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Chemically Defined Medium

Enhanced Spore Production of Bacillus subtilis Grown in a Chemically  Defined Medium

Enhanced Spore Production of Bacillus subtilis Grown in a Chemically Defined Medium

... component, chemically defined medium, was tested for ...production. Medium composition was optimized with re- spect to vitamin composition, carbon, nitrogen and calcium ...

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Transferrin enhances lentoid differentiation in rat egg cylinders cultivated in a chemically defined medium

Transferrin enhances lentoid differentiation in rat egg cylinders cultivated in a chemically defined medium

... Int I De\ Bini 3~ 275 279 (] 990) 275 Original Arlirl, Transferrin enhances lentoid differentiation in rat egg cylinders cultivated in a chemically defined medium FLORIANA BULlC JAKUS', NIKOLA SKREB'*[.] ...

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Effects of selenium, calcium and calcium ionophore on human oocytes in vitro maturation in a chemically defined medium

Effects of selenium, calcium and calcium ionophore on human oocytes in vitro maturation in a chemically defined medium

... Treatment with ITS plus porcine follicular fluid significantly improved the fertilization parameter of oocytes (17). At the final step, embryo development of in vitro matured oocytes evaluated. There was the significant ...

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Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium

Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium

... Addition of cortisol resulted in a potent dose-dependent stimulation of the adipose differentiation process. Cortisol could be replaced by dexamethasone and partly by aldosterone, but not by sex steroids. The proportion ...

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Helicobacter pylori Growth and Urease Detection in the Chemically Defined Medium Ham's F 12 Nutrient Mixture

Helicobacter pylori Growth and Urease Detection in the Chemically Defined Medium Ham's F 12 Nutrient Mixture

... Use of F-12 or its derivatives may lead to improved understanding of H. pylori metabolism, virulence factors, and transmission, and result in improved recovery and identification of H. pylori from clinical specimens. The ...

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Co culture of contiguous developmental fields in a serumless, chemically defined medium: an in vitro model permissive for coordinate development of the mouse ear

Co culture of contiguous developmental fields in a serumless, chemically defined medium: an in vitro model permissive for coordinate development of the mouse ear

... Int I 0 ,' Uiol ~O;953 964 (1996) 953 Original Article Co culture of contiguous developmental fields in a serum less, chemically defined medium an in vitro model permissive for coordinate development[.] ...

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Chemically defined medium for Legionella pneumophila growth

Chemically defined medium for Legionella pneumophila growth

... Development of a chemically defined liquid medium for growth of Legionella pneumophila. Liquid medium for growth of Legionella pneumophila[r] ...

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WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae

WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae

... Moberg (15) reported that strains with requirements for arginine, hypoxanthine, and uracil were predominant among the 166 isolates tested from Stockholm. These data indicated[r] ...

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Growth of Legionnaires disease bacterium (Legionella pneumophila) in chemically defined medium

Growth of Legionnaires disease bacterium (Legionella pneumophila) in chemically defined medium

... In both complex (unpub- lished data) and defined medium (Fig. 3), the absence of iron resulted in a slower growth rate and decreased cell yield. We are currently inves-[r] ...

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Growth of Porphyromonas gingivalis, Treponema denticola, T  pectinovorum, T  socranskii, and T  vincentii in a chemically defined medium

Growth of Porphyromonas gingivalis, Treponema denticola, T pectinovorum, T socranskii, and T vincentii in a chemically defined medium

... The development of medium OMIZ-Wl was a long, iter- ative process during which undefined, complex medium components were progressively replaced by synthetic chem- icals and established l[r] ...

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Proteomics Profiling of Chimeric-Truncated Tissue  Plasminogen activator Producing- Chinese Hamster Ovary  Cells Cultivated in a Chemically Defined Medium  Supplemented with Protein Hydrolysates

Proteomics Profiling of Chimeric-Truncated Tissue Plasminogen activator Producing- Chinese Hamster Ovary Cells Cultivated in a Chemically Defined Medium Supplemented with Protein Hydrolysates

... Background: Culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. Methods: In this study, designing the optimum mixture of four soy and ...

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Nutritional Requirements and Antibiotic Resistance Patterns of Helicobacter Species in Chemically Defined Media

Nutritional Requirements and Antibiotic Resistance Patterns of Helicobacter Species in Chemically Defined Media

... philosophy should be applied to the growth of H. pylori in chemically defined, protein-free media. To date, little has been published regarding the nutritional requirements of other Helicobacter species. ...

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The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium

The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium

... a chemically defined medium to systemically examine cellular activities and the impact of medium components in high-density ...that medium acidosis is the main factor that alters cell ...

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Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions

Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions

... First we characterized the synthesis of steroid hormones and ultrastructural features of the GCs in the chemically defined medium supplemented with PVA. A time-course analysis for E2, P3, and P4 ...

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Development of a chemically defined liquid medium for growth of Legionella pneumophila

Development of a chemically defined liquid medium for growth of Legionella pneumophila

... The CDLp broth, having 0.01% of each of 19 amino acids, supported growth and cell yields of the tested strains to a degree only 0.2 OD units less than that obtained with the SSLp medium [r] ...

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Optimizing chemically-defined culture medium for the production of bovine  (Bos taurus) embryos in vitro

Optimizing chemically-defined culture medium for the production of bovine (Bos taurus) embryos in vitro

... Optimization Medium culture ...maturation medium for 22 ...Optimization Medium culture media and supplemented with bovine serum albumin and fetal calf ...Optimization Medium + 5% FCS for 4-7 ...

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Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells

Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells

... xeno-free chemically defined media to cryopreserve hAECs with a simple slow freezing ...freezing medium also contains high concentrations of FBS, which increases not only the risks of undesired ...

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Identification of Neisseria by electron capture gas liquid chromatography of metabolites in a chemically defined growth medium

Identification of Neisseria by electron capture gas liquid chromatography of metabolites in a chemically defined growth medium

... The medium used in EC-GLC studies was Neisseria defined fluid medium (NEDF) prepared as described (15), except as follows: (i) stock solutions 1, 3, 5, and 6 were autoclaved, whereas all[r] ...

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Approaches to Improve Chemically Defined Synthetic Peptide Vaccines

Approaches to Improve Chemically Defined Synthetic Peptide Vaccines

... these defined pep- tide vaccines have strong vaccination capacity, the mechanisms underlying these improvements are only understood to a basal ...of chemically altered vaccines on the events unfolding ...

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Automation of 3D Cell Culture Using Chemically Defined Hydrogels

Automation of 3D Cell Culture Using Chemically Defined Hydrogels

... is chemically defined and highly reproducible in composition, and (2) the basic material is cell inert; bioactive molecules such as cyto- kines, growth factors, and cell adhesion sites are absent and can be ...

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