Cloning and recombinant protein expression
Gene cloning and recombinant protein expression of lipase from marine bacterium
... June 2014 Chairman : Adam Leow Thean Chor, PhD Faculty : Biotechnology and Biomolecular Sciences Nowadays salt tolerant enzymes have gained attention since they have advantages in food processing industries and ...
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Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
... Smsp-4 expression varied seasonally and tended to be higher in summer and lower in winter, suggesting that Smsp-4 plays more important roles in summer than in ...MDa protein complex, comprising six sets of ...
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Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... of recombinant antigens representative of acute or chronic infection might further enhance their application for discrim- inating acute from chronic infection ...yeast expression systems have several ...
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Cloning and Expression of Recombinant Proteins
... Restriction-enzyme based cloning PCR DNA Sequencing Yeast genetics Eukaryotic protein expression systems Transfection of cell culture cells. Insect cell expression[r] ...
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Helicobacter pylori recombinant UreG protein: cloning, expression, and assessment of its seroreactivity
... Akbar Khalilpour 1 , Sabariah Osman 1 , Muhammad Hafiznur Yunus 1 , Amutha Santhanam 1 , Nagarajan Vellasamy 2 and Rahmah Noordin 1* Abstract Background: Helicobacter pylori is a human pathogen and during the process of ...
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Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
... Thus, despite the logic of focusing on membrane proteins as potential antigens for serodiagnosis, other antigens may be superior in terms of antigenicity, species specificity, and possi- bly sensitivity. Comparison of ...
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Cloning, Expression and Characterization of Recombinant Exotoxin A-Flagellin Fusion Protein as a New Vaccine Candidate against Pseudomonas aeruginosa Infections
... research, recombinant exoA-flagellin (fliC) fusion protein as a cocktail antigen was expressed and purified and its antigenic characteristics were ...
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Cloning and Expression of a 48 KilodaltonBabesia caballi Merozoite Rhoptry Protein and Potential Use of the Recombinant Antigen in an Enzyme Linked Immunosorbent Assay
... cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of ...48-kDa protein of ...The recombinant ...
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Cloning, expression and characterization of gE protein of Duck plague virus
... gE protein of duck plague virus is the important membrane glycoprotein, its protein characterization has not been ...the recombinant plasmid ...pET32a/DPV-gE protein was highly expressed after ...
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Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae
... Bacterial cells were collected by centrifugation and were further subjected to sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) to analyze the expression of the recombinant proteins. ...
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Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells
... attractive expression system for the manufacture of therapeutic ...folded recombinant proteins with post-translational ...for protein production in the pharmaceutical industry, we selected this cell ...
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Cloning and characterization of two recombinant Neospora protein fragments and their use in serodiagnosis of bovine neosporosis.
... Variations in antibody responses may be explained by exposure to different stage-specific proteins. While the complete Neos- pora life cycle is unknown, rising Neospora antibody titers dur- ing pregnancy in dams that ...
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Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli
... this protein facilitates the binding of antibody molecules to VP1, and hence VP1 can provoke the immune system of the body ...with recombinant VP1 were protected from the virus infection when ...the ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... The recombinant AcNPV-VP4 DNA was transfected by lipofection into the insect cell line, Spodoptera frugiperda (Sf9) ...cells. Expression of VP4 in the Sf9 cells was confirmed by the immunofluorescence test ...
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Cloning, Phylogenetic Analysis and Expression of Recombinant LipL41, Loa22 and LipL21 Proteins from Leptospira interrogans
... develop recombinant outer membrane protein based diagnostic ...For protein expression truncated lipL41 (1028 bp), loa22 (548bp) and lipL21 (472bp) genes were amplified, cloned and expressed in ...
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Cloning of Aspergillus Niger BglA and expression of recombinant β-glucosidase in methylotrophic yeast Pichia Pastoris
... allow protein engineering work to be performed to improve the properties of this enzyme, it is important to clone and sequence gene encoding this enzyme from different isolates and produce recombinant ...
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Cloning and expression of recombinant human granulocyte-macrophage colony stimulating factor (rhGMCSF) in Escherichia coli
... into expression vector under the control of T7 promoter and was subcloned into ...expressed protein in the form of inclusion body extracted using DDT regenerative ...this protein at low costs is ...
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Cloning and Expression of Recombinant Plasmid Containing P36/LACK Gene of Leishmania infantum Iranian Strain.
... Accepted 12 Jan 2015 Abstract Background: There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular ...
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Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme
... produces expression products that do not form inclusion bodies, and is capable of good protein ...The recombinant enzyme was secreted extracellularly, and we obtained high levels of SPase ...
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Rapid Construction of Recombinant Plasmids by QuickStep-Cloning
... 4 a primer design also facilitates exponential amplification of whole plasmid (4), which results in the production of nicked-circular plasmids, with DNA fragment of interest integrated at the desired position. After a ...
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