healthy male volunteers, 25 ± 3 years of age, who provided informed consent at the Calabrodental dental clinic ...
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Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system
... stem cells generation from neonatal mouse testis Materials and Methods: Isolated testicular cells were cultured in ...the isolated cells and obtained ES-like cell were immune- ...
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Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis
... synovial cells is the in vitro expansion of synovium-derived, plastic-adherent cell populations, mostly fibroblast-like ...these cells can be counted as circumstantial evidence for mechan- isms ...
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Cell cell signaling between adult rat ventricular myocytes and cardiac microvascular endothelial cells in heterotypic primary culture
... endothelial cells and muscle cells within ventricular myocardium, known to be important during cardiac development, remains physiologically relevant in the adult ...their cells of origin within ...
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Alkaline phosphatase positive cells isolated from human hearts have mesenchymal stem cell characteristics
... ALPL+ cells also expressed troponin T and ABCG2 in 2 of the 3 samples ...heart-derived cells. The success rate for cell isolation by explant culture was higher for auricle-de- rived ...
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Co-culture of placental explants with isolated CD4 and CD8 T cells: a functional model to define the consequences of placental inflammation
... Competing interests: The authors have declared that no competing interests exist. Received August 28, 2015; Revision received November 14, 2015; Accepted December 9, 2015; Published December 22, 2015 Abstract Appropriate ...
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Isolation of common carp ovarian follicular cells and evaluation of their endocrine activity in primary cell culture
... major cell layers; an outer theca layer and an inner granulose (Unal et ...follicular cells of fish oocytes show steroidogenic enzymes activity (Galas et ...Tissue culture of isolated ...
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TEMPERATURE-SENSITIVE VARIANTS OF NICOTIANA TABACUM ISOLATED FROM SOMATIC CELL CULTURE
... Two of the variants adapted from growth on solid medium to growth in a liquid suspension cul- ture; these were further characterized for chromosome number, growth rate[r] ...
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Mechanisms of the proliferation and differentiation of plant cells in cell culture systems
... Jnt J, Dc\' BioI 'X 287 21)9 (199 t) 287 Rrvirw Mechanisms of the proliferation and differentiation of plant cells in cell culture systems HIROO FUKUDA, MASAKIITO', MUNETAKA SUGIYAMA and ATSUSHI KOMAM[.] ...
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Silicon substrate as a novel cell culture device for myoblast cells
... PV cell have been used as a clean renewable source of ...the cell culturing and cell growth using of PV ...for culture of cells on porous 2-D surface. In future, cells loaded on ...
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Cell culture-based analysis of postsynaptic membrane assembly in muscle cells
... 100mm culture dishes coated with laminin for biochemical ...tissue culture dishes were transfected one day post-plating with GFP- RhoN19 plasmid at a final concentration of 5µg of DNA/ml using Lipofectamine ...
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CONTACT-INHIBITED REVERTANT CELL LINES ISOLATED FROM SV40-TRANSFORMED CELLS
... In the present study, there is a correlation between large pseudopodia on transformed cells seen in surface replicas and a diminution of alpha filament sheets in [r] ...
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Cancer stem cells and cisplatin-resistant cells isolated from non-small-lung cancer cell lines constitute related cell populations
... both cell types. H460 cells expressing the Cherry-fluorescent protein were mixed with CSCs expressing GFP to distinguish the cells of the tumor coming from each ...four cell mixtures tested, ...
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GrowDex STEM CELL CULTURE GUIDE. tips and techniques for culturing stem cells
... to culture ESCs It is possible to culture human embryonic stem cells (ESCs) in a 3D feeder free ...the culture and expansion of small colonies of WA07 ESCs embedded in ...
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Cell Culture Experiments
... Medium (MEM) or RPMI 1640 includes vitamins and amino acids and is usually supplemented with various necessary components such as horse serum and/or fetal calf serum and glucose. The inclusion of animal sera to the ...
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Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device
... stromal cells (MSCs) are multipotent and have great potential in cell ...these cells can rescue fulminant hepatic ...static culture method neither maintains growth factors at an optimal level ...
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