CFP-10
Prospective Evaluation of a Whole-Blood Test Using Mycobacterium tuberculosis-Specific Antigens ESAT-6 and CFP-10 for Diagnosis of Active Tuberculosis
... A new immunodiagnostic test based on the Mycobacterium tuberculosis-specific antigens CFP-10/ESAT- 6(QFT-RD1) has been launched as an aid in the diagnosis of latent tuberculosis (TB) infection (LTBI). The ...
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Use of Synthetic Peptides Derived from the Antigens ESAT-6 and CFP-10 for Differential Diagnosis of Bovine Tuberculosis in Cattle
... and CFP-10, have been reported to be promising candidates as diagnostic reagents for the detection of ...of CFP-10 that were recognized by ...
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Long-Incubation-Time Gamma Interferon Release Assays in Response to Purified Protein Derivative, ESAT-6, and/or CFP-10 for the Diagnosis of Mycobacterium tuberculosis Infection in Children
... to CFP-10 or PPD or ESAT-6 in the long-incubation-time IGRA was highly suggestive of aTB, with very few LTBI children secreting IFN- ␥ at such ele- vated ...the CFP-10 ...
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Rapid Identification of the Mycobacterium tuberculosis Complex by Combining the ESAT 6/CFP 10 Immunochromatographic Assay and Smear Morphology
... teria. With positive BACTEC cultures that are also AFB pos- itive, colony isolation and identification are carried out using conventional biochemical methods for differentiation of MTC from nontuberculosis mycobacteria ...
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ESAT 6/CFP 10 Fusion Protein and Peptides for Optimal Diagnosis of Mycobacterium tuberculosis Infection by Ex Vivo Enzyme Linked Immunospot Assay in The Gambia
... the CFP-10 peptide antigen is significantly less than that to ESAT-6; in other studies in Zambia (5), India (11), Denmark (2), and The Netherlands (1), the converse was ...the CFP-10 antigens ...
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Construction of Eukaryotic Expression Vectors Encoding CFP-10 and ESAT-6 Genes and Their Potential in Lymphocyte Proliferation
... The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early-secreted target antigen (ESAT-6) are two low molecular weight secretory proteins encoded by the Rv3874 and Rv3875 genes, ...
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Diagnosis of Bovine Tuberculosis by Comparative Intradermal Tuberculin Test, Interferon Gamma Assay and esxB (CFP 10) PCR in Blood and Lymph Node Aspirates
... and CFP-10 proteins re- spectively, present in pathogenic Mycobacterial species, can be used for confir- mation of ...and CFP-10 has also been detected in other mycobacterial species and ...
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Assessment of Cross-Reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-Cell Epitope Level
... and CFP-10 are used in the BOVIGAM gamma interferon (IFN- ␥ ) test, they have also been shown to enhance the specificity of the tuberculin-based test per se in this respect (7, 8, 20, 21, 26, ...and ...
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Molecular and Immunological Characterization ofMycobacterium tuberculosis CFP 10, an Immunodiagnostic Antigen Missing in Mycobacterium bovis BCG
... It has been well established that the generation of substan- tive antibody responses to protein antigens is dependent on the existence of T-cell epitopes within the antigen recognized by helper T lymphocytes. Thus, ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Secreted proteins of M. tuberculosis have been reported as rich source of immunogens (8). In the last decades, these proteins have gained specific attention as virulence factors, vaccine and diagnostic candidates (3, ...
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Selected Pool of Peptides from ESAT 6 and CFP 10 Proteins for Detection of Mycobacterium tuberculosis Infection
... in 10 to 20% of cases Mycobacterium tuberculosis is not successfully cultured) ...Moreover, 10 to 25% of TB patients have a negative TST result, and this per- centage increases up to 50% in patients with ...
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A T cell based assay using specific Mycobacterium tuberculosis antigens, ESAT-6 and CFP 10, for the diagnosis of Tuberculous Meningitis.
... The test was performed in 14 cases with tuberculous meningitis - diagnosis based on standard diagnostic criteria [60] and 10 patients with non-tuberculous meningitis that served as the control samples. All cases ...
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Use of Whole-Blood Samples in In-House Bulk and Single-Cell Antigen-Specific Gamma Interferon Assays for Surveillance of Mycobacterium tuberculosis Infections
... Whole-blood intracellular staining assay. The whole-blood intracellular stain- ing method visualizes cytokines in antigen-activated T cells at the single-cell level and is based on a short stimulation, the inhibition of ...
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MTB-specific lymphocyte responses are impaired in tuberculosis patients with pulmonary cavities
... and CFP-10, the proportions of CD4 + IFN-γ, CD4 + TNF-α, CD8 + TNF-α, and CD8 + IFN-γ cells were all markedly increased in 46 patients with TB when compared with 23 household ...
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Pleural IFN γ release assay combined with biomarkers distinguished effectively tuberculosis from malignant pleural effusion
... antigen; CFP-10: 10 kDa culture filtrate antigen; CRP: C-reactive protein; ELISPOT: Enzyme-linked Immunospot; ESAT-6: Early secretory antigenetic target of 6 kDa; ESR: Erythrocyte sedimentation rate; ...
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IL 17 and IL 22 production in HIV+ individuals with latent and active tuberculosis
... in CFP-10 + ESAT-6 stimulated PBMCs (d, f) before and (e, g) after blocking PD1 in HIV + LTBI+ and HIV + TB+ patients respect- ...in CFP-10 + ESAT-6 stimulated PBMCs (c, e) before and (d, f) ...
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Effect of isoniazid preventive therapy on immune responses to mycobacterium tuberculosis: an open label randomised, controlled, exploratory study
... Net cytokine production after subtraction of spontaneous cytokine production was determined in response to TB antigens ESAT-6, CFP-10 and TB7.7 peptide 4 in QFN supernatants tested for c[r] ...
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Evaluation of Gamma Interferon Release Assays Using Mycobacterium tuberculosis Antigens for Diagnosis of Latent and Active Tuberculosis in Mycobacterium bovis BCG-Vaccinated Populations
... Recently introduced IGRAs for M. tuberculosis infection have successfully been developed (1, 20), and their use is ex- panding due to a higher sensitivity and specificity than those of the TST (10, 12, 17, 21, ...
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Assessment of Mycobacterium tuberculosis OmpATb as a Novel Antigen for the Diagnosis of Bovine Tuberculosis
... and CFP-10 have been shown to be outstanding diagnostic target proteins in the whole-blood IFN- ␥ assay for cattle (1, 2, 10, 14, 18, 29, 40, 41, 45) and for humans (3, 8, 15, ...and ...
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Diagnostic Implications of Antigen-Induced Gamma Interferon Production by Blood Leukocytes from Mycobacterium bovis-Infected Reindeer (Rangifer tarandus)
... protein 10 [CFP-10], MPB-59, MPB-64, and MPB-70) have been evaluated for use in tests that discriminate between ...and CFP-10 are particularly robust inducers of recall IFN- ␥ responses ...
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