Chromatin assembly
Tousled kinase TLK1B mediates chromatin assembly in conjunction with Asf1 regardless of its kinase activity
... in chromatin assembly in ...on chromatin assembly in ...tin assembly, but once started, the function of TLK1 could be less catalytic and more like a ...
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Methods designed for the identification and characterization ofin vitro andin vivo chromatin assembly mutants inSaccharomyces cerevisiae
... investigate chromatin assembly in the model system, Saccharomyces ...vitro chromatin assembly assay has been developed and utilized to screen yeast mutants for defects in chromatin ...
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Temporal Dynamics of Cytomegalovirus Chromatin Assembly in Productively Infected Human Cells
... Immunofluorescence microscopy. MRC-5 fibroblasts on no. 1 coverslips (18 by 18 mm) were mock infected or infected with CMV. At 8 to 72 h postinfection, the cells were rinsed briefly in PBS with 0.05% Tween 20 (PBS-T), ...
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Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma
... Abstract: Several studies have revealed that the abnormal expression of chromatin assembly fac- tor 1, subunit A (P150) (CHAF1A) was involved in the development of some types of malignant tumors. However, ...
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Chaperone mediated chromatin assembly and transcriptional regulation in Xenopus laevis
... of chromatin, is large enough to support the approximately 4,000 cells in stage 9 ...complete chromatin assembly through ZGA or if new histone translation from stored mRNAs is necessary for ...
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Chromatin assembly and transcriptional cross talk in Xenopus laevis oocyte and egg extracts
... of chromatin assembly in egg extract re- vealed apparently complete supercoiling by 1 ...probe chromatin assembly rate and density, we probed assembled plasmid DNA in egg extract at 1 and 5 h ...
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Chromatin Assembly on Herpes Simplex Virus 1 DNA Early during a Lytic Infection Is Asf1a Dependent
... Chromatin immunoprecipitation (ChIP) experiments suggest that nucleosomes are present in smaller amounts on HSV than cellular DNA (18, 20, 21, 24, 39). Furthermore, during lytic infec- tion, it has been suggested ...
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The Ubiquitin-Dependent Targeting Pathway in Saccharomyces cerevisiae Plays a Critical Role in Multiple Chromatin Assembly Regulatory Steps
... one chromatin assembly ...Rsp5p-dependent chromatin assembly pathway or repress transcription through chromatin modifica- ultimately effects histone metabolism: Our results dem- tions ...
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Role of Saccharomyces cerevisiae Chromatin Assembly Factor-I in Repair of Ultraviolet Radiation Damage in Vivo
... In vitro, the protein complex Chromatin Assembly Factor-I (CAF-I) from human or yeast cells deposits histones onto DNA templates after replication. In Saccharomyces cerevisiae, the CAC1, CAC2, and CAC3 ...
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Contribution of CAF-I to Anaphase-Promoting-Complex-Mediated Mitotic Chromatin Assembly in Saccharomyces cerevisiae
... vitro chromatin as- sembly assays on extracts prepared from the mutants shown in ...Mild chromatin assembly defects were observed in extracts prepared from single CAF-I mutants ...
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ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization
... RC chromatin assembly in Xenopus egg extracts, where other H3–H4 histone chaperones, such as N1/N2, could possibly pro- vide replicative histones to the CAF-1 complex ...parental chromatin ahead of ...
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Chromatin assembly factor CAF 1 is required for cellular differentiation during plant development
... Chromatin assembly factor CAF-1 facilitates the formation of nucleosomes on newly replicated DNA in vitro. However, the role of CAF-1 in development is poorly understood because mutants are not available in ...
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Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe
... The early description of the CCAN as a stable platform upon which dynamic components of the kinetochore associate during cell cycle progression has been revised in recent years as it has become apparent that some ...
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Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway
... In the current study, we used a reverse-genetics approach, and found that both regulatory subunits are also essential for intra-erythrocytic asexual proliferation. Reasoning that identifying interactors of the regulatory ...
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Introduction of superhelical turns into DNA by adenoviral core proteins and chromatin assembly factors.
... Viral core protein VII or its precursor pVII was incubated with DNA in the presence of an extract of HeLa cell chromatin, which mediates nucleosome assembly from histones and DNA.. We ha[r] ...
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Tax Abolishes Histone H1 Repression of p300 Acetyltransferase Activity at the Human T-Cell Leukemia Virus Type 1 Promoter
... after chromatin assembly, transcription also decreased twofold in the presence of H1-chromatin ...during chromatin assem- bly, CREB-alone activation was repressed twofold by chroma- tin ...
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Novel Interaction between Apc5p and Rsp5p in an Intracellular Signaling Pathway in Saccharomyces cerevisiae
... mitotic chromatin assembly in Saccharomyces ...for chromatin assembly defects, which resulted in the identification of Cdc34p and ...for chromatin assembly defects. Defective ...
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Epigenetic inheritance of an inducibly nucleosome-depleted promoter and its associated transcriptional state in the apparent absence of transcriptional activators
... the chromatin in an Spt6-independent manner upon the heat shock (see ...promoter chromatin assembly and the full transcriptional repression that is mediated by Spt6 were still apparent after adding ...
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Nieberler, Markus (2012): Function of the CHD4/Mi-2ß chromatin remodelling ATPase during neural development of Xenopus laevis. Dissertation, LMU München: Medizinische Fakultät
... marker genes for proliferating neural precursors and differentiated neurons. In addition, I investigated the influence of the CHD4/Sip1 regulator module on neural development by CHD4 misexpression in combination with ...
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ChIP'ing the mammalian genome: technical advances and insights into functional elements
... of chromatin states and nuclear ...native chromatin environ ...of chromatin immunoprecipitation (ChIP) to study chromatin biology in ...and chromatin structures, with high precision ...
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