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Comparison of DNA extraction methods

Pathogen quantitation in complex matrices : a multi operator comparison of DNA extraction methods with a novel assessment of PCR inhibition

Pathogen quantitation in complex matrices : a multi operator comparison of DNA extraction methods with a novel assessment of PCR inhibition

... Article Title: Pathogen Quantitation in Complex Matrices: A Multi- Operator Comparison of DNA Extraction Methods with a Novel Assessment of PCR Inhibition1. Year of publication: 2011 [r] ...

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Comparison of dna extraction methods for detection of citrus huanglongbing in colombia

Comparison of dna extraction methods for detection of citrus huanglongbing in colombia

... Capture of Diaphorina citri psyllids Adult individuals were collected using entomological nets, while the nymphs of D. citri were directly taken from symptomatic trees in different phenological stages (vege- tative, ...

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Comparison of DNA-extraction Methods Suitable for PCR-based Applications to Identify Shrimp Species in Commercial Products

Comparison of DNA-extraction Methods Suitable for PCR-based Applications to Identify Shrimp Species in Commercial Products

... different methods of DNA extraction were performed and compared in order to have the better DNA quality and quantity from specific seafood matrix such as various types of shrimp ...acids ...

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A comparison of DNA extraction methods from hair shafts: mitochondrial DNA analysis using next generation sequencing and nuclear DNA analysis using InnoQuant DNA quantification kit and InnoTyper 21 DNA typing kit

A comparison of DNA extraction methods from hair shafts: mitochondrial DNA analysis using next generation sequencing and nuclear DNA analysis using InnoQuant DNA quantification kit and InnoTyper 21 DNA typing kit

... Mitochondrial DNA Analysis – Discussion Part I Amplification success and amplicon concentration of each sample appeared to be more dependent on the hair sample than on the extraction ...combined ...

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Comparison of Different DNA Extraction Methods for Forensic Samples

Comparison of Different DNA Extraction Methods for Forensic Samples

... Abstract DNA analyses are important for different types of research in biomedical and forensic science and DNA isolation is an often required ...different DNA isolation kits are constantly becoming ...

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Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR

Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR

... extracted DNA was subjected to qPCR in duplicate, and extractions were performed in duplicate or ...fungal DNA detected with each DNA extraction method for each experimental system were ...

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Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize

Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize

... of DNA [5, 7]. Several methods to isolate DNA from plant tissues are available; however, these methods produce either small amounts or DNA of inconsistent ...the DNA ...

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Comparison of Total DNA Extraction Methods for Microbial Community Form Polluted Soil

Comparison of Total DNA Extraction Methods for Microbial Community Form Polluted Soil

... Abstract DNA isolation represents the basic and probably the most important step in molecular biology for microbial strains, and even more, for microbial community ...for DNA microbial community isolation, ...

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Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food

Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food

... different DNA extraction protocols for GMO detection through ...three methods tested, the DNeasy mericon food kit DNA extraction method obtained higher proportion of amplified ...

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Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen

Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen

... The DNA samples in both groups were precipitated, using 50 µl of 3 M sodium acetate (NaAc 300 mM, pH ...each DNA pellets was washed with cold (- 20 °C) 70% ethanol (250 ...the DNA pellet was dried. ...

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Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae  in Endarterectomy Specimens

Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae in Endarterectomy Specimens

... The overall detection rate (9%) and the maximum concor- dant positive rate (25% for one carotid artery) for the ather- oma samples were lower than expected. Possible explanations for these rather unexpected findings are ...

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Comparison of DNA Extraction Methodsfor Human Oral Microbiome Research

Comparison of DNA Extraction Methodsfor Human Oral Microbiome Research

... 29. Willner D, Daly J, Whiley D, Grimwood K, Wainwright CE, et al. Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar ...

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Improving Molecular Detection of Fungal DNA in Formalin Fixed Paraffin Embedded Tissues: Comparison of Five Tissue DNA Extraction Methods Using Panfungal PCR

Improving Molecular Detection of Fungal DNA in Formalin Fixed Paraffin Embedded Tissues: Comparison of Five Tissue DNA Extraction Methods Using Panfungal PCR

... intact DNA that is needed for amplification, since the longer amplicons are more difficult to achieve when DNA is highly fragmented or cross-linked, ...short DNA targets, such as the ⬃ 300-bp D2 ...

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Human skeletal remains: development of DNA extraction and typing methods

Human skeletal remains: development of DNA extraction and typing methods

... for DNA extraction from bones, verifying which kind of bone yields the best quality of DNA and evaluating different characteristics such as preservation, place of conservation and age of skeletal ...

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Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples

Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples

... and Methods Resource Population and Sample Collection At first, 11 blood samples were taken from each of 16 ewes of the Chios dairy breed raised in an experimental ...the DNA extraction ...

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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium

Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium

... optimal methods. For each technique, we tested two DNA extraction methods, several annealing temperatures for nested PCR reactions targeting the 18S, SSU rRNA (small subunit ribosomal RNA), ...

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Comparison of different protocols for the extraction of microbial DNA from reef corals

Comparison of different protocols for the extraction of microbial DNA from reef corals

... different DNA extraction methods on ...short DNA kit protocol) was the most efficient with respect to the amount of DNA that was obtained from the ...The DNA concentrations ...

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Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels

Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels

... environmental DNA (eDNA), originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non-invasive ...

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Genomic DNA from rat blood: A comparison of two extraction methods

Genomic DNA from rat blood: A comparison of two extraction methods

... isolated DNA, while agarose gel electrophoresis was carried out to evaluate DNA ...the extraction method has significantly influenced the quantity and purity of isolated ...weight DNA with ...

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DNA Extraction-Comparison of Methodologies

DNA Extraction-Comparison of Methodologies

... quality DNA is a prerequisite for all experiments of DNA ...plant DNA extraction protocols comprise of the basic steps of disruption of the cell wall, cell membrane and nuclear membrane to ...

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