Clonogenic Assay
Clonogenic Assay of Type A Influenza Viruses Reveals Noninfectious Cell-Killing (Apoptosis-Inducing) Particles
... of clonogenic assay-generated sur- vival curves of Vero cells exposed to another negative-strand RNA virus, VSV (32), that also kills cell by apoptosis (15, ...
8
A Novel Poly Herbal Formulation Induces Apoptotic Cell Death in Prostate and Colorectal Carcinoma Cells
... Our previous study revealed that Solanum indicum, Rhus succedanea, Rheum emodi and Gardenia gummifera extracts exhibited an anti-proliferation of different five human cancer cells 12 . The present study was carried out ...
17
Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation
... The level of cellular radiosensitivity is almost exclusively assessed by clonogenic assay. Different viability tests, for instance SRB, microtetrasolium (MTT) or BrdU are basi- cally employed for the ...
9
<p>SNHG7 Contributes to the Progression of Non-Small-Cell Lung Cancer via the SNHG7/miR-181a-5p/E2F7 Axis</p>
... proliferation, clonogenic, migration, invasion and ...CCK-8 assay was performed to validate cell proliferation. (E) Clonogenic assay was conducted to detect cell cloning ...Transwell ...
12
Estrogen and soy isoflavonoids decrease sensitivity of medulloblastoma and central nervous system primitive neuroectodermal tumor cells to chemotherapeutic cytotoxicity
... surviving colonies compared to cisplatin alone control cultures (Fig. 3a-b). The cytotoxic effect of 4 μM cis- platin was not significantly changed by the ERα selective agonist PPT (p > .9999). At a final concertation ...
11
Pre-clinical efficacy and synergistic potential of the MDM2-p53 antagonists, Nutlin-3 and RG7388, as single agents and in combined treatment with cisplatin in ovarian cancer
... The clonogenic assay results for combination of RG7388 with cisplatin ranged from antagonism for OAW42, indicating a protective effect of RG7388 against cisplatin, to additive for A2780 and ...
20
Hyaluronic acid-functionalized bismuth oxide nanoparticles for computed tomography imaging-guided radiotherapy of tumor
... (cck8) assay was con- ducted to explore whether any dose enhancement was caused by this combination ...Next, clonogenic assay in SMMC-7721 cells was conducted to evaluate the change in cell viability ...
20
Ivashkevich, Alesia (2008): Investigation into the cellular radiosensitivity of the LEC rat and analysis of candidate genes. Dissertation, LMU München: Fakultät für Biologie
... The radiation sensitivity, as determined by the clonogenic assay, was expressed much less in primary LEC fibroblasts, compared to the data presented by [82]. The conducted study of DSB repair by PFGE (see ...
166
<p>Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells</p>
... live/dead assay, clonogenic assay and observed morphological changes associated with cell death in MCF-7 ...cycle assay in Brv-A treated cells by fl ow ...
16
Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma
... SRB assay. Cell cycle analysis, Hoechst staining, and clonogenic assay were employed to determine the mode of antiproliferative and pro-apoptotic activity in MDA-MB-231 ...
13
miR-205 enhances radiation sensitivity of prostate cancer cells by impairing DNA damage repair through PKCε and ZEB1 inhibition
... the clonogenic assay) was observed in vitro for both cell lines, such a growth delay was likely due to limited local invasive capabilities of miR-205 expressing cells, consistent with our previous finding ...
11
A local effect model-based interpolation framework for experimental nanoparticle radiosensitisation data
... Development and experimental testing of functionalisation high Z NP radiosensitisers for a given cell line is a complex process which can take significant time and resources. Over the last decade, the scientific ...
10
Evaluation of azacitidine and entinostat as sensitization agents to cytotoxic chemotherapy in preclinical models of non-small cell lung cancer
... H358 and A549 cell lines were selected for their ability to form distinct colonies on a reconstituted basement membrane matrix (Matrigel). Using the treatment paradigm previously described, we pretreated cells with ...
15
Hypoxia accelerates vascular repair of endothelial colony-forming cells on ischemic injury via STAT3-BCL3 axis
... In this study, we investigated ex vivo culture conditions for the enhancement of the proliferative, clonogenic po- tential and prevention of ischemic stress-induced apop- tosis in human ECFCs. The optimized ex ...
13
Anticancer activity of 7 epiclusianone, a benzophenone from Garcinia brasiliensis, in glioblastoma
... tion assay was used to determinate cell viability. This assay measures the amount of formazan produced from [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2H-tetrazolium, inner ...
8
Cancer stem cell related markers of radioresistance in head and neck squamous cell carcinoma
... basal at 0 min and also residual γH2AX foci at 24 hours after irradiation than its parental line while the parental and FaDu IR did not differ in the number of residual γH2AX foci (Figure 1D). The lower absolute number ...
16
Nuclear fission safety programme 1992-94: Radiation protection research action, Final report, Volume 2. EUR 16769
... To determine whether the effects produced in the most primitive lympho-hematopoietic selfrenewing stem cells were consistent with data obtained in the clonogenic progenitors, bone marrow[r] ...
1224
Deficiency in clonogenic endometrial mesenchymal stem cells in obese women with reproductive failure – a pilot study
... Obesity is associated with a relative lack of clonogenic stromal cells in cycling endometrium. This may compromise the ability of the uterus to undergo intense tissue remodelling upon embryo implantation, thus ...
8
Clonogenic multiple myeloma cells have shared stemness signature associated with patient survival
... Human MM cell lines RPMI8226 and NCI-H929 were cultured in complete media consisting of RPMI1640, 2mM L glutamine, 50U/ml of Penicillin, 50 µg /ml of Streptomycin and 10% of fetal bovine serum (FBS). Syndican-1 (CD138) ...
11
Mer receptor tyrosine kinase is frequently overexpressed in human non-small cell lung cancer, confirming resistance to erlotinib
... decreased clonogenic growth, improved chemosensitivity, and delayed tumor progression in animal models [5], thus identifying it as a potential therapeutic target in NSCLC ...
14