Co-Immunoprecipitation
A novel method for purifying bluetongue virus with high purity by co immunoprecipitation with agarose protein A
... This study introduced a novel method for purifying BTV-10 by cell debris co-immunoprecipitation with aga- rose Protein A. The logic is pulling out all the compo- nents unwanted, and then the left is what we ...
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Original Article Application of co-immunoprecipitation coupled LC-MS/MS for identification of sperm immunogenic membrane antigens
... of co-immunoprecipitation and liquid chro- matogram mass/mass ...using co-immunoprecipitation ...with co-immunoprecipitation coupled LC-MS/MS analysis could be referred as male ...
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Evidence That the pre-mRNA Splicing Factor Clf1p Plays a Role in DNA Replication in Saccharomyces cerevisiae
... (Dage- Co-immunoprecipitation and chromatin-fractionation stud- MTI, Michigan City, IN) and digitized using an LG-3 video ies: To prepare immune precipitates, ⵑ10 10 cells were ...
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Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae
... inhibitor cocktail Complete (Roche) and 25 U/μl of en- donuclease Benzonase (Merck). Fresh, non-frozen cells were homogenized with glass beads in a bead-beater; the homogenate was spun down in a microfuge. The whole ...
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BHLHE40 confers a pro survival and pro metastatic phenotype to breast cancer cells by modulating HBEGF secretion
... Chromatin immunoprecipitation; CoIP: Co-immunoprecipitation; EGF: Epidermal growth factor; EGFR: Epidermal growth factor receptor; EV: Empty vectors; FR: Fulvestrant-resistant; GF: Glucose depletion; ...
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Fellerer, Christine (2012): Molecular chaperones involved in preprotein targeting to plant organelles. Dissertation, LMU München: Fakultät für Biologie
... using co- immunoprecipitation experiments which highlighted the general role of preprotein complexes involving HSP70, HSP90/HSP70 or ...HSP90 co-chaperones FKBP73, HOP and ...
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The Legionella effector WipB is a translocated Ser/Thr phosphatase that targets the host lysosomal nutrient sensing machinery
... Antibodies and cellular biology reagents. CHIP grade rabbit anti-GFP (Invitrogen) was used for co-immunoprecipitation and mouse anti-GFP (clontech) for Western-blotting. Rabbit anti-vATPase A (Abcam), ...
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A series of N-terminal epitope tagged Hdh knock-in alleles expressing normal and mutant huntingtin: their application to understanding the effect of increasing the length of normal huntingtin’s polyglutamine stretch on CAG140 mouse model pathogenesis
... To determine the extent of potential dominant- negative interactions in vivo, and to begin to explore the effect of expressing a non-pathogenic humanized allele of Hdh encoding htt with a 20Q stretch and human PRR on HD ...
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The git5 Gβ and git11 Gγ Form an Atypical Gβγ Dimer Acting in the Fission Yeast Glucose/cAMP Pathway
... Fission yeast adenylate cyclase, like mammalian adenylate cyclases, is regulated by a heterotrimeric G protein. The gpa2 G␣ and git5 G are both required for glucose-triggered cAMP signaling. The git5 G is a unique ...
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Activation of EGFR-DNA-PKcs pathway by IGFBP2 protects esophageal adenocarcinoma cells from acidic bile salts-induced DNA damage
... Results: Our results demonstrated high levels of IGFBP2 mRNA and protein in EAC cell lines as compared to precancerous Barrett ’ s cell lines, and IGFBP2 is frequently overexpressed in EACs (31/57). Treatment of EAC ...
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HVint: a strategy for identifying novel protein protein interactions in Herpes Simplex Virus Type 1
... 6 Co-Immunoprecipitation (Co-IP), in vitro binding assays, and protein cross-linking, which can be then analyzed by MS. PPIs can also be derived from Nuclear Magnetic Resonance (NMR), X- ray ...
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Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV 1 infection of macrophages in vitro
... During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of ...
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Insights into the Function of the FATC Domain of Saccharomyces Cervisiae TRA1 via Mutation and Suppressor Analysis
... Figure 21: Co-immunoprecipitation assay of myc 9 -tagged tti2 truncation mutations with TAP-tagged Tti1 protein to check for interaction .... 62.[r] ...
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Estrogen receptor α-NOTCH1 axis enhances basal stem-like cells and epithelial-mesenchymal transition phenotypes in prostate cancer
... Additional file 1: Figure S1. A, IF analysis of CD49f and ER α co- expression in prostate tissues, enriched stem cell spheres of LNCaP-abl (PCSCs), LNCaP-abl or PC3 cells. Scale bar, 50 μ m. B, Mean mRNA analysis ...
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Knockdown of menin affects pre-mRNA processing and promoter fidelity at the interferon-gamma inducible IRF1 gene
... We do not observe any change in the enrichment of H3K4me3 at the IRF1 promoter when MEN1 is depleted. Nor do we observe any loss in total IRF1 RNA levels. These dissimilar observations might reflect differ- ences in the ...
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DNA methylation and epigenomics: new technologies and emerging concepts
... chromatin immunoprecipitation sequencing (ChIP-seq) method, Matthew Lorincz (University of British Columbia, Canada) reported that class I and class II long terminal repeat (LTR) retrotransposons are marked with ...
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Genome-scale reconstruction of the sigma factor network in Escherichia coli: topology and functional states
... The immunoprecipitation (IP) DNA and mock-IP DNA were hybridized onto high-resolution whole-genome tiling microarrays, which contained a total of 371,034 oligonucleotides with 50-bp probes overlapping by 25 bp on ...
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Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome wide promoter microarrays
... chromatin immunoprecipitation promoter microarray chip analysis (ChIP-chip) study summarizing gene promoters bound by ...before immunoprecipitation to show equal input ...
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Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
... The most widely used procedure to examine histone mod- ifications is Chromatin Immunoprecipitation (ChIP), a technique first established for cultured Drosophila cells [6]. In short, ChIP relies on antibodies to ...
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Respiratory syncytial virus proteins: identification by immunoprecipitation.
... Treatment of the infected cells with 0.15 M NaCl before labeling suppressed host cell protein synthesis and allowed clearer visualization of the five viral proteins by polyacrylamide gel[r] ...
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