Clostridium pasteurianum
Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation
... Background: Butanol is currently one of the most discussed biofuels. Its use provides many benefits in compari- son to bio-ethanol, but the price of its fermentative production is still high. Genetic improvements could ...
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Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
... The iron content in the fermentation medium was shown to influence the product formation, especially the 1,3-propanediol and butanol distribution, in Clostridium pasteurianum DSMZ 525 grown on glycerol. ...
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Bio-butanol production from glycerol with Clostridium pasteurianum CH4: the effects of butyrate addition and in situ butanol removal via membrane distillation
... by Clostridium acetobutylicum is a well-known bio-butanol producing process and has been widely used in industry since the early 20th ...the Clostridium pasteurianum strain ...
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Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
... Clostridium pasteurianum can produce n-butanol and 1,3-propanediol (1,3-PDO) with completely different pat- terns from the well-studied ...C. pasteurianum DSMZ 525, n-butanol together with 1,3-PDO is ...
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Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum
... Clostridium pasteurianum. Under certain growth conditions this Clostridium species has been shown to predominantly produce n-butanol, together with ethanol and 1,3- propanediol, when grown on ...C. ...
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A Review of Process-Design Challenges for Industrial Fermentation of Butanol from Crude Glycerol by Non-Biphasic Clostridium pasteurianum
... Cell recycling is a technique used to simultaneously increase the concentration of cells and reaction rate in a fermentation and separate the fermentation broth from the cells for collection. The fermentation broth is ...
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Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme
... and Clostridium cellulolyticum [22] through disruption of the genes encoding the Cac824I and CceI Type II RM systems, ...C. pasteurianum mutant strain obtained using group II intron technology, we rec- ...
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Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growth
... fuels, Clostridium organisms have the major advantage of using a broad spectrum of substrates deriving from biomass (hex- oses, pentoses, oligosaccharides, xylans), and although not extensively studied, Cpa does ...
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Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum
... C. pasteurianum possesses at least one additional restriction-modification system, in addition to CpaAI, that is specifically active on pSY6catP, but not on ...C. pasteurianum which will enable us to ...
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Production of 1,3-PDO and butanol by a mutant strain of Clostridium pasteurianum with increased tolerance towards crude glycerol
... Only few publications have dealt with optimization of glycerol fermentation by C. pasteurianum (Biebl 2001; Dabrock et al. 1992). Recently, a study demonstrating the possibility of utilizing crude glycerol in ...
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Pathogenicity Locus, Core Genome, and Accessory Gene Contributions to Clostridium difficile Virulence
... ABSTRACT Clostridium difficile is a spore-forming anaerobic bacterium that causes colitis in patients with disrupted colonic microbiota. While some individuals are asymptomatic C. difficile carriers, symptomatic ...
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A helicase containing module defines a family of pCD630 like plasmids in Clostridium difficile
... A helicase containing module defines a family of pCD630 like plasmids in Clostridium difficile Accepted Manuscript A helicase containing module defines a family of pCD630 like plasmids in Clostridium[.] ...
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Switchgrass (Panicum virgatum) Fermentation by Clostridium thermocellum and Clostridium beijerinckii Sequential Culture: Effect of Feedstock Particle Size on Gas Production
... Fermentation of cellulosic biomass can be done in a single step with cellulolytic, solventogenic bacteria, such as Clostridium thermocellum. However, the suite of products is limited in consoli- dated ...
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Isolation and Identification of Polyhydroxyalkanoates from two Strains of Clostridium Bifermentans Isolated from the Soil Near the Gas Station in Basrah City
... Amplifying of the 16S rDNA Gene by (PCR) Technique: By using a universal primer, results have obtained the required band of 16S rDNA for the isolate along with electrophoresed ladder in the region of 1500bp, as in the ...
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Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis
... Instead of producing acetate and biomass, the acsA KO strain produced significantly more ethanol, 2,3-butanediol, and lactate than the WT while growing on fructose. Production of these prod- ucts serves as an alternative ...
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Diversity of Clostridium difficile PCR ribotypes in Europe: results from the European, multicentre, prospective, biannual, point-prevalence study of Clostridium difficile infection in hospitalised patients with diarrhoea (EUCLID), 2012 and 2013
... Clostridium difficile infection (CDI) is the major cause of infective diarrhoea in healthcare environments. As part of the European, multicentre, prospective, biannual, point-prevalence study of Clostridium ...
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Accessory Gene Regulator 1 Locus Is Essential for Virulence and Pathogenesis of Clostridium difficile
... Accessory Gene Regulator 1 Locus Is Essential for Virulence and Pathogenesis of Clostridium difficile Accessory Gene Regulator 1 Locus Is Essential for Virulence and Pathogenesis of Clostridium diffic[.] ...
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Microbiome Data Distinguish Patients with Clostridium difficile Infection and Non C difficile Associated Diarrhea from Healthy Controls
... ABSTRACT Antibiotic usage is the most commonly cited risk factor for hospital-acquired Clostridium difficile infections (CDI). The increased risk is due to disruption of the indigenous microbiome and a subsequent ...
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Electron capture gas chromatography study of the acid and alcohol products of Clostridium septicum and Clostridium chauvoei
... The metabolic products produced by several strains of Clostridium septicum obtained from patients and animals, along with strains of Clostridium chauvoei, were studied in chopped meat gl[r] ...
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16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “Clostridium neonatale”
... of Clostridium, “Clostridium ...another Clostridium species involved in neonatal necrotizing enterocolitis, Clostridium ...the Clostridium genus sensu stricto, for which we propose the ...
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