Clostridium thermocellum
Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes
... Clostridium thermocellum DSM 1313 was from Profes- sor Lee ...C. thermocellum cultured in MTC medium [64] and Avicel ® PH-101 at 60 °C and 180 ...
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Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum
... 7]. Clostridium thermocellum is a particularly promising CBP cellulolytic microorganism [3, 6, 8] that is capable of nearly completely digesting cellulose materials such as Avicel on its own ...C. ...
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Addition of β-galactosidase boosts the xyloglucan degradation capability of endoglucanase Cel9D from Clostridium thermocellum
... from Clostridium stercorarium to an in vitro hydrolysis reaction of a model xyloglucan substrate increased the enzymatic efficiency of endoglucanase Cel9D from Clostridium thermocellum to up to ...
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Tracking the cellulolytic activity of Clostridium thermocellum biofilms
... over time (Figure 13) had a characteristic sigmoid shape. Similar profiles are reported in the literature for total off-gas production during fermentation of pure cellulose by mixed ruminal consortia [21] and for carbon ...
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Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation
... Background: Clostridium thermocellum is a promising consolidated bioprocessing candidate organism capable of directly converting lignocellulosic biomass to ...C. thermocellum, we established ...
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Direct glucose production from lignocellulose using Clostridium thermocellum cultures supplemented with a thermostable β-glucosidase
... [1]. Clostridium thermocellum, an anaerobic, thermophilic, spore-forming bacterium, is the most potent cellulose-degrading bacterium known to produce cellulo- somes ...C. thermocellum contain a ...
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Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum
... as Clostridium thermocellum (Ruminoclostridium ther- mocellum) are among the most effectively described bio- catalysts at deconstructing cellulosic biomass [22, 28], and produce ethanol and other reduced ...
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Acetic acid fermentability with Clostridium thermoaceticum and Clostridium thermocellum of standard compounds found in beech wood as produced in hot-compressed water
... Abstract Acetic acid fermentability of various compounds from beech wood as produced in a two-step hot-compressed water treatment was evaluated by fermentation tests using standard compounds with Clostridium ...
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Improved growth rate in Clostridium thermocellum hydrogenase mutant via perturbed sulfur metabolism
... C. thermocellum ∆hydG, whereas wild type remained ...C. thermocellum ΔhydG, 204 and 56 genes were significantly differentially regu- lated relative to wild type in the absence and presence of acetate, ...C. ...
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Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics
... Clostridium thermocellum strains DSM1313 ∆hpt [10], LL1210 [18], M1726 (LL376) [48], M1725 (LL375) [48], and AG1329 [47] were used in this ...C. thermocellum DSM1313 ∆hpt background to create AG1327, ...
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Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase
... [2–4]. Clostridium thermocellum is a good candidate organism for CBP due to its ability to rapidly ferment cellulose and produce ...C. thermocellum to produce etha- nol at higher yield [5] including ...
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Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass
... Clostridium thermocellum switchgrass fermenta- tion samples were measured at 19, 43, 91, and 187 h, as matched for proteomic samples. Frozen cell pellets con- taining both microbe and plant material were ...
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The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
... Clostridium thermocellum strains are routinely isolated from saccharolytic microbial communities [4,35,36], and some lab strains of ...C. thermocellum have turned out to be stable co-cultures of ...
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Switchgrass (Panicum virgatum) Fermentation by Clostridium thermocellum and Clostridium beijerinckii Sequential Culture: Effect of Feedstock Particle Size on Gas Production
... C. thermocellum can be exploited in sequential ...C. thermocellum alone produced 78 kPa cumulative pressure ...C. thermocellum cultures with 15 mm feedstock par- ticles had a mean cumulative pressure ...
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Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress
... anaerobe Clostridium thermocellum is of interest due to its ability to convert biomass cellulose to ethanol ...C. thermocellum produces a large extracellu- lar protein complex called the cellulosome ...
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Expression of adhA from different organisms in Clostridium thermocellum
... Clostridium thermocellum is a cellulolytic anaerobic thermophile that is considered to be a promising can- didate for consolidated bioprocessing of lignocellulosic biomass, into biofuels such as ethanol, ...
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Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
... C. thermocellum DSM1313 accord- ing to the genome editing demand, in which the sequence of HR-short was the same with the 3 ′ region of ...C. thermocellum DSM1313 and Caldicellulosiruptor ...
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The LacI family protein GlyR3 co-regulates the celC operon and manB in Clostridium thermocellum
... Our model of the extended celC regulon is consistent with recent gene expression studies using LacI knock-out strains of C. thermocellum DSM 1313 [23].We compared the expression levels of celC, glyR3, licA, manB, ...
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The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons
... For mass spectroscopy, individual protein bands were cut from an SDS-polyacrylamide gel stained with Coomassie blue, and the in-gel protein was washed, reduced, alky- lated, and digested with trypsin, as described [27]. ...
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The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum
... Hydrolysis was another process shown to be vulner- able to inhibition. Specifically, at the initial 25 and 50 g/L switchgrass loadings, the solubilized, but unfermented glucose equivalents represent 12 and 11%, ...
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