Complex assembly and disassembly in nuclear translocation
Sequential SNARE disassembly and GATE 16 GOS 28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion
... 2 a, condition 2]) or generated in the presence of NEM but treated with wt NSF before fusion (Fig. 2 a, condition 8). This implies that MGFs only reform cisternae if they have been exposed to wt NSF at one stage of the ...
14
Functional characterization of the nucleoporins Nup50 and Nup155 in postmitotic nuclear pore complex assembly
... CDK1-cyclinB complex, Aurora B kinase, proteins of the NIMA family, and others (for review see (Guttinger et ...2009)). Nuclear disassembly is required in open mitosis in order for the mitotic ...
172
COPI-Mediated Nuclear Translocation of EGFRvIII Promotes STAT3 Phosphorylation and PKM2 Nuclear Localization
... the nuclear translocation of EGFRvIII, we performed immunoblotting analysis, in which BFA was administered to inhibit the activation of ARF1- guanine exchange factors, resulting in the disassembly of ...
13
Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells
... The most interesting change was on capsid residue N57, which directly interacts with the FG repeat of Nup153. Infection of nondividing cells by HIV-1 viruses with these capsid mutations was found to be defective for ...
29
The assembly and disassembly of clathrin cages
... 4.1.5 Measuring clathrin cage and vesicle size In vitro the measurement of clathrin cages has been primarily through the use of negative stain or cryo-transmission electron microscopy (EM). Being a large complex ...
232
Nup153 Recruits the Nup107-160 Complex to the Inner Nuclear Membrane for Interphasic Nuclear Pore Complex Assembly
... INTRODUCTION Nuclear pore complexes (NPCs) are gatekeepers of the ...and nuclear interior and enable tightly controlled transport between these compartments (Wente and Rout, ...2010). Nuclear import ...
13
Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection
... the nuclear pore complex (NPC), which regulates the passage of proteins, nucleic acids, and solutes through the nuclear envelope, require virus capsid uncoating before viral DNA can access the ...
72
Kinesin-1-Mediated Capsid Disassembly and Disruption of the Nuclear Pore Complex Promote Virus Infection
... and Nuclear Envelope Permeability So far, the data indicated that the kinesin-1 motor protein binds to the viral capsid and the NPC, releases Nups from the nuclear envelope during capsid disruption, and ...
14
The regulation of cilium assembly and disassembly in development and disease
... cilium disassembly, Plk1 and Nek2 activate the kinesins Kif2a and Kif24, respectively, which are required for the depolymerization of ...Cilium disassembly also requires the modulation of IFT transport: ...
13
The Karyopherin Kap95 Regulates Nuclear Pore Complex Assembly into Intact Nuclear Envelopes In Vivo D
... NPC assembly and results in the cytoplasmic accumulation of distinct Nup containing vesicles (Ryan et ...NPC assembly factors to the ONM where RanGTP is re- quired for their fusion to the ...NPC ...
13
Assembly and Translocation of Papillomavirus Capsid Proteins
... VP1-VP2/VP3 complex. In this study, we describe independent nuclear translocation of the L1 major protein and the L2 minor capsid protein of human papillomavirus type 33 by several ...and ...
6
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
... Figure 4. Ribbon Model of the Crystal Structure of Bovine Arp2/3 Complex The inactive structure from the crystal has the actin-related proteins, Arp2 and Arp3, separated too far to initiate a new filament. The ...
13
Insight into Structure and Assembly of the Nuclear Pore Complex by Utilizing the Genome of a Eukaryotic Thermophile
... This assembly, which potentially spans from the pore membrane to the FG transport channel, consists of Nup192 and Nup170, bridged by Nic96 and ...this complex, we have identified nucleoporin interactions ...
13
Cytomegalovirus Assembly Protein Precursor and Proteinase Precursor Contain Two Nuclear Localization Signals That Mediate Their Own Nuclear Translocation and That of the Major Capsid Protein
... pAP-MCP complex formation, to enable MCP nuclear trans- ...(HSV-1), nuclear transport of MCP requires (63) or is enhanced by (42) coexpression with their respective pAP, both of which contain an NLS1 ...
11
DNA damage induces nuclear translocation of parkin
... immune complex buffer (Upstate), twice with a high salt wash buffer, once with a LiCl wash buffer (Upstate), and twice in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ...
9
Roles of the Nuclear Lamina in Stable Nuclear Association and Assembly of a Herpesviral Transactivator Complex on Viral Immediate Early Genes
... the nuclear periphery are silenced by ...the nuclear lamina and that this localization is associated with reduced heterochromatin on the viral genome and increased viral immediate-early (IE) gene ...the ...
11
Effective assembly of fimbriae in Escherichia coli depends on the translocation assembly module nanomachine
... molecular assembly platform called “the usher” located in the outer membrane 6,7 ...-barrel assembly machine (BAM) complex 8,9 ...usher assembly is crucially dependent on another nanomachine, ...
25
CiteSeerX — JCB Article Nuclear RNP complex assembly initiates cytoplasmic RNA localization
... junction complex (Hachet and Ephrussi, 2001; Mohr et ...earlier nuclear events in RNA biogenesis such as tran- scription and ...the nuclear and cytoplasmic steps in the vegetal RNA localization ...
9
Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex
... The nuclear pore complex (NPC) is a protein assembly that contains several distinct ...hetero-oligomeric complex, that also contains Nup160, Nup133, Nup96, and the mammalian homolog of yeast ...
5
Assembly and mechanism of bacterial twin arginine translocation systems
... the translocation mechanism propose the coalescence of a substrate-binding TatABC complex with a separate TatA ...the translocation pore, and the size var- iation of TatA has been linked to the ...
212