Construction of an Infectious Viral Clone 142
Construction and characterization of an infectious clone of coxsackievirus A16
... entire viral genome can serve as a template for the generation of infectious ...These infectious cDNA clones pro- vide a platform for the manipulation of viral genomes and they provide a ...
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Construction and Characterization of an Infectious Molecular Clone of Koala Retrovirus
... molecular clone. In this study, we constructed an infectious molecular clone, termed plasmid pKoRV522, of a KoRV isolate (strain Aki) from a koala reared in a Japanese ...in viral ...
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Construction and characterization of an infectious cDNA clone of coxsackievirus A 10
... Methods: The whole genome of CA10 derived from a clinical isolate was amplified into two fragments and ligated into a linearized plasmid vector in one step by In-Fusion Cloning. The obtained CA10 cDNA clones and plasmids ...
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Construction and Characterization of a Human T-Cell Lymphotropic Virus Type 3 Infectious Molecular Clone
... lecular clone is functional and produces infectious viral parti- ...the viral life cycles of both STLV-3 and HTLV-3 in a rabbit model (11) will now allow us to ascertain whether the 366-bp ...
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Construction and Analysis of an Infectious Human Immunodeficiency Virus Type 1 Subtype C Molecular Clone
... the construction and bio- logical analysis of the first infectious proviral DNA molecular clone of HIV-1 subtype C from the African ...This clone is a chimeric molecule, because the majority ...
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Construction and Transposon Mutagenesis inEscherichia coli of a Full-Length Infectious Clone of Pseudorabies Virus, an Alphaherpesvirus
... PFU/cell). Viral DNA recovered from the final infec- tion was examined by EcoRI digestion and compared to diges- tions of vBecker1 and PRV-Becker DNAs, which are not cut by ...the viral genomes are a more ...
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Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome
... of viral replication allowed for the rapid production of a gE-negative ...fectious clone demonstrated that the virus did not form visible plaques under agarose ...BoHV-1 infectious BAC clones ...
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Construction and characterization of an improved DNA launched infectious clone of duck hepatitis a virus type 1
... of viral RNA rescued system, including RNA-launched system [18], helper virus-launched system [19] and two DNA- launched systems based on cellular RNA polymerases I and II [20 – ...the infectious DHAV ...
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Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts.
... which infectious RNA can be transcribed have already been described for numerous other RNA viruses (1, 17, 18, 22, ...the construction of such an ‘‘infectious cDNA’’ clone for human astrovirus ...
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Nucleotide sequence of the 5'-terminal palindrome of Aleutian mink disease parvovirus and construction of an infectious molecular clone.
... In situ molecular hybridization for detection of Aleutian mink disease parvovirus DNA by using strand-specific probes: identification of target cells for viral replication in cell cultur[r] ...
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Construction of a full length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain
... the viral genome into a BAC system, as it allows stable maintenance the viral genome in ...the viral genome to be easily generated using various ...on viral growth in cell culture ...for ...
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An Infectious Clone of Woolly Monkey Hepatitis B Virus
... DISCUSSION Construction of a WM GGL ...from viral DNA purified from the serum of an infected woolly monkey ...full-length clone, since it was found to contain a number of nucleotide changes in ...
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Construction of a Severe Acute Respiratory Syndrome Coronavirus Infectious cDNA Clone and a Replicon To Study Coronavirus RNA Synthesis
... full-length infectious cDNA clone and a functional replicon of the severe acute respiratory syndrome coronavirus (SARS-CoV) Urbani strain as bacterial artificial chromosomes (BACs) is described in this ...
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Construction and sequencing of an infectious clone of the goose embryo adapted Muscovy duck parvovirus vaccine strain FZ91 30
... still infectious, as evidenced with successful viral rescue after plasmid transfection in 11- day-old embryonated goose ...of viral rescue ...
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Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks
... Results: All Nicotiana occidentalis plants inoculated with ≥ 0.2 μ g capped RNA transcripts from pPVS-H-FL- β developed symptoms on upper leaves, as observed with PVS-H00 inoculation. Similar levels of viral ...
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Rescue of a duck circovirus from an infectious DNA clone in ducklings
... an infectious viral clone We first generated two monomeric, full-length DuCV DNA clones, denoted IC-1 and IC-2, that were derived from the prototype Sichuan isolate GH01 ...each clone with ...
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Genome Sequence, Full-Length Infectious cDNA Clone, and Mapping of Viral Double-Stranded RNA Accumulation Determinant of Hypovirus CHV1-EP721
... of viral dsRNA accumulation was observed in European ...of viral dsRNA accumulation has not been assigned either to the nature of the host fungus or to the property of the ...of viral dsRNA accumu- ...
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Recovery of a Neurovirulent Human Coronavirus OC43 from an Infectious cDNA Clone
... Recovery, replication, and neurovirulence of recombinant HCoV-OC43. One of the major advantage provided by the BAC system is that the recovery of recombinant viral particles is very efficient and simple. This was ...
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Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
... or infectious, with a deletion of one or more viral genes due to the BAC vector ...multipurpose clone in research on HSV-1, we attempted to generate infectious HSV-BACs containing the full ...
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Construction of an Infectious cDNA Clone of Aichi Virus (a New Member of the Family Picornaviridae) and Mutational Analysis of a Stem-Loop Structure at the 5′ End of the Genome
... detecting viral RNA replica- tion, we constructed a mutant which contains an in-frame de- letion spanning from nt 1387 to 1848 within the VP0 coding region ...
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