cell factory
A novel cell factory for efficient production of ethanol from dairy waste
... a cell factory, due to properties such as its high glycolytic flux, ability to metabolize a broad range of carbohydrates, well-characterized metabolic network, and ease of genetic manipulation ...a ...
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The bag or the spindle: the cell factory at the time of systems' biology
... The second level of expression profiling is, of course, that of the direct access to the gene products, the proteins. Two-dimensional gel electrophoresis has been developed for thirty years, with considerable success, ...
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Bacillus subtilis: from soil bacterium to super-secreting cell factory
... underscored by recent studies on the Tat interactome [30,38], the larger interaction networks in which the se- cretion machinery is placed must be better understood for rational secretion machinery engineering according ...
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Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes
... quality protein complexes and membrane proteins, and on enhancement of the activity of those systems that facil- itate the assembly of these proteins. This will require the characterization and engineering of (1) the ...
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Physiological characteristics of the extreme thermophile Caldicellulosiruptor saccharolyticus: an efficient hydrogen cell factory
... and is relatively tolerant to high partial hydrogen pressures, making it a promising candidate for exploitation in a biohydrogen process. The behaviour of this Gram-positive bacterium bears all hallmarks of being adapted ...
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Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides
... Because of nutritional value and medicinal applica- tions many groups are constructing engineered DGLA producing microbes such as recombinant Aspergillus oryzae, Saccharomyces cerevisiae and Mortierella alpina but all of ...
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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
... functionally describe the A. niger predicted proteome. Of the 14,165 predicted proteins, approximately half (6,505) could be assigned to functional protein classes relating to metabolism, cellular transport and protein ...
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Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience
... stem cell expansion according to a GMP grade ...(the cell factory) in a synergy of skills and experiences that, although coming from two “different worlds”, end up finding a meeting point in the ...
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A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory
... In this study, we have established a manually curated genome-scale metabolic model of L. reuteri JCM 1112, referred to as Lreuteri_530, and validated it with experi- mental data. We identified several knowledge gaps in ...
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A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production
... Background: A stereoisomer of inositol, scyllo‑inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer’s disease. However, this compound is relatively rare, whereas another stereoisomer of ...
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Yeast as a cell factory: current state and perspectives
... modern cell factory, fulfilling the requirements for next generation ...yeast cell factories will be assembled into genetic modules for fast transfer between ...
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Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
... Three types of cellulase genes and specific transcription factors were successfully over-expressed simultaneously in recombinant A. oryzae. To this point, only a few reports have focused on the co-expression of more than ...
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Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory
... host cell for recombinant protein produc- ...higher cell den- sities can generate cellular stresses leading to modifica- tions in membrane composition [27] and that this modified environment influences the ...
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Mesonephric kidney a stem cell factory?
... Hematopoiesis is a well-known model for stem cell differentia- tion. Until recently, the initial hematopoietic activity has been assigned to the yolk sac, then it was supposed to shift via fetal liver and spleen ...
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A simple method to control glycolytic flux for the design of an optimal cell factory
... microbial cell factories are essential to develop economically feasible production processes for various value-added chemicals from renewable biomass at an industrial scale ...of cell factories in the ...
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Post-production protein stability: trouble beyond the cell factory
... Being proteins flexible molecules suitable to be re- designed by genetic methods, upstream protein engi- neering, one of the main approaches to prevent aggrega- tion during production (Table 1, up), is also useful to ...
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Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory
... the different strain backgrounds or product categories, and even unelucidated regulatory mechanisms. So, in our study, the native ERG20 gene was directly mutated and overexpressed using a strong promoter (LCB03). The ...
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Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin
... bacterial cell wall by binding to the N-acyl-D-alanyl- D-alanine terminal of lipid II, thereby terminating the subsequent transpeptidation and transglycosylation reac- tions in the late extracellular stages of ...
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Living together in biofilms: the microbial cell factory and its biotechnological implications
... alter cell-surface prop- erties by increasing the hydrophobicity of the cell, which increases its surface affinity and therefore its initial sur- face adherence [40, 48, ...which cell detachment ...
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Designer rhamnolipids by reduction of congener diversity: production and characterization
... The cell factories described above produce rhamnolip- ids differing in the number of rhamnose moieties, but the mixture of hydroxy-fatty acid chain lengths remains ...microbial cell factory to a ...
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