coomassie blue
Adapted mode of the mechanism of the photooxidation of coomassie blue with hydrogen peroxide by ph variations in aqueous medium
... , Ti 2+ , Zn 2+ as the catalyst have the problem of the suspension of these catalyst in water as stable hydrocolloid which lacks its separation from water and al., 2010). Photo assisted methods use photocatalyst which ...
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Epstein-Barr virus latent membrane protein 2 associates with and is a substrate for mitogen-activated protein kinase.
... Left In vitro kinase assay with affinity-precipitated proteins from BJAB cell extracts uninduced U or induced I for 15 min with anti-IgM Fab92; middle Coomassie blue staining of GST and [r] ...
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Comparison of cell wall proteins in putative Candida albicans & Candida dubliniensis by using modified staining method & SDSPAGE
... CA), Coomassie Brilliant Blue staining according to Lee et ...and Coomassie Blue staining accord- ing to Joseba and Chaffin observations ...
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IMPACT OF SUB INHIBITORY CONCENTRATIONS OF AMOXICILLIN ON HELICOBACTER PYLORI VIRULENCE FACTORS
... Preliminary evaluation of the extracts was performed using SDS–PAGE with a 12.5% acrylamide gel. Ten micrograms (10 μg) of each extract were applied to the gel, and the proteins were separated at a constant 200 V. ...
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A simple in vitro method to measure autophosphorylation of protein kinases
... mL Blue Silver Coomassie stain (0.12% Coomassie Blue G-250, 10% am- monium sulfate, 10% phosphoric acid, and 20% methanol) for at least one hour, and destained for one hour in destaining ...
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Quantitation of RNA tumor viruses by spectroscopy of density gradient gels.
... Using 0.8-ml gradients gels 4 by 45 mm we can detect as low as 2 x 107 viral particles with Coomassie blue staining and 6 x 106 viral particles with a more sensitive staining procedure u[r] ...
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Viral Macro Domains Reverse Protein ADP-Ribosylation
... The HEV helicase domain stimulates de-PARylation but not de-MARylation. We performed time courses of de-MARylation and de-PARylation to further understand the role of the HEV helicase domain in these reactions. The macro ...
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Identification of a Novel 74 Kilodalton Immunodominant Antigen of Pythium insidiosum Recognized by Sera from Human Patients with Pythiosis
... Identification of immunodominant antigens of P. insidio- sum. We studied the protein profile and immunogenicity of SABHs as cytoplasmic antigens and CFAs as secretory/culture supernatant antigens of P. insidiosum ...
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Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system.
... Coomassie blue-stained SDS-10% polyacrylamide gel A and immunoblot B analysis of total lysates of Sf9 cells infected with three different recombinant AcNPVs expressing the structural pro[r] ...
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Synthesis of viruslike particles by expression of the putative capsid protein of Leishmania RNA virus in a recombinant baculovirus expression system.
... Western blot analysis B and Coomassie blue staining A of proteins from lysates of 3 x 106 SF9 cells in 100-mm tissue culture dishes mock-infected lanes 2 or infected with wild-type AcMNP[r] ...
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Lactococcus lactis provides an efficient platform for production of disulfide-rich recombinant proteins from Plasmodium falciparum
... lactis raw culture supernatant expressing R0-MSPDBL2 fusion protein; Left panel: Coomassie blue stained SDS-PAGE analysis of R0-MSPDBL2 containing culture supernatant before (lane 1) [r] ...
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Alteration of renal respiratory Complex-III during experimental type-1 diabetes
... with Coomassie blue staining solu- tion (0.1% bromophenol blue R-250 in 40% methanol and 10% acetic acid) for 30 minutes and then destained overnight in ...
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Proteins, Detection of pdf
... equivalent of colloidal Coomassie Blue staining. The poor performance of these dyes resulted in a recent investigation of luminescent metal chelate stains. Such stains utilizing metal chelates complexed to ...
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Combination of Paclitaxel and MG1 oncolytic virus as a successful strategy for breast cancer treatment
... Coomassie Blue staining of the various breast cancer cell lines 48 h post infection with MG1-GFP with or without pre-treatment with recombinant murine IFNβ.. PAC and MG1 synergistically [r] ...
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Proteomic identification of heat shock protein 90 as a candidate target for p53 mutation reactivation by PRIMA 1 in breast cancer cells
... a Coomassie blue-stained gel of proteins co- immunoprecipitated with DO-1 antibody from MDA-MB-231 cells (mut p53) after treatment with 100 µM PRIMA-1 for 4 ...
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Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4.
... frugiperda SF9 cells, demonstrated by Coomassie blue staining A and Western blot analysis B of proteins from lysates of 105 SF9 cells mock infected lanes 2, infected with wild-type AcMNP[r] ...
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Electrophoretic characterization of soluble protein extracts of Legionella pneumophila and other members of the family Legionellaceae
... Figures 1 and 2 show the patterns obtained with Coomassie blue staining of soluble protein extracts from isolates representing each of the six serogroups of L.. The patterns are visually[r] ...
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El-Aouni, Chiraz (2006): In Vivo modulation of integrin linked kinase using transgenic mice. Dissertation, LMU München: Fakultät für Biologie
... SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ... Western Blot ... Coomassie blue staining of protein gels ... Drying of SDS-PAGE gels.... Histological techniques ... Frozen sections[r] ...
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Molecular properties of the Ro/SSA antigen and enzyme linked immunosorbent assay for quantitation of antibody
... and Coomassie Blue, Western blotting, and RNAase treatment leads to a hypothesis for the structure of the particle in which an antigenic 60,000 protein is bound to 24,000-27,000 RNA molecules which are not ...
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Association of the Transformation-Specific Protein pp60src with the Membrane of an Avian Sarcoma Virus
... D Fifty microliters of each indicated fraction of the membrane gradient was analyzed on a sodium dodecyl sulfate-polyacrylamide gel which was stained for proteins by Coomassie brilliant [r] ...
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