Construction of the molecular clone JC105B
Construction and Characterization of an Infectious Molecular Clone of Koala Retrovirus
... molecular clone. In this study, we constructed an infectious molecular clone, termed plasmid pKoRV522, of a KoRV isolate (strain Aki) from a koala reared in a Japanese ...the ...
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Construction of a molecular clone of ovine enzootic nasal tumor virus
... Initially we thought that the defect in Gag polyprotein processing could be due to the requirement for a host cell protein found only in sheep cells or alternatively, due to the presence a restriction factor in human ...
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Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice.
... This find- ing demonstrates that excision and replication as a linear molecule are dependent on both terminal sequences being present in the same molecule and that, in the absence of eit[r] ...
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Construction and Characterization of a Human T-Cell Lymphotropic Virus Type 3 Infectious Molecular Clone
... lecular clone is functional and produces infectious viral parti- ...this clone will be a unique and powerful tool that will allow us to investigate HTLV-3 protein expression and viral pathogenesis in ...
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Construction and Analysis of an Infectious Human Immunodeficiency Virus Type 1 Subtype C Molecular Clone
... the construction and bio- logical analysis of the first infectious proviral DNA molecular clone of HIV-1 subtype C from the African ...This clone is a chimeric molecule, because the majority ...
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Nucleotide sequence of the 5'-terminal palindrome of Aleutian mink disease parvovirus and construction of an infectious molecular clone.
... In situ molecular hybridization for detection of Aleutian mink disease parvovirus DNA by using strand-specific probes: identification of target cells for viral replication in cell cultur[r] ...
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Thymic Lesions in Cats Infected with a Pathogenic Molecular Clone or an ORF-A/2-Deficient Molecular Clone of Feline Immunodeficiency Virus
... Importantly, the JSY3⌬ORF-A/2-infected cat C5-1 pro- duced a virus isolate which was an exception to the replication- impaired ORF-A/2-deficient phenotype. Virus isolated from C5-1 was able to replicate to high levels in ...
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Grapevine Vein Clearing Virus: Epidemiological Patterns and Construction of a Clone
... estimated molecular clock will convert generations to calendar time, the discrete geographic coordinates of the sampled isolates will be fixed points in a continuous landscape, the resolution of phylogenetic ...
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Construction and Transposon Mutagenesis inEscherichia coli of a Full-Length Infectious Clone of Pseudorabies Virus, an Alphaherpesvirus
... The herpesviruses are a large group of viruses characterized, in part, by a double-stranded linear DNA genome ranging in size from approximately 80 to 250 kb. Representatives of this family cause recurrent infections in ...
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Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome
... In the study described here, the application of a mutagenesis system which was independent of viral replication allowed for the rapid production of a gE-negative mutant. The subsequent transfection into bovine cells ...
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The 5′ Untranslated Region of a Novel Infectious Molecular Clone of the Dicistrovirus Cricket Paralysis Virus Modulates Infection
... viral molecular mechanisms are hampered due to a lack of an estab- lished infectious ...the construction of the first infectious clone of the dicistrovirus, cricket paralysis virus ...CrPV ...
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Construction and characterization of an infectious clone of coxsackievirus A16
... first construction of an infec- tious cDNA clone of ...infectious clone con- tains the full-length cDNA of CVA16 flanked by a T7 promoter and a poly(A) tail at the 5’ and 3’ ends, ...cDNA ...
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Molecular and biological characterization of a neurovirulent molecular clone of simian immunodeficiency virus.
... the molecular determinants of neurovirulence, we constructed an infectious simian immunode- ficiency virus (SIV) molecular clone, SIV/17E-Fr, that contained the 3 * end of a neurovirulent strain of ...
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Construction and characterization of an infectious cDNA clone of coxsackievirus A 10
... Results: CA10 viruses were rescued from the constructed cDNA clone and reached a maximum titer of 10 8.125 TCID 50 /mL after one generation in RD cells. The virus exhibited similar physical and chemical properties ...
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Molecular Characterization of an Epidemic Clone of Panantibiotic Resistant Pseudomonas aeruginosa
... epidemic clone showed an overexpression of the chromosomal cephalosporinase AmpC combined with decreased expression of porin OprD and the absence of metallo-  -lactamase or extended-spectrum ...epidemic ...
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Molecular Epidemiology of Pseudomonas aeruginosa Colonization in a Burn Unit: Persistence of a Multidrug Resistant Clone and a Silver Sulfadiazine Resistant Clone
... The medical staff of the BWC concluded on 10 August that an outbreak was occurring, while retrospective epidemiological analysis demonstrated that on 3 July four patients were already colonized with the AFLP35 epidemic ...
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Molecular Characterization of Mycoplasma agalactiae Reveals the Presence of an Endemic Clone in Spain
... Endemic Clone in Spain Jaime Ariza-Miguel, David Rodríguez-Lázaro, Marta Hernández Instituto Tecnológico Agrario de Castilla y León (ITACyL), Consejería de Agricultura y Ganadería, Junta de Castilla y León, ...
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Development and Characterization of an In Vivo Pathogenic Molecular Clone of Equine Infectious Anemia Virus
... nonpathogenic molecular clone (19-2-6A) of equine infectious anemia virus (EIAV) was modified by substitution of a ...19-2-6A molecular clones were designated EIAV ...these molecular clones ...
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Multiple clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology
... immunity and anti-malarial drug use, and the historical- demographic processes of the parasite population. Com- piling the available data on the genetic diversity of P. vivax from different geographic regions higher ...
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Characterization of a Highly Pathogenic Molecular Clone of Feline Immunodeficiency Virus Clade C
... V2 sequences has an effect on the biological activity of Rev function is not certain. FIV-C36 was demonstrated to be infectious by conducting transfection and infection studies in vitro and in vivo. CrFK cells are ...
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