Competitive ELISA
Development of competitive ELISA for neosporosis by employing immunoproteomics
... with these flaws, surface membrane antigens or serum capable of recognizing specific anti- gens have been used to increase both speci- ficity and sensitivity during serological tests (22,34). But development of these ...
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Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA
... Indirect competitive ELISA based on an anti- PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and ...
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Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum
... In intra-assay, five replicate samples containing ART at six different concentrations of 0.1, 0.3, 1.0, 3.0, 10, and 30 ng/ml were prepared and amounts of the ART in each sample were estimated using the described com- ...
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Generation of mAbs to foot–and–mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis
... and ELISA may detect different antibody ...virion ELISA reactivities and neutralization titers, suggesting a strong influence of antibody subset composition of individual ...
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One-step indirect competitive ELISA development for dexamethasone determination in chicken
... Dexamethasone (DEX) is a potent glucocorticoid that was widely employed in livestock production. In the present study, a one-step indirect competitive enzyme-linked immunosorbentassay (icELISA) was developed for ...
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The Multimerization of Hantavirus Nucleocapsid Protein Depends on Type-Specific Epitopes
... using competitive ELISA. Fig- ure 2D shows the schema for competitive ELISA and illus- trates the binding regions of the capture/detector ...
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Effective authentication of Placenta Hominis
... Methods: Ten samples claimed as Placenta Hominis were collected from herbal shops in China, Hong Kong and Taiwan. Species‑specific diagnostic primers for human, cow, deer and sheep were designed for PCR amplification and ...
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Analysis of Antibody Responses to Protective Antigen-Based Anthrax Vaccines through Use of Competitive Assays
... complete competition of human IgG antibodies could not be obtained using soluble PA83 as the competitor, even at high concentrations, whereas when combined with either rabbit or NHP sera, PA83 at the same concentration ...
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Identification of an immunodominant linear neutralization domain on the S2 portion of the murine coronavirus spike glycoprotein and evidence that it forms part of complex tridimensional structure.
... To elucidate the antigenic structure of the protein, a panel of monoclonal antibodies was studied by competitive ELISA, and their reactivities were assayed against fragments of the murin[r] ...
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Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients
... available competitive ELISA kit (Peninsula Laboratories International, San Carlos, CA, USA) was used for hepcidin ...sandwich ELISA kit (Roche Diag- nostics, ...
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Detection of Noncarboxymethyllysine and Carboxymethyllysine Advanced Glycation End Products (AGE) in Serum of Diabetic Patients
... Determination of CML and Non-CML AGE in Type 2 Diabetic Patients by Competitive ELISA. Type 2 diabetic patients without uremia were[r] ...
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Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.
... A competitive enzyme-linked immunosorbent assay (ELISA) using an anti-LPS monoclonal antibody improved the sensitiv- ity of the diagnosis but failed to differentiate vaccinated from infected animals ...in ...
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Cathepsin-S degraded decorin are elevated in fibrotic lung disorders – development and biological validation of a new serum biomarker
... The present study describes the development and bio- logical validation of a technically robust competitive ELISA assay quantifying a Cat-S degraded fragment of decorin in serum. The mai[r] ...
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Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content
... The plasmid construct pQE70 fra a 1.02 generated in a previous study [16,22] was used for transformation in the expression strain Escherichia coli (E. coli) BL21 (DE3) pLysS. Recombinant Fra a 1.02 was expressed as a ...
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Fast detection of 2,4,6-trinitrotoluene (TNT) at ppt level by an immunosensor based on kinetic competition
... Figure S17 Comparison of the clone A1.1.1 and EW75C in indirect competitive ELISA. The detection limit for the clone A1.1.1 was determined to be 0.17 nM or 39 ng/L, the IC50 at 1.2 nM or 270 ng/L, and the ...
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Serological Evidence of Blutongue Virus Serotype 9 Circulation in Albania Cattle during 2012-2013
... Materials and methods. In total, 1348 bovine sera blood samples from serum bank were tested by serological competitive ELISA method. Identified cluster positive farms were identified and were follow on at ...
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High performance liquid chromatography and Enzyme Linked Immunosorbent Assay techniques for detection and quantification of aflatoxin B1 in feed samples: a comparative study
... and ELISA techniques have been utilized for a number of years [14], the avail- able information on comparison of the techniques is ...a competitive ELISA method was standardized, validated and its ...
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<p>Diagnosing bluetongue virus in domestic ruminants: current perspectives</p>
... Abstract:This review provides an overview of current and potential new diagnostic techniques against bluetongue virus (BTV), an Orbivirus transmitted by arthropods that affects ruminants. Bluetongue is a disease ...
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Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt
... The serological tests, including the indirect fluorescent antibody test (IFAT) and the enzyme-linked immune- sorbent assay (ELISA) are capable of detecting anti- bodies in carrier animals; therefore they are often ...
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Interference of Vaccination against Bluetongue Virus Serotypes 1 and 8 with Serological Diagnosis of Small-Ruminant Lentivirus Infection
... indirect ELISA (C-ELISA) and a competitive ELISA (L-ELISA) mea- suring the displacement of an anti-gp135 monoclonal ...SRLV ELISA following BTV vaccination are elicited from ...
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