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Chemical Mutagenesis

Negative effects of chemical mutagenesis on the adaptive behavior of vesicular stomatitis virus.

Negative effects of chemical mutagenesis on the adaptive behavior of vesicular stomatitis virus.

... that chemical mutagenesis could push quasispecies populations near the error ...various chemical mutagens on different types and strains of viruses, investigations of the physiological effects and ...

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Chemical Mutagenesis of Dengue Virus Type 4 Yields Mutant Viruses Which Are Temperature Sensitive in Vero Cells or Human Liver Cells and Attenuated in Mice

Chemical Mutagenesis of Dengue Virus Type 4 Yields Mutant Viruses Which Are Temperature Sensitive in Vero Cells or Human Liver Cells and Attenuated in Mice

... study, chemical mutagenesis of DEN4 was utilized to generate attenuating mutations which may be useful in further attenuation of the 2A ⌬ 30 candidate ...

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INDUCED PHYSICAL AND CHEMICAL MUTAGENESIS AND ITS EFFECT IN CYTOLOGICAL BEHAVIOR OF SORGHUM (SORGHUM BICOLOR (L ) MOENCH)

INDUCED PHYSICAL AND CHEMICAL MUTAGENESIS AND ITS EFFECT IN CYTOLOGICAL BEHAVIOR OF SORGHUM (SORGHUM BICOLOR (L ) MOENCH)

... and chemical are to two fundamentally different types of change produced within the induced ...this chemical composition of the genes, and that they probably are “endless in their eventual ...

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Physical and chemical mutagenesis of VM1 generation in tubers of glory lily (Gloriosa superba)

Physical and chemical mutagenesis of VM1 generation in tubers of glory lily (Gloriosa superba)

... which is originated from tropical Africa, is grown as one of the important medicinal crop in India and other Asian countries like Sri Lanka, Bangladesh, Myanmar and Malaysia. It is mainly cultivated for its seed and ...

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Chemical mutagenesis

Chemical mutagenesis

... Originally the term radiomimetic was used in the sense that the chemical was able to simUlate all the end effects of exposure to ionizing radiations" However, the term ha,s more recently[r] ...

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Effect of chemical mutagenesis in Capsicum annuum L. CvJwalamukhi

Effect of chemical mutagenesis in Capsicum annuum L. CvJwalamukhi

... EMS produce broad range of effects on plants. EMS induced seed mutagenesis in rice (Nair and Ninan, 1973), male sterility in wheat (Maan and Williams, 1984), herbicide tolerance in soybean (Sebastian et al., ...

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Effect of chemical mutagenesis in Capsicum annuum L. Cv Jwalamukhi

Effect of chemical mutagenesis in Capsicum annuum L. Cv Jwalamukhi

... IJSRR, 8(1) Jan. – March., 2019 Page 1330 Induced mutation has become an effective tool to improve a crop through creation of variability. The chemical mutagens produce either mutagenic changes or inactivating ...

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ArhGEF18 regulates RhoA Rock2 signaling to maintain neuro epithelial apico basal polarity and proliferation

ArhGEF18 regulates RhoA Rock2 signaling to maintain neuro epithelial apico basal polarity and proliferation

... RESULTS Morphology, proliferation and apicobasal polarity are affected in medeka mutant retinae We used chemical mutagenesis in medaka fish to identify novel genes that function in epith[r] ...

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Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer

Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer

... including chemical mutagenesis (35–37), group II intron gene disruption systems (29), allele replacement (33, 34, 53), inducible gene expression (28, 54), and targeted gene repression ...random ...

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Gene Deletion by Fluorescence Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis

Gene Deletion by Fluorescence Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis

... via mutagenesis is considered essential for de- finitively assigning function and fulfilling the molecular Koch’s postulates ...including chemical mutagenesis by exposure to EMS or gene disruption ...

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Journal of Applied Pharmaceutical Science

Journal of Applied Pharmaceutical Science

... The aim of the present study is to optimize the growth conditions for improved production of alkaloids by promising marine actinomycetes isolated from marine sediments collected on Kakinada coast. The bioactive compounds ...

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Genotypic and Phenotypic Detection of Mutant Thrombolytic Enzyme (staphylococcal fibrinolysin) Expressed by Mutant Staphylococcus aureus Vancomycin Sensitive S.aureus (VSSA) and Methicillin Sensitive S.aureus (MSSA) by Hydroxylamine Chemical Mutagen

Genotypic and Phenotypic Detection of Mutant Thrombolytic Enzyme (staphylococcal fibrinolysin) Expressed by Mutant Staphylococcus aureus Vancomycin Sensitive S.aureus (VSSA) and Methicillin Sensitive S.aureus (MSSA) by Hydroxylamine Chemical Mutagen

... The chemical mutagenesis done in order to improvement expression of staphylokinase by using Hydroxylamine (HA) in different doses with different methods by induce genetic mutation that alters staphylokinase ...

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Fast Neutron Mutagenesis in Plants: Advances, Applications and Challenges

Fast Neutron Mutagenesis in Plants: Advances, Applications and Challenges

... other mutagenesis approaches viz. chemical mutagenesis which offer more precise single nucleotide polymorphisms ...FN mutagenesis, varying degrees of genetic variations are ...FN ...

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PROPAGATION AND CHEMICAL MUTGENIC INDUCTION OF EUSTOMA GRANDIFLORUM PLANT USING TISSUE CULTURE TECHNIQUE

PROPAGATION AND CHEMICAL MUTGENIC INDUCTION OF EUSTOMA GRANDIFLORUM PLANT USING TISSUE CULTURE TECHNIQUE

... Mutagenesis is one of the powerful tools for breeding biotechnology. Chemical mutagenesis is an easy tool, which doesn’t require special tools and provide high mutation rate. Effects of different ...

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Insertional mutagenesis in transgenic mice generated by the pronuclear microinjection procedure

Insertional mutagenesis in transgenic mice generated by the pronuclear microinjection procedure

... germline mutagenesis has been an underutilized source of new mutations in the ...of mutagenesis, careful screening of transgenic lines will be necessary to detect useful phenotypes caused by the integration ...

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Theory of Lethal Mutagenesis for Viruses

Theory of Lethal Mutagenesis for Viruses

... Lethal mutagenesis is a form of ex- ...lethal mutagenesis is a genetic (evolutionary) phenomenon because it is driven by mutations, it is not an exclusively genetic one because it also depends on absolute ...

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Bacterial anion exchange: reductionist and integrative approaches to membrane biology

Bacterial anion exchange: reductionist and integrative approaches to membrane biology

... scanning mutagenesis to UhpT, we asked whether the cysteines normally present in UhpT are essential to function (see ...Site-directed mutagenesis was used to replace, one at a time, each of the six UhpT ...

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Promoter analysis by saturation mutagenesis

Promoter analysis by saturation mutagenesis

... Primer extension analysis is routinely used for expression profiling (Fig. 5) (1, 2). This method has advantages over other profiling methods like RT-PCR, and Northern blotting in that it is easy, quantitative, and ...

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CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major

CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major

... CRISPR/Cas9 was adapted to Drosophila in 2014 (21) and is now part of the genetic manipulation toolbox in this classical insect model. CRISPR/Cas9 genome-engineering has also been performed with success in A. gambiae and ...

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A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys

A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys

... In this study, a gene of a novel esterase, Lip, was cloned from the marine bacterium Enterobacter cloa- cae and expressed in Escherichia coli (E. coli). The ester- ase showed high hydrolytic activity at low temperature ...

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