CELL LINES AND CULTURE
Scalable culture strategies for the expansion of patient-derived cancer stem cell lines
... Microcarrier-based culture was also suitable for the expansion of CRC cells, and the highest increase in cell con- centration (>3-fold) was observed for PPlus 102-L, Fact 102-L, CGEN 102, and Pro-F 102-L ...
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Variability in APOE genotype status in human-derived cell lines: a cause for concern in cell culture studies?
... Cell lines derived from humans or other animals are commonly used tools which dominate many research fields in the basic and applied life ...conducting cell culture experiments, rather than in ...
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Ideal sphere-forming culture conditions to maintain pluripotency in a hepatocellular carcinoma cell lines
... Huh7 cell lines from patients ...stem cell markers and cell separation ...efficiently culture CSCs from cancer cells are lacking, and more effective culture conditions are ...
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CELLULAR SELECTION AND PARTIAL CHARACTERISATION OF GLADIOLUS CELL LINES RESISTANT TO CULTURE FILTRATE OF FUSARIUM OXYSPORUM
... of culture filtrate of Fusarium oxysporum ...the culture medium for selection of resistant cell ...of culture filtrate for 3 weeks and then characterised using biochemical ...Selected ...
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Najm, Nour Addeen (2012): In vitro culture studies of tick cell lines: "Endosymbionts in tick cell culture and evaluation of media conditions". Dissertation, LMU München: Tierärztliche Fakultät
... tick cell cultures In vitro culture systems, especially continuous cell lines, represent a useful tool for studying many aspects of tick and tick-borne pathogen research (Bell-Sakyi, et ...
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Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii)
... isolation, cell attachment, limitations in nutrient or substrates, and growth compe- tition between different ...two cell types are eventually remained when a cell line forms in most case (Freshney, ...
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Phosphopeptide mapping of proteins ectopically expressed in tissue culture cell lines
... proteins exhibited a more promiscuous dimerization profile forming homodimers and heterodimers with a wide range of class B factors (3-6). With this in mind, we set out a hypothesis that tissue-specific transcriptional ...
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The cell lines used in this study were obtained from the American Type Culture
... on a medium containing 10% fetal bovine serum (Life Technologies, Inc., Rockville, MD, USA) and 100 ng/ml each of penicillin and streptomycin (Life Technologies, Inc.). Boyden chamber assay, wound healing assay and in ...
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High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation
... of culture by carefully separating ICMs from the surrounding TE cells in the blastocysts using a fine pulled-glass nee- ...the culture surface and began to ...in culture were dramatically decreased ...
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Genomic instability of human embryonic stem cell lines using different passaging culture methods
... during culture including the well known duplication ...comparing cell dissociation methods, we found no significant differences between DNA variations number and size, DNA gain or DNA loss frequencies, ...
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Multi-Drug-Resistance in Drug-Naive and Drug- Exposed Ovarian Cancer Cell Lines Responds Differently to Cell Culture Dimensionality
... To conclude, this work demonstrates unique capabilities of CRRC in studying heterogeneity of cell population with respect to MDR activity. Further, our data show that the extent of MDR-transport activation in OC ...
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Action of Lovastatin (Mevinolin) on an in vitro model of angiogenesis and its co-culture with malignant melanoma cell lines
... General outcome Having listed most of the difficulties in using a model that tries to replicate a natural set of circumstances, the results from both models are nevertheless encouraging in that a detectable effect of the ...
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Rice callus suspension culture inhibits growth of cell lines of multiple cancer types and induces apoptosis in lung cancer cell line
... of cell death that can happen simultan- eously especially in the early ...the cell, nucleus, and chromatin condensation and finally formation of apoptotic ...by cell swell- ing with rupture of the ...
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A comparison of drug transport in pulmonary absorption models: isolated perfused rat lungs, respiratory epithelial cell lines and primary cell culture
... The benefit of the simplified IPL described in this study is that it avoids the need for specialized equipment and requires only the skills of an in vivo pharmacologist to isolate and main- tain the lungs ex vivo. The ...
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Vaccinia virus F5 is required for normal plaque morphology in multiple cell lines but not replication in culture or virulence in mice
... While we have not de fined how F5 contributes to plaque formation, its role is non-redundant and its mode of action is distinct from previously de fined mechanisms. Firstly, there was no evidence that F5 plays any role in ...
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Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES 7
... 2003] but the necessity of drug resistant feeders for stable selection of hESCs remains (Vallier et al., 2004). Interestingly, we observed poor EB formation of BG01 and HUES-7 harvested directly from Matrigel, although ...
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Examples Of Cell Lines
... of cell lines that involved discovering nanobodies produced, especially well as ...tissue culture plastic, which not be coated with extracellular matrix components to increase adhesion properties and ...
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Chandipura virus growth kinetics in vertebrate cell lines, insect cell lines & embryonated eggs
... It has already been documented earlier that embryonated eggs yield high virus titre than the cell lines 14 . Our study also demonstrated high virus yield in embryonated eggs than vertebrate and invertebrate ...
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Growth and Maintenance of Insect Cell Lines
... The following protocol will enable you to adapt your cell lines to the appropriate serum- free conditions. The procedure takes about 7 days and involves gradually decreasing the percentage of serum ...
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Culture and Assay Systems Used for 3D Cell Culture
... (PrC) cell lines formed well-differentiated round spheroids, while most PrC lines formed poorly differentiated spheroids or aggressively invading structures in 3D Matrigel matrix cultures 107 ...
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