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chemostat culture

Large-scale production of magnetosomes by chemostat culture of Magnetospirillum gryphiswaldense at high cell density

Large-scale production of magnetosomes by chemostat culture of Magnetospirillum gryphiswaldense at high cell density

... large-scale cultivation will require precise electrodes for measurement of DO. The oxygen electrodes presently used in large fermentors are not sufficiently sensitive for culture of magnetotactic bacteria. To ...

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Chemostat culture systems support diverse bacteriophage communities from human feces

Chemostat culture systems support diverse bacteriophage communities from human feces

... the chemostat cultures might also persist over ...to chemostat cul- ...the chemostat cultures (Additional file 3: Figure ...the chemostat culture viromes with those of the ...the ...

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Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability

Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability

... Chemostat culture samples (15 ml) were taken for proteomic analyses at all steady states. Samples were centrifuged at 48C (10,000 3 g, 10 min) and the supernatant removed. The pellet was washed with 50mM ...

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The influence of the growth environment on the stability of a drug resistant plasmid in Escherichia coli K12 grown in chemostat culture

The influence of the growth environment on the stability of a drug resistant plasmid in Escherichia coli K12 grown in chemostat culture

... In a repeat experiment (selection experiment number 2) growth in a drug-free carbon-limited medium was maintained for 1800 h. Figure 5*2.1 shows that the number of tetracycline resistant organisms beg.m to decline 500 h ...

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Evolution of Escherichia coli During Growth in a Constant Environment

Evolution of Escherichia coli During Growth in a Constant Environment

... Growth kinetics of the evolved strains: T h e growth rate in chemostat culture is generally consid- ered to be dependent on two parameters, the maxi- mum specific gro[r] ...

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Evaluation of bactericidal activity of Hannon honey on slowly growing bacteria in the chemostat

Evaluation of bactericidal activity of Hannon honey on slowly growing bacteria in the chemostat

... Abstract: There is renewed interest in the therapeutic use of honey, including use in the treatment of infected wounds and burn patients. In this study, we have assessed the antibacterial activity of Libyan floral Hannon ...

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Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis

Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis

... The budding yeast S. cerevisiae is an important biotechno- logical organism e.g. for production of ethanol [1], recom- binant proteins [2], antibiotics [3] or biomass [4]. Additionally it is relevant as a model system to ...

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A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

... oleovorans during growth in batch and chemostat culture with different carbon sources.. Tailored biosynthesis of olefinic medium- 1225[r] ...

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Stable coexistence of two Caldicellulosiruptor species in a de novo constructed hydrogen-producing co-culture

Stable coexistence of two Caldicellulosiruptor species in a de novo constructed hydrogen-producing co-culture

... level [31,33], they are expected to compete for the same growth-limiting substrate in a chemostat culture. Based on the competitive exclusion principle [47], this compe- tition should result in one strain ...

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Plasmid maintenance in Escherichia coli K 12

Plasmid maintenance in Escherichia coli K 12

... The second strategy used nonselective chemostat culture of a strain of E.coli carrying derivatives of pBR322 encoding stability functions from either plasmid pSClOl, the partitioning reg[r] ...

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Global dynamics of a delayed chemostat model with harvest by impulsive flocculant input

Global dynamics of a delayed chemostat model with harvest by impulsive flocculant input

... It was found that some microbes can produce flocculation under the action of floccu- lating agents. This phenomenon makes it possible to harvest microbes by flocculating agents [–]. Recently, based on a classical simple ...

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Metabolic-Flux Profiling of the Yeasts Saccharomyces cerevisiae and Pichia stipitis

Metabolic-Flux Profiling of the Yeasts Saccharomyces cerevisiae and Pichia stipitis

... glucose-limited chemostat cultures using metabolic-flux ratio analysis by nuclear magnetic reso- ...in chemostat cultures of both yeasts is primarily ...batch culture, we demonstrate that the PP ...

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Optimal proteome allocation strategies for phototrophic growth in a light-limited chemostat

Optimal proteome allocation strategies for phototrophic growth in a light-limited chemostat

... light-limited chemostat therefore allows us to com- putationally investigate and compare different possible proteome allocation strategies, such as maximization of growth rate versus maximization of culture ...

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Analysis of a chemostat model in the presence of viral infection expressed by a general infection rate

Analysis of a chemostat model in the presence of viral infection expressed by a general infection rate

... The current research aims at providing a further analysis of the viral infection on the aquat- ic population and the coexistence of uninfected and infected population in a chemostat. The general nutrient uptake ...

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Dynamic behaviors for a model of predator-prey in the chemostat with microbial impulsive inputs

Dynamic behaviors for a model of predator-prey in the chemostat with microbial impulsive inputs

... a chemostat model with n species competing for a single limiting substrate), pulsed input mi- croorganism is more practical and economic than continuous input microorganism in real ...

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Dynamical analysis of multi nutrient and single microorganism chemostat model in a polluted environment

Dynamical analysis of multi nutrient and single microorganism chemostat model in a polluted environment

... The chemostat is a laboratory apparatus used for the continuous culture of microorgan- ism, and it plays an important role in exploring the growth of microorganism in a deter- ministic environment ...the ...

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Biomass yield dependence on inhibitory substrate concentration : a thesis presented as a requirement for the degree of Master of Technology in Biotechnology at Massey University

Biomass yield dependence on inhibitory substrate concentration : a thesis presented as a requirement for the degree of Master of Technology in Biotechnology at Massey University

... Linear regression of yield on substrate was performed for growth on 2,4-D and PCOC in batch configuration and PCOC in chemostat configuration.. Fitting of the linear functional form was [r] ...

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Coexistence of an unstirred chemostat model with B D functional response by fixed point index theory

Coexistence of an unstirred chemostat model with B D functional response by fixed point index theory

... unstirred chemostat model with B-D functional response is studied by fixed point index theory in our ...of chemostat model by degree theory and the homotopy invariance ...

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FREQUENCY-DEPENDENT SELECTION FOR PLASMID-CONTAINING CELLS OF ESCHERICHIA COLI

FREQUENCY-DEPENDENT SELECTION FOR PLASMID-CONTAINING CELLS OF ESCHERICHIA COLI

... Cells containing the derived plasmid pML31 were grown to numerical equilibrium in a glucose-limited chemostat and plasmid-free cells (RH202) were then added such that their initia[r] ...

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SPECIFIC DELETION OCCURRING IN THE DIRECTED EVOLUTION OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN ESCHERICHIA COLI

SPECIFIC DELETION OCCURRING IN THE DIRECTED EVOLUTION OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN ESCHERICHIA COLI

... T h e gnd gene codes for 6-phospho- gluconate dehydrogenase (GPGD), and in a chemostat in which the growth of the cells was limited by the amount of gluconate, a strai[r] ...

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