Collagen gel-contraction
Effect of budesonide on fibroblast-mediated collagen gel contraction and degradation
... Conditioned media (2 mL) collected from the gel contrac- tion assay was concentrated by ethanol precipitation and resuspended in distilled H 2 O (50 µ L). The samples were then added to electrophoresis sample ...
9
Osteopontin is produced by rat cardiac fibroblasts and mediates A(II) induced DNA synthesis and collagen gel contraction
... antibody (20–100 mg/ml kindly provided by Thomas Borg, University of South Carolina) or nonimmune rabbit IgG (20–100 mg/ml) were preincubated for 30 min at 378C with cardiac fibroblasts before their incorporation into ...
11
Mechatronic devices for the investigation of fibroblast populated collagen gel contraction and the effects of mechanical loading.
... quiescent fibrocyte situated in the connective tissue (Grinnell 1994). When a wound is created, damage occurs to the connective tissue and blood vessels, resulting in the release of plasma proteins and blood cells into ...
193
TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels
... processes. Contraction of three-dimensional collagen gels is a model of wound healing and ...of contraction, TGF- β 1 and fetal calf serum (FCS) were evaluated for their effect on fibroblast ...
12
Triamcinolone Acetonide affects TGF β signaling regulation of fibrosis in idiopathic carpal tunnel syndrome
... in collagen gel contraction were consistent with those of previous studies: there was an in- creased cell-seeded gel contraction rate, stiffness and ten- sile strength in gels with ...
9
Platelets stimulate fibroblast-mediated contraction of collagen gels
... Neutralisation of human PDGF and TGF- β bioactivity Neutralising antibodies to PDGF and TGF-β were used to evaluate the relative contribution of these two agents to fibroblast-mediated collagen gel ...
10
Pirfenidone and nintedanib modulate properties of fibroblasts and myofibroblasts in idiopathic pulmonary fibrosis
... α-SMA, collagen gel contraction properties, invasion capabilities and myofibroblastic-like ultrastruc- tural features of cultured stromal cells derived from either healthy lung or from the lungs of ...
12
<p>The Pro-Fibrotic Behavior of Human Tenon’s Capsule Fibroblasts in Medically Treated Glaucoma Patients</p>
... Based on the presented data, we know that, in compar- ison to HTCFs isolated from patients not treated for glau- coma, HTCFs isolated from patients treated medically for glaucoma had an increased expression of pro- fi ...
12
Collagen matrix stiffness influences on fibroblast contraction force
... cellularised collagen gels is modified from the method of Bell et al ...the contraction of cells embedded in 3D matrices is relatively straight forward using the conventional collagen gel ...
15
A novel collagen gel based measurement technique for quantitation of cell contraction force
... cellular contraction force in vitro using a collagen gel-based sensing technique to demonstrate cell contraction force changes in fibroblasts treated with the agonist histamine, which ...
24
Enhanced Dupuytren's disease fibroblast populated collagen lattice contraction is independent of endogenous active TGF β2
... on collagen contraction in vitro using a three-dimensional fibroblast populated collagen lattice (FPCL) contraction assay and DD patient-matched disease and control pri- mary cell ...elevated ...
8
Development of a Platform for Studying 3D Astrocyte Mechanobiology: Compression of Astrocytes in Collagen Gels
... control and compressed collagen type I gel discs normalized to 0 hour static controls at.. “Horizontal” refers to cell alignment averaged from 4.[r] ...
29
Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats
... Several significantly changed groups were common to both muscles, including lipid metabolism, carbohydrate metabolism, muscle contraction, ion transport and collagen, although the number[r] ...
23
EFFECT OF COLLAGEN I GEL ON APOPTOSIS OF RAT HEPATIC STELLATE CELLS
... HSC are located in the space of Disse between endo- thelial cells and parenchymal cells and their main function is the uptake and storage of vitamin A and other retinoids. As a result of liver injury HSC loose ...
7
Control of fibroblast-mediated collagen contaction: Importance and mechanism of cell attachment in the contraction process
... and collagen (Sarkissian and Lafyatis, 1998), by increasing synthesis and decreasing breakdown (Desmouliere and Gabbiani, 1994; Reed et ...control collagen synthesis (Desmouliere, ...tissue ...
261
OCULAR DRUG DELIVERY SYSTEM: AN OVERVIEW
... Sol to Gel Systems: In 1980s a new concept producing a gel in situ was recommended by researchers. This concept of drug delivery to eye is widely used because it increases the viscosity and reduced the ...
7
Cellular and molecular aspects of articular cartilage resurfacing
... the collagen gel matrix undergo a change to a fibroblast-like morphology after 1-2 days in vitro, but after 5-6 days in culture they adopted a rounded cell shape, which was retained for up to 3 ...a ...
262
A method to investigate the anti-metabolic activity of anti-cancer agents on ovarian cancer cells cultured in a 96-well high throughput format
... containing collagen proteins. Thereby the ability of cells to grow in a three dimension (3D) following invasion is provided. Many studies have reported recently that cancer cells cultured in cell monolayer (2D) ...
12
In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats
... CMLA revealed remarkable wound healing properties. The histology results on day 15 after wound creation revealed less scarring of the wound area. The granulation tissue contained fewer inflammatory cells, as well as more ...
12
An overview of advanced biocompatible and biomimetic materials for creation of replacement structures in the musculoskeletal systems: focusing on cartilage tissue engineering
... Chitosan, as an antimicrobial polymer, which is de- rived from chitin, is a linear polysaccharide. The compo- nents of chitosan are glucosamine and N-acetyl- glucosamine. This type of natural polymer due to its ex- ...
21