cowpea mosaic virus
Coalescence of the Sites of Cowpea Mosaic Virus RNA Replication into a Cytopathic Structure
... Cowpea mosaic virus (CPMV) replication induces an extensive proliferation of endoplasmic reticulum (ER) membranes, leading to the formation of small membranous vesicles where viral RNA replication ...
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Expression of Bottom Component RNA of Cowpea Mosaic Virus in Cowpea Protoplasts
... Comparison of the peptide patterns of the virusinduced proteins with those of the cowpea mosaic virus RNA-coded polypeptides produced in rabbit reticulocyte lysate showed that the 170- a[r] ...
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Interaction between a 54-Kilodalton Mammalian Cell Surface Protein and Cowpea Mosaic Virus
... Cowpea mosaic virus (CPMV), a plant virus that is a member of the picornavirus superfamily, is increasingly being used for nanotechnology applications, including material science, vascular ...
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The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
... Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as ...
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Evidence That the 32,000-Dalton Protein Encoded by Bottom-Component RNA of Cowpea Mosaic Virus is a Proteolytic Processing Enzyme
... The protease responsible for the cleavage of the M-RNA-encoded primary translation products is obviously coded for by the BRNA, since both in vitro translation products obtained from thi[r] ...
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The cowpea mosaic virus M RNA-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts.
... Employing a transient expression system based on the 35S promoter of cauliflower mosaic virus 11, we now show that synthesis of the 48K protein in the absence of the viral replication pr[r] ...
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Encapsidation of Viral RNA in Picornavirales: Studies on Cowpea Mosaic Virus Demonstrate Dependence on Viral Replication
... in virus-infected Nicotiana benthamiana (55), along with the P19 suppressor of gene silencing, which is always expressed from pEAQ-RNA-2 and therefore behaves as the genetic background in this ...
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Cowpea Mosaic Virus 32- and 60-Kilodalton Replication Proteins Target and Change the Morphology of Endoplasmic Reticulum Membranes
... Construction of the transient expression vectors. Plasmid pMON999 is a plant expression vector that contains the enhanced cauliflower mosaic virus (CaMV) 35S promoter, followed by a multiple cloning site ...
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Antibodies Against the Genome-Linked Protein VPg of Cowpea Mosaic Virus Recognize a 60,000-Dalton Precursor Polypeptide
... A protein blot of the membrane fraction of CPMV-infected cowpea leaves was prepared as described above and incubated with 30 ,ul of anti-VPg serum in 25 ml of RIA buffer which had not be[r] ...
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Orientation of the Cleavage Map of the 200-Kilodalton Polypeptide Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus
... Comparison of the proteolytic peptide patterns of 170K polypeptides synthesized in vitro and pulse-labeled at either the NH2-terminal or the COOH-terminal end with the patterns of the 17[r] ...
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Isolation and characterisation of mutants of cowpea mosaic virus
... However, it is a striking fact that the M RNAs of all como viruses studied so far cowpea severe mosaic virus, bean pod mottle virus, red clover mottle virus and squash mosaic virus, all [r] ...
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In Vitro Replication of Cowpea Mosaic Virus RNA III. Template Recognition by Cowpea Mosaic Virus RNA Replicase
... The RNAs from brome mosaic virus, tobacco streak virus, TYMV, and Semliki Forest virus, added up to a 50-fold molar excess of [3P]CPMV RNA, completely failed to compete with CPMV RNA in [r] ...
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Characterization of the 3′ Termini of the RNAs of Cowpea Mosaic Virus
... After fractionation of the samples on fresh columns of oligodT-cellulose, a portion of the material that bound [i.e., the polyA] was saved for electrophoresis on gels, and the material t[r] ...
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In Vitro Replication of Cowpea Mosaic Virus RNA I. Isolation and Properties of the Membrane-Bound Replicase
... DISCUSSION Our results show that CPMV-infected cowpea leaves contain an RNA-dependent RNA polymerase activity which appears to be closely bound with cytoplasmic membranes, and which is a[r] ...
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Determination of the Proteolytic Processing Sites in the Polyprotein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus
... Sequence analysis of the 32K protein, which is derived from the amino-terminal end of the 200K polyprotein, indicated that the AUG codon at nucleotide position 207 of the RNA sequence is[r] ...
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Distribution and Detection of Cowpea Viruses Infecting Cowpea in Uganda
... plant virus diseases have been recognized and documented, although it has only recently be- come possible to identify and study the causal ...for cowpea are caused by viruses and represent significant ...
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Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses.
... From the comparison of the protease amino acid sequences of animal picornaviruses, namely EMC virus 26 and foot-and-mouth disease virus 5, and cowpea mosaic virus 20, a plant comovirus, [r] ...
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Evaluation of Cowpea Resistance to Cowpea Aphid Borne Mosaic Virus in Burkina Faso
... plusieurs virus du niébé dont le Cowpea Aphid-Borne Mosaic Virus (CABMV), le Blackeye Cowpea Mosaic Virus (BICM), le Cowpea Yellow Mottle Virus (CYMV) [5], ...
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TRANSMISSION OF CUCUMBER MOSAIC VIRUS (CMV) INFECTING BANANA BY APHID AND MECHANICAL METHODS
... Even though CMV infecting other than banana can be transferred to many hosts for the purpose of propagation and purification, it is always difficult to transmit the CMV banana isolate to propagation hosts either ...
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EFFECT OF INTERCROPPING ON THE DEVELOPMENT OF CASSAVA BACTERIAL BLIGHT
... cassava mosaic disease by intercropping, but intercropping cowpea with maize or cassava did not show a clear effect on cowpea bacterial blight, though an additional yield from the minor crop could ...
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