control siRNA
Retrograde Transport from Early Endosomes to the trans-Golgi Network Enables Membrane Wrapping and Egress of Vaccinia Virus Virions
... a control siRNA or siRNA to STX6 or VPS52 and then infected for 18 h with a recombinant VACV in which the A4 core protein was fused to GFP, allowing detection of unwrapped and wrapped virions as FIG ...
15
Notch1 binds and induces degradation of Snail in hepatocellular carcinoma
... Notch1 siRNA than those transfected with control siRNA (Figure ...Snail siRNA-transfected Hep3B cells after treat- ment with H 2 O 2 ...
12
Original Article Knock-down of B7-H3 by small interfering RNA promotes gemcitabine-induced apoptosis in pancreatic cell line Patu8988t
... B7-H3 siRNA was been used in the following experimental ...negative control siRNA group, B7-H3 siRNA group, gemcitabine group, gemicitabine + negative control siRNA group and ...
9
Chromosome congression is promoted by CENP Q and CENP E dependent pathways
... in control and CENP-Q-siRNA-treated cells, ...with control siRNA and siRNA- resistant CENP-Q–eGFP vector (black), CENP-Q siRNA and siRNA- resistant CENP-Q–eGFP vector ...
15
Vascular Endothelial Growth Factor A Promotes Vaccinia Virus Entry into Host Cells via Activation of the Akt Pathway
... VV to MPVe-165 and MPVC cells. MPVe-165 or MPVC cells were infected with wild-type VV at an MOI of 10 PFU/cell for 1 h at 4°C to allow for virus attachment. Cells were then washed and harvested for analysis of the amount ...
10
Stimulation of translation by human Unr requires cold shock domains 2 and 4, and correlates with poly(A) binding protein interaction
... negative control siRNA-treated samples for each cell ...negative control siRNA-treated samples and Unr siRNA-treated ...no siRNA, siRNA against Unr (siUnr) or a negative ...
10
Original Article Silencing MAGE-C1/CT7 enhances the anticancer effects of bortezomib in multiple myeloma cell line
... in siRNA group (cells transfected with siRNA-MAGE-C1/CT7) significantly decreased and that of cells in siRNA+Bt group (cells transfected with siRNA-MAGE-C1/CT7 and treated with bortezomib) ...
11
Identifying and targeting determinants of melanoma cellular invasion
... GLIS3 siRNA transfected melanoma cells was inhibited following transplantation into the embryonic chicken neural tube compared to control siRNA transfected melanoma ...
17
Requirement for sphingosine 1–phosphate receptor 1 in tumor angiogenesis demonstrated by in vivo RNA interference
... 1 siRNA, but not a negative control siRNA, into established tumors inhibited the expression of S1P 1 polypeptide on neovessels while concomitantly ...
9
Human CDK18 promotes replication stress signaling and genome stability.
... to control cell cycle progression, we next assessed the cell cycle distribution of a CDK18-depleted cell ...non-targeting control siRNA (Figure ...
18
In vitro evaluation of endothelial exosomes as carriers for small interfering ribonucleic acid delivery
... nmol/mL siRNA against luciferase (siRNA[luc], silencer firefly luciferase siRNA), or nonsilencing control siRNA (siRNA[cont] Ambion; Thermo Fisher Scientific) on ice for 10 ...
8
Hsp27 participates in the maintenance of breast cancer stem cells through regulation of epithelial mesenchymal transition and nuclear factor κB
... specific siRNA, the population of ALDH+ cells in AS-B145 or AS-B244 cells was significantly decreased to ...negative control siRNA (Figure ...negative control siRNA or Hsp27 specific ...
13
Resveratrol Inhibits the TRIF-Dependent Pathway by Upregulating Sterile Alpha and Armadillo Motif Protein, Contributing to Anti-Inflammatory Effects after Respiratory Syncytial Virus Infection
... negative- control siRNA-treated ...and siRNA 3-1-treated had significantly increased lymphocyte numbers (P ⬍ ...and siRNA negative control ...
8
ARID3B: a Novel Regulator of the Kaposi's Sarcoma-Associated Herpesvirus Lytic Cycle
... to control DNA (am- plified from the RTA coding region) and a central G/C-rich repeat region (9F), we found that ARID3B preferentially bound to region 3F, which contains the A/T-rich region of KSHV oriLyt ...
13
RNAi mediated knock down of Dab and Numb attenuate Aβ levels via γ secretase mediated APP processing
... Dab siRNA or control siRNA, the cells were harvested and subjected to Western blot analyses with antibody ...Dab siRNA treatment did not alter endogenous levels of FL-APP as compared to con- ...
10
USP4 Positively Regulates RIG-I-Mediated Antiviral Response through Deubiquitination and Stabilization of RIG-I
... vector control and HA-Ub (WT), HA-Ub (K48), or HA-Ub (K63) plasmids were subjected to immunoprecipitation with anti-Flag antibody followed by Western blot analysis with anti-HA ...vector control and HA-Ub ...
9
The transcription factor CREM drives an inflammatory phenotype of T cells in oligoarticular juvenile idiopathic arthritis
... PBMCs from healthy donors were incubated with 10% allogenic SF or serum from allogenic healthy controls (HC) in RPMI (Gibco, Germany) with 10% FCS (Bio- chrom, Germany). When indicated, cells were stimulated with ...
9
CDK2 Regulates HIV 1 Transcription by Phosphorylation of CDK9 on Serine 90
... We recently showed that iron chelators inhibit CDK2 ac- tivity and HIV-1 transcription [28,29]. We also observed that iron chelators inhibit CDK9 activity [28]. The inhib- ition of CDK9 by iron chelators led to the ...
16
KDM1A promotes tumor cell invasion by silencing TIMP3 in non-small cell lung cancer cells
... JNK is an important kinase that is involved in regulating cell polarity and migration. We next sought to determine whether KDM1A-mediated cell invasion and migration involves JNK activation. First, we overexpressed KDM1A ...
16
Tombusvirus P19 RNA silencing suppressor (RSS) activity in mammalian cells correlates with charged amino acids that contribute to direct RNA-binding
... (nt) siRNA duplex in a positively charged surface cleft, but that this binding is progressively weaker for a siRNA of 23 – 26 nt in size and become even weaker for a 19 nt siRNA ...the siRNA ...
10