CMV Infected
Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected rhesus macaques.
... of CMV-infected target cells treated with metabolic inhibitors, we identified an E CMV protein (or pro- teins) as the major target recognized by rhesus ...human CMV infection, in which case ...
9
Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis.
... Percentage of nuclei that incorporated [3H]TdR into cellular DNA and exhibited CMVspecific fluorescence after infection" % Nuclei incorporating [3H]TdR in: CMV-infected Mock-infected Pre[r] ...
8
CXCL10 Production from Cytomegalovirus-Stimulated Microglia Is Regulated by both Human and Viral Interleukin-10
... with CMV was found to elicit the production of CXCL10 from primary microglial cells but not from ...from CMV-stimulated microglial cells was partially suppressed by anti-CXCL10 ...cytokines. ...
14
Identification of Cytomegalovirus-Specific Cytotoxic T Lymphocytes In Vitro Is Greatly Enhanced by the Use of Recombinant Virus Lacking the US2 to US11 Region or Modified Vaccinia Virus Ankara Expressing Individual Viral Genes
... total CMV-specific responses. We therefore used recombinant CMV-infected IFN- ␥ -treated fi- broblasts as antigen-presenting cells in ex vivo assays of CMV- specific T-cell responses in immune ...
11
Immunofluorescence for detection of antibodies against human cytomegalovirus induced membrane antigens
... We therefore conclude that membrane fluorescence performed at 0°C on viable CMV-infected cells allows the specific detection of antibodies directed against CMV-MA molecules without inter[r] ...
9
Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody capture enzyme linked immunosorbent assay
... Reactivity against control conjugate was never seen in CMV-infected infants 13 and in sera from AIDS patients but occurred also in sera from a few adults with CMV mononucleosis.. In thes[r] ...
8
Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco
... on CMV- infected tobacco compared to mock-inoculated ...of CMV-infected plants may be a response to decreased nutritional quality of these ...of CMV or CMVD2b infection indicating that ...
7
Improvement of serological diagnosis of neonatal cytomegalovirus infection by simultaneously testing for specific immunoglobulins E and M by antibody capture enzyme linked immunosorbent assay
... This study describes the results of testing 92 serum samples, including 10 umbilical cord serum samples, from 38 cytomegalovirus CMV-infected neonates for CMV-specific immunoglobulin E I[r] ...
5
Cytomegalovirus infection in HIV infected versus non infected infants and HIV disease progression in Cytomegalovirus infected versus non infected infants early treated with cART in the ANRS 12140—Pediacam study in Cameroon
... (CMV infected and CMV uninfected) 1 year after initi- ation of ...HIV infected infants (CMV+ and CMV-) under cART was particularly stud- ied in line with the objective of ...
10
Pathogenesis of congenital cytomegalovirus infection : finding prognostic markers and correlates of protection
... of CMV DNA in neonatal dried blood spot (DBS) at five years of ...congenitally CMV infected children and of 263 non-infected children gave written informed consent for the retrieval of ...
211
Human Cytomegalovirus Modulates Expression of Noncanonical Wnt Receptor ROR2 To Alter Trophoblast Migration
... of CMV infection on the mRNA expression of  -catenin-independent Wnt signaling mediators was studied in human SGHPL-4 trophoblasts using real-time PCR analyses and Western ...blotting. CMV infection ...
8
Antibody mediated bacterial adhesion to cytomegalovirus induced Fc receptors Potential relationship to secondary infections complicating herpesvirus infections
... to CMV-infected cells. To do this, we infected confluent human embryonic lung (HEL) cell monolayers with CMV (strain AD 169) and then used a double radiolabel assay to measure adherence of ...
6
The study of cytomegalovirus infection in vascular smooth muscle cells and in an organ culture of the saphenous vein: Potential role in atherogenesis
... cells infected with CMV under complete transcriptional blockade with actinomycin D are lysed by polyclonal CMV-specific cytotoxic T lymphocytes from all seropositive ...all CMV specific ...
320
Decreased Frequency of Cytomegalovirus (CMV)-Specific CD4+ T Lymphocytes in Simian Immunodeficiency Virus-Infected Rhesus Macaques: Inverse Relationship with CMV Viremia
... in rhesus macaques. Although the ICC assay can detect fre- quencies of antigen-specific cells that are 1 to 2 logs higher than those reported in the earlier literature by LDA, there are few published studies showing a ...
13
Recombinant luciferase expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors
... To produce a recombinant virus expressing the luci- ferase reporter gene under the control of either the pro- moter of an early gene ( POL , UL54) or a late gene (pp28, UL99), the expression cassette of luciferase was ...
7
Optimization of in situ hybridization for detection of viral genomes in cultured cells on 96 microwell plates: a cytomegalovirus model
... Comparison of MAb staining and in situ DNA hybridization for CMV detection in cells cultured on 96-microwell microplates and infected with clinical samples Specimen.. % of positive resul[r] ...
5
Distinguishing Cytomegalovirus (CMV) Infection and Disease with CMV Nucleic Acid Assays
... of CMV nucleic ...Amplicor CMV Monitor and NucliSens CMV pp67 tests were compared to the CMV antigenemia assay for 45 transplant recipients and 1 patient with Wegener’s ...of CMV ...
6
Cytomegalovirus infection of peripheral blood mononuclear cells: effects on interleukin-1 and -2 production and responsiveness.
... Carney and Hirsch 3 showed that human monocytes infected in vivo or in vitro with CMV inhibited the concanavalin A-induced proliferative responses of lymphocytes in subsequent culture.. [r] ...
5
Biliary Atresia, Cytomegalovirus, and Age at Referral
... with which patients with extrahepatic biliary atresia (EHBA) are infected with cytomegalovirus (CMV) and to ascertain the age at referral to a specialty center for sur-.. gical correctio[r] ...
6
Comparison of Three Assays for Cytomegalovirus Detection in AIDS Patients at Risk for Retinitis
... for CMV infection by (i) pp65 antigen- emia expression in leukocytes, (ii) the Digene Hybrid Capture CMV DNA System, and (iii) the Roche Amplicor Qualitative PCR ...of CMV retinitis in our study of ...
6