Conserved active site residues
Mechanistic studies to determine the catalytic roles of active site residues in phosphite dehydrogenase
... methionine residues are most commonly associated with hydrophobic interactions and steric ...the conserved Met53 appears to interact with an oxygen atom on the sulfite competitive ...
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Importance of Neuraminidase Active-Site Residues to the Neuraminidase Inhibitor Resistance of Influenza Viruses
... charged conserved NA residues that directly interact with NAIs and have not been reported to confer resistance to ...NA residues we studied are under strong a selective pres- sure of the drug and are ...
9
Identification of Active-Site Amino Acid Residues in the Chiba Virus 3C-Like Protease
... acid residues played critical roles in the prote- olysis in the NLV 3C-like ...acid residues as well as putative nucleo- phile Cys139 of the Chiba virus 3C-like protease to identify ...
10
Mutations in a Conserved Residue in the Influenza Virus Neuraminidase Active Site Decreases Sensitivity to Neu5Ac2en-Derived Inhibitors
... in residues directly associated with re- ceptor binding ...altered residues in these mutants were not directly involved in receptor binding, the ju2 Gly143Glu mutation is close to residues 134 ...
7
Identification of Conserved Residues Contributing to the Activities of Adenovirus DNA Polymerase
... of conserved residues at the active site ...These residues are conserved among RB69 DNA Pol, 29 DNA Pol, human DNA Pol ␣, herpes simplex virus DNA Pol, and adenovirus DNA Pol, ...
9
Important Role for Phylogenetically Invariant PP2Acα Active Site and C-Terminal Residues Revealed by Mutational Analysis in Saccharomyces cerevisiae
... mu- conserved PP2Ac␣ active site and C-terminal residues that are invariant between ...identify active site residues important for enzyme ...
9
Effects of Mutations in Residues near the Active Site of Human Immunodeficiency Virus Type 1 Integrase on Specific Enzyme-Substrate Interactions
... acid residues, the glutamic acid residue of the DD(35)E motif of HIV-1 integrase was rela- tively intolerant of ...other active-site mutants, IN(E152D) dis- played a marked indifference to mutations ...
10
Invariant residue-a residue that is always conserved. It is assumed that these residues are essential to the structure or function of the protein.
... 4) Can have buried water molecules in the interior of the protein A long polypeptide strand folds into domains: each domain typically contains 100-150 continuous amino acids The domains are separated by a section of ...
5
The copper active site of CBM33 polysaccharide oxygenases
... ed conserved tyrosine residues on the α -helical L2 loop in polysaccharide oxygenases and also on residues near the copper active site (Figure ...The residues are not only in di ...
9
Genetic Analyses of Conserved Residues in the Carboxyl-Terminal Domain of Human Immunodeficiency Virus Type 1 Integrase
... CTD residues were targeted by mutagenesis, generating 24 mutant ...binding, residues were tar- geted due to their degree of sequence conservation among a large collection of HIV-1/SIV cpz ...
13
Investigation into the Chemical Modification of Active Site Residues of an Aldolase Enzyme
... 14 1.3.1 Schultz’s methodology for the incorporation of unnatural amino acids Over the past two decades Schultz and co-workers have developed methods to expand the genetic code. The method involves manipulating one of ...
151
Labeling of Specific Lysine Residues at the Active Site of Glutamine Synthetase*
... After this reductive methylation procedure, labeling of the enzyme with [14C]thiourea dioxide (25 mM, 24 h) was attempted. No incorporation of radiolabel was observed. This lea[r] ...
9
Identification of active-site residues in protease 3C of hepatitis A virus by site-directed mutagenesis.
... the active site, and another amino acid residue may be the third constituent of the putative catalytic triad, if the latter actually ...the active site ...acid residues were too dis- ...
7
of kinetically active residues
... blue ribbon). Sofosbuvir is green molecule inside the HCV RdRp catalytic core. SAGNM predictions are dark yellow atoms. Sofosbuvir is green molecule inside the 455. catalytic core. The[r] ...
28
Identification of the active-site residues of the L proteinase of foot-and-mouth disease virus.
... Molecular modeling. At present, neither X-ray crystallographic structures nor nuclear magnetic resonance models exist for L proteinase. Since no such models are currently available, we developed a theoretical model for L ...
7
Importance of Conserved Cysteine Residues in the Coronavirus Envelope Protein
... have conserved cysteine residues located on the carboxy side of the long hydrophobic domain, suggesting functional ...the conserved residues and the necessity of palmitoylation, three ...
11
Diverse Roles of Glycine Residues Conserved in Photoactive Yellow Proteins
... glycine residues was studied by alanine-scanning mutagenesis using photoactive yellow protein, a structural prototype of PER ARNT SIM domain proteins, as a ...these residues regulate the light-induced ...
9
Mutations in active-site residues of the uracil-DNA glycosylase encoded by vaccinia virus are incompatible with virus viability.
... Three active-site amino acids of the vaccinia virus uracil-DNA glycosylase were mutated (Asp-683Asn, Asn-1203Val, and His-1813Leu), producing proteins that were completely defective in uracil excision but ...
9
Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3
... for active site residues of the den- gue virus NS3 protease and therefore suggest that the residues selected in this study have probably a major function for substrate binding and ...the ...
8
Sleep-Active Neurons: Conserved Motors of Sleep
... ABSTRACT Sleep is crucial for survival and well-being. This behavioral and physiological state has been studied in all major genetically accessible model animals, including rodents, fish, flies, and worms. Genetic and ...
11