cell-free supernatant
The effect of NaCl substitution with KCl on proteinase activities of cell-free extract and cell-free supernatant at different pH levels and salt concentrations: Lactobacillus acidophilus and Lactobacillus casei
... of cell-free extract and cell-free supernatant of the probiotics Lac- tobacillus acidophilus and Lactobacillus ...ed cell pellets were used to prepare cell-wall ...
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Effects of cell-free supernatant of Lactobacillus acidophilus LA5 and Lactobacillus casei 431 against planktonic form and biofilm of Staphylococcus aureus
... This study was carried out to investigate the stability, antibacterial properties and biofilm removal potential of cell-free supernatant (CFS) of Lactobacillus acidophilus LA5 and Lactobacillus casei ...
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Efficacy of lyophilized cell-free supernatant of Lactobacillus salivarius (Ls-BU2) on Escherichia coli and shelf life of ground beef
... as cell free supernatant produced from Pediococus acidilactici NCDC 252 with EDTA on total viable count and sensory evaluation of chicken carcasses stored at refrigeration ...
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A Study on the Antibacterial Activity of Lactic Acid Bacteria Isolated from Traditional Iranian Milk Samples
... on cell- free supernatant of a 15h old culture by well diffusion ...method. Cell-free supernatant was obtained as described ...
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ISOLATION AND SCREENING OF STREPTOMYCES SP. FROM CORINGA MANGROVE SOILS FOR ENZYME PRODUCTION AND ANTIMICROBIAL ACTIVITY
... 50ml of colloidal chitin broth was incubated with inoculum at 30˚C in the incubator shaker at 150 rpm for 7 days. The cell free supernatant was collected and the assay was performed. Colloidal chitin ...
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Probiotic Bacteria and their Supernatants Protect Enterocyte Cell Lines from Enteroinvasive Escherichia coli (EIEC) Invasion
... Bacteria cell free supernatant preparation To prepare bacteria cell free supernatant of probiotics, 16 strains from the mentioned above sources, were cultivated in MRS broth for ...
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Biodegradation of Pyrethroid-Cypermethrin using Pseudomonas aeruginosa and Detection of its Plant Growth Promoting Properties
... 20mL medium was centrifuged at 10000rpm for 10 minutes. The cell free supernatant was separated and acidified with 2NHCl to pH 2. Then equal volume of acetone was added. The aqueous layer from the ...
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Anti-biofilm activity of an exopolysaccharide from a sponge-associated strain of Bacillus licheniformis
... The active fraction of SP1 cell free supernatant was initi- ally found to be of polysaccharidic composition. Preli- minary spectroscopic investigations indicated the presence of a compound with a ...
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Biocontrol of the root-knot nematode, Meloidogyne incognita, using an eco-friendly formulation from Bacillus subtilis, lab. and greenhouse studies
... B. subtilis isolate B10 recorded highest percentages of juvenile mortality that reached 25.3 and 18.7%, respect- ively, compared with the other isolates (Fig. 4). Nemati- cidal activity of culture broth and ...
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Extracellular Synthesis of Bactericidal Silver Nanoparticles Using Actinobacterium Dagang 5
... from cell free supernatant of Actinobacteriumdagang 5 FT-IR analysis of silver nanoparticles showed intense absorption bands at ...the free amine group or at the cysteine residue and caps the ...
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EXTRACELLULAR BIOSYNTHESIS, CHARACTERIZATION AND CYTOTOXIC EFFECTS OF ZINC OXIDE NANOPARTICLES SYNTHESIZED FROM THE SUPERNATANT OF PROBIOTIC BACTERIUM, BACILLUS AMYLOLIQUEFACIENS CS4
... using cell free supernatant (CFS) of Bacillus amyloliquefaciens CS4 and evaluation of their anticancer and antioxidant ...HeLa cell line with the effective half maximal inhibitory ...
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“Isolation, Identification and Evaluation of Antimicrobial and Cytotoxic Activities of the Marine Fungus Aspergillus unguis RSPG_204” by Faten K. Abd El-Hady, Amr M. Abdou, Mohamed S. Abdel-Aziz, Egypt.
... cancer cell lines including; HELA (cervical), MCF-7 (breast), HCT116 (colon) and HEPG2 ...All cell lines were obtained from the American Type Culture Collection (ATCC, Minisota, ...All cell lines ...
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“In-vitro Anti-diabetic and Cytotoxic Effect of Coral Derived Fungi (Emericella unguis 8429) on Human Colon, Liver, Breast and Cervical carcinoma cell lines” by Faten K. Abd El-Hady, Mohamed S. Abdel-Aziz, Amr M. Abdou, Kamel H. Shaker, Laila S. Ibrahim, Zeinab A. El-Shahid, Egypt.
... The supernatant and mycelial extracts from the static and shake cultures of the fungus (Emericella unguis 8429) isolated from the soft coral Sinularia ...the supernatant static (A) had the highest ...
6
Antibacterial activity of gold nanorods against <em>Staphylococcus aureus</em> and<em> Propionibacterium acnes</em>: misinterpretations and artifacts
... It is worth to mention here that PEG-Cys-GNR suspen- sion has already undergone two rounds of centrifugations during functionalization process and before antibacterial testing; one after PEGylation and another round ...
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Expression profile analysis identifies IER3 to predict overall survival and promote lymph node metastasis in tongue cancer
... IER3, also known as IEX-1, p22/PRG1, Dif-2, or gly96, belongs to the early response gene family, which is involved in cell differentiation and cell proliferation [30]. IER3 is highly expressed in a variety ...
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The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer
... in cell lysates (Additional file 4: Figure S4b) mostly paralleled the mRNA expression data, whereas the amount of cytokines released in the supernatants, with the exception of IL-1α, was very low or undetectable ...
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Encapsulation of Vitamin C by A Double-Layer Zein/Chitosan Structure with Improved Stability and Controlled Release
... Vitamin C (ascorbic acid, VC), a weak sugar acid, is an essential nutrient in human body which has been proved to be associated with many physiological processes of humans and animals [1,2]. It is one of the most ...
7
Bioleaching of copper from waste printed circuit boards by bacteria-free cultural supernatant of iron–sulfur-oxidizing bacteria
... Figure 2 shows the leaching results of copper from PCBs by bacteria-free cultural supernatant. As shown in Fig. 2a, the addition of PCBs would cause the rise of pH. It indicated that the PCBs was alkaline, ...
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Dimers and complexes with p53 are the prevalent oligomeric forms of a transforming nonkaryophilic T antigen of simian virus 40.
... aureus, the free NKLT was immunoprecipitated from the supernatant with hamster anti-T serum, and both series of precipitates were electrophoresed in polyacrylamide-sodium dodecyl sulfate[r] ...
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Treatment of the bone marrow stromal stem cell supernatant by nasal administration—a new approach to EAE therapy
... BMSC supernatant group at the peak stage of EAE were anesthetized and eutha- nized with pentobarbital and transcardially perfused with saline to eliminate the blood and then with buffered 4% ...
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