Conditioned media (CM)
<p>Antitumor effects of conditioned media of human fetal dermal mesenchymal stem cells on melanoma cells</p>
... 1 CM-FDMSC inhibits tumor development in ...of CM-FDMSC (0.5× CM, 1× CM, 2× CM) or control medium ...Abbreviations: CM, conditioned media; CON, ...
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Conditioned media from adipose stromal cells limit lipopolysaccharide induced lung injury, endothelial hyperpermeability and apoptosis
... ; media was collected 24 h later, and kept frozen until future ...analysis. Conditioned media for animal injections had to be FBS-free, and there- fore was generated from mASC using basal EBM2 with ...
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Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.
... (4T1) conditioned media (CM) yielded the greatest induction of Cdc42EP3 protein expression (Figures 7F and ...4T1 CM for 48 hr trig- gered F-actin and septin rearrangements (Figures S7B and ...
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Exosomes from IL 1β stimulated synovial fibroblasts induce osteoarthritic changes in articular chondrocytes
... SFB conditioned media (2 mL/well) were collected and cells were washed with PBS, and total RNA was isolated with TRIzol reagent (Life ...SFB-conditioned media (2 mL/well) were centrifuged for ...
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Allogenic adipose-derived stem cell therapy overcomes ischemia-induced microvessel rarefaction in the myocardium: systems biology study
... infusion); conditioned media (CM; intravenous infusion); ASCs + CM; or PBS/placebo ...and CM was performed to characterize the ASC protein ...
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Therapeutic Potential of Lung Spheroid Cell-Secreted Factors in Rodent Models of Pulmonary Fibrosis.
... of CM-mediated regeneration may relate to the EXOs, MMP2 activity, and a host of proteins found in the ...CM. Conditioned media and EXOs are considerably less immunogenic then their parent ...
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Regulation of alveolar macrophage transforming growth factor beta secretion by corticosteroids in bleomycin induced pulmonary inflammation in the rat
... Alveolar and Splenic Macrophage Secretion of TGF-13 after Intratracheal Bleomycin or Normal Saline Administration TGF-fl pg/ I6 macrophages per 24 h±SE* Source of conditioned media CM.. [r] ...
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Stem cells from human exfoliated deciduous teeth as an alternative cell source in bio-root regeneration
... CM: conditioned media; DFCs: dental follicle cells; DFCSs: dental follicle cell sheets; DPSCs: dental pulp stem cells; ECM: extracellular matrix; GMSCs: gingival mesenchymal stem cell[r] ...
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Breast cancer cells promote a notch dependent mesenchymal phenotype in endothelial cells participating to a pro tumoral niche
... Abbreviations BCCs: Breast cancer cells; BCSCs: Breast cancer stem cells; BMP: Bone morphogenic protein; CAFs: Cancer-associated fibroblasts; CM: Conditioned media; ECs E4-ECs: Endotheli[r] ...
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Interleukin 34 produced by human fibroblast like synovial cells in rheumatoid arthritis supports osteoclastogenesis
... albumin; CM: conditioned media; DAS28: disease activity score 28; DMARDs: disease-modifying antirheumatic drugs; ERK: extracellular signal-regulated protein kinase; FLS: fibroblast-like synovial ...
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Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration
... and conditioned media ...to conditioned media (CM) from these cell lines, increased the lipolytic rate of 3T3-L1 adipocytes, as determined by non-esteri- fied fatty acid (NEFA) and ...
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Herbal compound “Songyou Yin” attenuates hepatoma cell invasiveness and metastasis through downregulation of cytokines secreted by activated hepatic stellate cells
... and conditioned media (CM-SYY and CM-nSYY, respectively) were ...SYY, CM-SYY, and CM-nSYY, designated McA-SYY, McA-SYYCM and ...in conditioned media were tested ...
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Oncolytic reovirus as a combined antiviral and anti-tumour agent for the treatment of liver cancer.
... resultant conditioned culture supernatants should exert antiviral ...Filtered conditioned media (CM) were prepared follow- ing control, or Reo (reovirus-CM (RCM)) stimulation of mixed ...
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Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines
... marrow; CM: Conditioned media; CXCR4: C-X-C chemokine receptor type 4; C: Cytoplasmatic; Day: D; Dc: Decoy; ER: Estrogen-R; FBS: Fetal bovine serum; HER-2: Epidermal growth factor receptor 2; M: ...
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Regulation of the bone vascular network is sexually dimorphic
... WT CM were observed (Figure ...OBVEGFKO conditioned media versus WT but reduced following treatment with female OBVEGFKO media ...OBVEGFKO CM versus WT; which may contribute to the ...
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Ell3 functions as a critical decision maker at the crossroad between stem cell senescence and apoptosis
... Abbreviations: C, control plasmid; Ell3 OE, Ell3-expressing plasmid; C-CM, conditioned media prepared from the culture media of ADSCs transfected with the control plasmid; OE-CM, conditi[r] ...
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Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response
... the conditioned media of ...tioned media was transferred to the apical surface of vir- gin epithelia while the basolaterally conditioned media was transferred to the basolateral aspect ...
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The synthetic triterpenoid CDDO-methyl ester modulates microglial activities, inhibits TNF production, and provides dopaminergic neuroprotection
... or conditioned media from LPS and CDDO-Me treated BV2 microglial ...day conditioned media from BV2 cultures was removed and centrifuged at 1200 × g for 4 min before addition to DCFDA-loaded, ...
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Activation of Astrocytes in Vitro by Macrophages Polarized with Keratin Biomaterial Treatment
... was only for 24 hours due to the need to change the media every third day. This may suggest some res- ponses are being underestimated because there may be some factors that are present in lower concentrations ...
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Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing
... with conditioned medium (CM) pro- duced by UCX® grown in classical two-dimensional mono- layer cultures, have demonstrated the potential for promoting cutaneous wound healing ...
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