coculture system
Matrix metalloproteinase 3–dependent generation of a macrophage chemoattractant in a model of herniated disc resorption
... loss of proteoglycan content and wet weight in the coculture system. The most straightforward interpre- tation of this result is that MMP-3 is required for pro- teolytic degradation of aggrecan, the major ...
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PubMedCentral-PMC5107669.pdf
... Bone infection, also called osteomyelitis, can result when bacteria invade a bone. Treatment of osteomyelitis usually requires surgical debridement and prolonged antimicrobial therapy. The rising incidence of infection ...
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<strong><em>Mycobacterium marinum:</em> a potential immunotherapy for <em>Mycobacterium tuberculosis</em> infection</strong>
... cell–bacillus coculture system in ...the coculture system and host immune responses including multinuclear giant cell formation by Wright–Giemsa modified staining, macrophage polarization by ...
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Molecular Umbrellas: a Novel Class of Candidate Topical Microbicides To Prevent Human Immunodeficiency Virus and Herpes Simplex Virus Infections
... When tissue explants were exposed to virus in the presence of 100 g/ml Spm8CHAS, amplification of infection by PM-1 cells in the coculture system was completely inhibited.. Spm8CHAS tar[r] ...
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Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
... Figure 6A shows clearly that there was a significant difference in uptake of LTVSPWY-PEG-CS-modified magnetic nanoparticles between SKOV-3 and A549 cells in the coculture system. Uptake of LTVSPWY-PEG-CS- ...
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Amoebal Coculture of “Mycobacterium massiliense” sp nov from the Sputum of a Patient with Hemoptoic Pneumonia
... A nonphotochromogenic, rapidly growing Mycobacterium strain was isolated in pure culture from the sputum and the bronchoalveolar fluid of a patient with hemoptoic pneumonia by using axenic media and an amoebal ...
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The metastatic infiltration at the metastasis/brain parenchyma- interface is very heterogeneous and has a significant impact on survival in a prospective study
... The current approach to brain metastases resection is macroscopic removal of metastasis until reaching the glial pseudo-capsule (gross total resection (GTR)). However, autopsy studies demonstrated infiltrating metastatic ...
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Cardiac-mimetic cell-culture system for direct cardiac reprogramming
... culture system can provide interactions with cardiomyocytes and electric cue to ...The system is equipped with a nano-thin, nanoporous, and the highly porous membrane, which can facilitate direct ...
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Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol
... a coculture system strategy comprised of two populations of Escherichia coli strains, each with a partial and complementary section of the pathway leading to the biosynthesis of the stilbene ...the ...
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Silencing tumor necrosis factor-alpha in vitro from small interfering RNA-decorated titanium nanotube array can facilitate osteogenic differentiation of mesenchymal stem cells
... In the meanwhile, the target of drugs silencing TNF- α and bone formation cells are different. Specifically, the TNF- α is secreted by activated monocytes while the bone formation relies on the osteogenic differentiation ...
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Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol
... As shown in Fig. 3d, MA production with the new coculture system of P5.2:BC was improved at all inoc- ulation ratios. At the optimal inoculation ratio of 1:1, MA concentrations reached 1016 mg/L. Moreover, ...
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Matrix metalloproteinase 7–dependent release of tumor necrosis factor α in a model of herniated disc resorption
... Disc/macrophage cocultures. The intervertebral disc/peri- toneal macrophage coculture system was described pre- viously (13). Briefly, activated murine macrophages were obtained by intraperitoneal ...
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Dual and Triple Epithelial Coculture Model Systems with Donor-Derived Microbiota and THP-1 Macrophages To Mimic Host-Microbe Interactions in the Human Sinonasal Cavities
... triple coculture system by inclusion of healthy donor-derived ...triple coculture setup. Similar to the dual coculture setup, coculture with ...triple coculture, now including ...
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Entamoeba histolytica trophozoites induce an inflammatory cytokine response by cultured human cells through the paracrine action of cytolytically released interleukin 1 alpha
... trophozoites. Coculture of human epithelial and stromal cells and cell lines with trophozoites is shown to increase expression and secretion of an array of chemoattractant and proinflammatory cytokines, including ...
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Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
... cells, changes in neutrophil transcript levels were greatest at 6 and 18 h of coculture with IAV. Each of the viruses caused upregulation of transcripts encoding immune response molecules (Fig. 4; Table S1). In ...
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Modification by Suppressor Cells and Serum Factors of the Cell Mediated Immune Response in Experimental Pyelonephritis
... Conversely, in coculture experiments, a suppressor cell present in the splenic lymphocyte population of pyelonephritic animals was shown to be capable of suppressing the mitogenic respon[r] ...
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“THE STUDY OF RELEVANCE OF MICROBIAL COCULTURE FERMENTATIONS IN BIOTECHNOLOGY” by Vaibhav Changediya, India.
... a coculture fermentation process consisting of Gluconacetobacter xylinus and Lactobacillus ...by coculture fermentation process involving Cellulomonas cellulans and Agrobacterium ...of coculture ...
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Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium
... Abstract: In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and ...
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Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development
... co-culture system might be higher than that of the medium alone or ...co-culture system might have a reduced toxic effect as compared with medium alone or vehicle ...
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Adaptation of Amoeba Plate Test To Recover Legionella Strains from Clinical Samples
... Patients and samples. This study prospectively included all respiratory specimens that were collected in February 2015 to September 2015 by the National Reference Centre for Legionella, as part of routine LD patient ...
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