cotyledon explants
Effect of Supplementing Mixture of Benzene Amino purine (BAP) and Kinetin (KN) Along with Auxin 2,4 D On Growth Of Callus In Murashige And Skoog Medium Derived From Embryonic Cotyledon Explants of Withania Somnifera L
... Cotyledon explants (size 1.5cm) were inoculated in full strength MS medium supplemented with 0.8% agar-agar and same concentration 2mg/l of 2, 4-D with 0.5mg/l to 1.5mg/l BAP, same concentration 2mg/l of 2, ...
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Establishment Of An Efficient In Vitro Regeneration System Of Ridge Gourd (Luffa Acutangula L. Roxb) From Immature Embryo And Cotyledon Explants
... Abstract: Ridge gourd (Luffa acutangula L. Roxb) is a popular and nutritious vegetable in Bangladesh. The present investigation was undertaken to establish an efficient in vitro regeneration system of ridge gourd from ...
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Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens
... Abstract: The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium- mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and ...
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Impact of culture media and growth hormones on callus induction and root organogenesis in cotyledon segment of Anacardium occidentale L.
... in cotyledon segments were not seems to be reported ...of cotyledon segments after 2 weeks of culture; furthermore, entire surface of explants were covered with callus within 4 weeks of culture ...
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Characterization of antihyperglycemic and antiretroviral components of Momordicacharantia(Bitter melon)
... internodal explants derived from 30 day old in vivo plants of bitter melon was evaluated on MS medium supplemented with individual treatment of different auxins (NAA and 2,4-D) or their combination, either with ...
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SUMMARY The shoot regeneration response from different explants, viz . cotyledon, cotyledonary node and petiolule, of the most popular Indian cultivar, JL-24 of peanut ( Arachis hypogaea L.), was evaluated by culturing on Murashige and Skoog medium containing B
... the explants were cut vertically into two halves along the embryo ...the cotyledon was damaged vertically to inhibit the growth of any primary ...node explants so prepared was cultured on MS medium ...
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Effect of gamma radiations on in vitro regeneration in Brassica carinata A. Braun Vedna Kumari , H.K. Chaudhary , R. Prasad , A. Kumar , S. Jambhulkar and S. Sharma
... of cotyledon explants within 3 ...Hypocotylar explants produced more shoots than cotyledon explants but, fewer roots in different cultivars of Brassica oleracea ...
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The effect of different concentrations of TDZ and BA on in vitro regeneration of Iranian cannabis (Cannabis sativa) using cotyledon and epicotyl explants
... epicotyl explants and cotyledon explants showed no response of shooting (Figure ...both cotyledon and epicotyl explants under treatments with TDZ and IBA (Figure ...epicotyl ...
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A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)
... old cotyledon explants on a variety of solid media, and different hor- mone combinations have been evaluated over the years for the best regeneration results [12–14, ...
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Effect Of Hormone Concentrations And Age Of Explant On Multiple Shoot Regeneration From Cotyledon Of Cucumber
... light. Cotyledon explants excised from 5-day- old in vitro raised seedlings of cucumber (cucumissativus ...When cotyledon explants were cultured on MS medium containing ...of explants ...
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Influence of cotyledon, hypocotyl extracts and authentic andrographolide on selective Agrobacterium rhizogenes strains growth: A deterrent to hairy root induction in Andrographis paniculata (Burm.f.) Wall. ex Nees
... Andrographis paniculata (Burm.f.) Wall. ex Nees is an important source of diterpene lactone andrographolide with a wide range of medicinal properties. The present study was initiated to induce hairy roots through ...
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Use of proximal hypocotyl segment for high-through put transgenic development of tomato
... of explants in highest shoot regeneration, this is probably due to the variation in the ...that cotyledon explants are superior (Ajenifujah-Soleboet ...to cotyledon in terms ofshoot ...to ...
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Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl)
... The result reviled in Table 1 indicated that the highest percentage of callus induction (89%) was observed on MS media supplemented by 8.00 mg l -1 2, 4-D. While poor (11%) or no response was observed on media ...
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The gene ENHANCER OF PINOID controls cotyledon development in the Arabidopsis embryo
... the cotyledon boundary regions (Long et ...in cotyledon primordia at the heart stage and in the vascular precursor cells as of the torpedo stage (Elliott et ...
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In Vitro Propagation and Development of Salt Tolerant Lines of Edible and Medicinal Varieties of Coleus sp.
... Callusing: There was no callus induction of Coleus parviflorus in plain MS media. However, nodal explants of Coleus forskohlii exhibited some amount of callus growth in MS media. From this it may be stated that ...
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The role of the cotyledon on the flowering behaviour of Pisum Sativum : a physiological study
... It can be seen that vernalization and cotyledon excision led to advancement of NF in both photoperiods, and that the two treatments supplemented one another in effect, the maximum advanc[r] ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... The 3 ́ RACE System for Rapid Amplification of cDNA Ends (Invitrogen) was used. First strand cDNA synthesis was catalyzed by SuperScript II reverse transcriptase (Invitrogen) using 5 µg of total RNA from sunflower ...
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INHERITANCE OF COTYLEDON, SEED-COAT, HILUM AND PUBESCENCE COLORS IN SOY-BEANS
... The two varieties which furnished the foregoing data on cotyledon and seed-coat colors also diflered in color of hilum or (‘eye.” I n seeds of variety 8, the original hilum co[r] ...
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Epiblast specific Snai1 deletion results in embryonic lethality due to multiple vascular defects
... allantois explants The Snai1 and Snai2 genes have a demonstrated role in the protection of cells from apoptotic cell death ...allantois explants by TUNEL assay. Snai1-cko explants exhibited an ...
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Oryzalin and Gamma Radiation Induced Polyploidization in Garden Balsam Plants (Impatiens balsamina L.) In Vitro
... and planted in the field to collect seeds for in vitro study to induce a new type of mutant plant. In vitro methods was conducted to achieved rapid micropropagation of Impatiens balsamina L. mutant plants. Growth ...
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