Corneal epithelial stem cells
Efficacy of cultivated corneal epithelial stem cells for ocular surface reconstruction
... the corneal surface and symblepharon were completely ...limbal epithelial sheet with an AM carrier was then removed from the culture disc, transferred onto the corneal surface, and sutured with 10-0 ...
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Anatomical location and culture of equine corneal epithelial stem cells
... Eyeballs were collected from adult thoroughbred horses that were being killed in a slaughterhouse and were imme- diately transported to our laboratory in phosphate- buffered saline. After confirming that the tissues were ...
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Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells
... iPS cells with defined factors, cells can be directly reprogrammed into another cell type without dedifferentiation to a pluripotent state by introducing tissue specific transcription factors ...the ...
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Xenofree generation of limbal stem cells for ocular surface advanced cell therapy
... The maintenance of the size and the morphology of LSC have deep implications in their characterization [1, 2, 32]. LSC cultured with XSHEM media were smaller, with higher nucleus-to-cytoplasm ratio, and the cultures ...
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Rat limbal niche cells can induce transdifferentiation of oral mucosal epithelial cells into corneal epithelial-like cells in vitro
... embryonic stem cell medium (MESCM), which consisted of DMEM/F12 supplemented with 10% knockout serum, 5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL sodium selenite, 4 ng/mL basic fibroblast growth factor, 10 ng/mL ...
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Limbal stem cell transplantation: current perspectives
... limbal epithelial stem cells and their key role in maintaining corneal ...of cells from the “healthy donor eye” to restore the ocular surface of the “diseased eye” with acceptable ...
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Characterisation and evaluation of the impact of microfabricated pockets on the performance of limbal epithelial stem cells in biodegradable PLGA membranes for corneal regeneration
... limbal stem cell niches in the eye and they can be custom-designed as previously described by our group using a combination of ...of epithelial cell trans- fer (f ) and explant outgrowth ( j) from an ...
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Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation
... and epithelial transdifferentiation of the three subpopulations with subsequent analysis at the gene and protein ...of corneal epithelium, after differentiation medium was ...contained cells ...
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Cellular therapy of corneal epithelial defect by adipose mesenchymal stem cell-derived epithelial progenitors
... closed corneal surface reduced the exposure of MET-Epi cells to air-liquid interphase that could have hindered the epithelial cell polarization and differenti- ation ...MET-Epi cells derived ...
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A conditional Pax6 depletion study with no morphological effect on the adult mouse corneal epithelium
... The corneal epithelium is maintained by limbal epithelial stem cells (LESCs), in the ring-shaped limbus, which is a transition zone between the corneal epithelium and the ...amplifying ...
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Mesenchymal stem cells for treating ocular surface diseases
... embryonic stem cell marker [42], have higher potential to differentiate into corneal epithelial cells than SSEA4 negative ...of stem cell markers, such as Sox2, Oct4, Nanog, Rex1, ABCG2 ...
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siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy
... expression. Cells were treated with NSC4 or K12-Leu132Pro-9 siRNA for a number of time ...K12-Leu132Pro-9–treated cells, where in cells treated with NSC4 K12 expression was unaffected ...MECD ...
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The characterisation of endometrial epithelial stem/progenitor cells
... It can be seen from the gel above that TERT 1 gave rise to multiple product bands within endometrial cancer 14 tissue. TERT 1 and TERT 2 both failed to detect expression within the Hues7 ESCs. TERT3 showed a consistent ...
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CD38 and CD157 Ectoenzymes Mark Cell Subsets in the Human Corneal Limbus
... of cells from the corneal limbus ...in corneal regeneration is the lack of a dependable surface marker of the limbal stem cell population ...human corneal epithelial ...
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A clinical study on the outcome of tenoplasty in ocular chemical injuries.
... limbal stem-cell function is ...of epithelial migration and adhesion and it also releases growth factors that facilitate proliferation of limbal stem cells and transient amplifying ...
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Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
... and also appear repelled by the scleral surface. Secondly, there may also be residual structural signals for cell arrangement left on the decellularized tissue surface which further aids the seemingly pre-programmed ...
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Comparison of single-step reverse transepithelial all-surface laser ablation (ASLA) to alcohol-assisted photorefractive keratectomy
... Sixty eyes of 30 myopic patients (19 male and 11 female) undergoing excimer photorefractive keratectomy were included. The average age was 29 years (SD: 9; range: 17–46). Preoperative mean UDVA was 0.01 Snellen fraction ...
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Epithelial Sodium Channels in Pulmonary Epithelial Progenitor and Stem Cells
... club cells and ATII ...H441 cells originally derived from human club ...native stem cells to club cells or alveolar cells [22, ...H441 cells [25-27], and respiratory ...
5
Primary graft failure associated with epithelial downgrowth: a case report
... Epithelial downgrowth is an unusual complication after penetrating keratoplasty [1-3,6-17]. (Table 1) The inci- dence in patients undergoing aphakic penetrating kerato- plasty has been estimated at 0.25% [3], and ...
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Cancer stem cells and oral cancer: insights into molecular mechanisms and therapeutic approaches
... the cells at the expense of ATP hydrolysis, and thus led to the decreased intra-cellular medicine con- ...normal cells. Overexpressing ABC transporters in cancer cells enhanced their chemo-radi- ...
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