counting kit-8
Anti adipogenic effects of the traditional herbal formula Dohongsamul tang in 3T3 L1 adipocytes
... cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, ...(TG) kit was obtained from Bioassay Systems (CA, USA) and leptin ELISA kit was purchased from ...
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Overexpression of TGFβ1 in murine mesenchymal stem cells improves lung inflammation by impacting the Th17/Treg balance in LPS-induced ARDS mice
... Cell counting kit-8; CDS: Coding sequence; DMEM/ F12: Dulbecco ’ s modified Eagle ’ s media/nutrient mixture F-12; EDTA: Trypsin- ethylenediaminetetraacetic acid; eGFP: Enhanced green fluorescent ...
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MicroRNA-384 inhibits proliferation migration and invasion of glioma by targeting at CDC42
... Notes: (A) The expression of mir-384 in normal human astrocytes (nhas), U251 and U87 transfected with mir-nc, mir-384 mimics, inhibitor-nc or mir-384 inhibitor were detected by real-time polymerase chain reaction ...
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OX40 signaling is involved in the autoactivation of CD4+CD28− T cells and contributes to the pathogenesis of autoimmune arthritis
... Cell Counting Kit-8; CIA: Collagen-induced arthritis; C-CIA: Chronic collagen-induced arthritis; CII: Collagen type II; Cy7: Cyanine 7; DAS28: 28-joint Disease Activity Score; Dex: Dexamethasone; ...
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<p>Knockdown of lncRNA TDRG1 Inhibits Tumorigenesis in Endometrial Carcinoma Through the PI3K/AKT/mTOR Pathway</p>
... Human endometrial epithelial cell (hEEC) was purchased from the American Type Culture Collection (ATCC, Rockefeller, MD, USA). EC cell lines (HEC-1A and Ishikawa) were obtained from the Cell Bank of Chinese Academy of ...
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Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
... cell counting kit-8, Annexin V-FITC/PI Annexin V-fluorescein isothiocyanate/propidium iodide, qRT-PCR quantitative real-time PCR, ELISA enzyme-linked immune sorbent ...
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Combination of gambogic acid with cisplatin enhances the antitumor effects on cisplatin-resistant lung cancer cells by downregulating MRP2 and LRP expression
... GA (.98% purity, Sigma-Aldrich, St Louis, MO, USA) was soluble in dimethyl sulfoxide and conserved at - 20 ° C. The preservation solution was diluted to different concentra- tions in use, in which the dimethyl sulfoxide ...
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Anti-EGF Receptor Aptamer-Guided Co-Delivery of Anti-Cancer siRNAs and Quantum Dots for Theranostics of Triple-Negative Breast Cancer
... Cell growth inhibition by transfection with anti-cancer therapeutic siRNAs treated with QLs, aptamo-QLs, and immuno-QLs was measured using the Cell Counting Kit-8 (CCK-8, Dojindo Labo[r] ...
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Polyethylene glycol conjugated HER2 targeted peptides as a nuclear imaging probe for HER2 overexpressed gastric cancer detection in vivo
... AGC : adenocarcinoma of gastric cardia; AHNP: anti‑HER2/neu peptide; CCT‑8: cell counting kit‑8; CT: computed tomography; Cy5.5: cyanine5.5 NHS ester; DTPA: diethylenetriaminopentaacetic acid; ...
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<p><em>EPS8</em> is a Potential Oncogene in Glioblastoma</p>
... Abbreviations: EPS8, Epidermal growth factor receptor kinase substrate 8; GBM, glioblastoma; CCK-8, Cell Counting Kit-8; NC, untreated U87MG cells.. Yang et al Dove press.[r] ...
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Small cell lung cancer growth is inhibited by miR 342 through its effect of the target gene IA 2
... cell counting kit-8; DAPI: 4′,6-diamidino-2-phenylindole, dihydrochloride; DCV: dene-core vesicle; ELISA: enzyme-linked immunosorbent assay; FSH: follicular stimulating hormone; GAPDH: ...
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Protoporphyrin IX photobleaching of subcellular distributed sites of leukemic HL60 cells based on ALA PDT in vitro
... Cell Counting Kit-8 (CCK-8) as- say, and PpIX Photobleaching of subcellular distri- buted sites of HL60 cells in vitro were investigated by fluorescence spectra acquired during ...
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Original Article Effects of Btbd7 knockdown on the proliferation of human dental pulp cells and expression of Dspp
... cell counting Kit-8 assay, and expression of dentin sialophosphoprotein (Dspp) was assessed using real-time quantitative reverse transcription- PCR and Western ...
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Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function
... cell counting kit-8 analysis were conducted to assess the effects of UCB-Exos on migration and proliferation of human skin fibroblasts and endothelial ...
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Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats
... Cell counting kit-8; CTX: Cyclophosphamide; DAPI: 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; EDTA: Ethylenediaminetetraacetic acid; ELISA: Enzyme-linked immunosorbent assay; FDA: Food ...
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Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
... cell counting kit-8, plate colony formation assay, transwell migra- tion and invasion assays, and flow cytometry in vitro and by murine xenograft tumor growth in ...
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Metformin inhibits 17β-estradiol-induced epithelial-to- mesenchymal transition via βKlotho-related ERK1/2 signaling and AMPKα signaling in endometrial adenocarcinoma cells
... Metformin inhibits the 17β-estradiol-induced proliferation, migration, and invasion of endometrial adenocarcinoma cells The Cell Counting Kit-8 CCK-8 assays were performed to determine t[r] ...
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Anti inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo san in RAW 264 7 macrophages
... cell counting kit-8; cDNA, complementary DNA; COX, cyclooxygenase; ECL, enhanced chemiluminescence; ERK, extracellular signal-regulated kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GMSYS, ...
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Exosomes secreted by urine-derived stem cells improve stress urinary incontinence by promoting repair of pubococcygeus muscle injury in rats
... (8 weeks of age) were anesthetized with ketamine (50 mg/kg) intraperitoneally, and the forelimbs were fixed and dragged on the cage with a supine position. A cath- eter was inserted 2~3 cm into the rat’s vagina ...
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Original Article Silencing of CTHRC1 inhibits proliferation and metastasis of endometriotic stromal cells
... The relationship between CTHRC1 and tumor- regulated genes were explored by western blot assays. Transfected cells were harvest, washed twice with PBS, lysed in ice-cold radio in ice- cold radio immunoprecipitation assay ...
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