Cre-Loxp
Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
... the Cre-loxP system, which has been used previously to regulate gene expression in a variety of ...two loxP elements inserted between the pro- moter and the coding sequence to inhibit ...pressed ...
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Cre/loxP-mediated excision of a neomycin resistance expression unit from an integrated retroviral vector increases long terminal repeat-driven transcription in human hematopoietic cells.
... the cre recombi- nase gene driven by the retroviral promoter and the puromycin resistance gene, whose expression is directed by the internal simian virus 40 (SV40) immediate early promoter ...pBabePuro- Cre ...
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Viral Cre-LoxP tools aid genome engineering in mammalian cells
... two LoxP sites, the Cre recombinase recognizes the lox P sites and cuts ...the LoxP sites determine whether Cre recombination induces a deletion, inversion, or chromosomal transloca- tion [21, ...
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Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System
... expressing Cre with the HD vector and a helper virus bearing a packaging signal flanked by loxP ...sites. Cre-mediated packaging signal excision renders the helper virus genome unpackageable but ...
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A murine leukemia virus with Cre LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions
... The 9.6 kb bands from the Akv-Y subclones represent the BsrG1 fragment of the original provirus containing the 3' copy of the EYFP gene (Fig. 1b). After Cre-recombinase- mediated deletion in the Akv-2XY subclones, ...
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Transgene Coplacement and High Efficiency Site-Specific Recombination With the Cre/loxP System in Drosophila
... ORBAN, P. BAKER, 1995 A system for inser- tional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre-lox. GEWR, 1992 Position-indepen- dent germlin[r] ...
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Molecular dissection of integrin signalling proteins in the control of mammary epithelial development and differentiation
... the Cre-LoxP system, we provide evidence that ILK, but not FAK, has a key role in lactogenesis in vivo and in the differentiation of cultured luminal epithelial ...
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miR-15a/16 reduces retinal leukostasis through decreased pro-inflammatory signaling
... Single-cell suspensions were prepared from neuronal retinal tissue of the miR-15a/16 floxed, miR-15a/16 cre/ loxP mice by incubating with 0.05% trypsin for 30 min at 37 °C. The cell suspension was then ...
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Dnmt1 in Intestinal Development and Cancer
... DNA methyltransferase 1 (Dnmt1) maintains DNA methylation following DNA replication, but little is known about its requirement for organ development. Previous studies of mice with global deletion of Dnmt1 failed to ...
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Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart
... The phenotype of GLUT4-null mice, in which GLUT4 expression was ablated in all tissues in which it is nor- mally expressed (heart, skeletal muscle, and adipose tis- sue), provided additional evidence that changes in the ...
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Gene trapping identifies transiently induced survival genes during programmed cell death
... using Cre-specific ...the Cre/loxP approach to enrich for transiently expressed genes that are difficult to isolate by standard methodology and therefore underrepresented in the nucleotide ...
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Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro
... Using the Cre-loxP reaction, we prepared full- length recombinant adenoviral DNA in vitro by recombination between a shuttle plasmid and adenovirus genomic DNA that was previously trunca[r] ...
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Requirements for Catalysis in Cre Recombinase
... the Cre-loxP reaction that allows for a comparison with similar structures from the related ...in Cre evolved to have functional roles in site-specific recombination, that are unnecessary for ...
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Morphogenesis of the right ventricle requires myocardial expression of Gata4
... Conclusion. Using Cre/loxP technology we have selectively inac- tivated Gata4 within the myocardium at early and late stages of heart development. At both time points, we demonstrated that Gata4 was an ...
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A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme
... To prove that this new genetic tool enables genetic tracing of two distinct progenitor populations simultaneously in vivo, we used both Nigri-nox and Cre-loxP to reassess the cellular contribution to ...
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Delta-like 1 regulates Bergmann glial monolayer formation during cerebellar development
... the Cre-loxP system with hGFAP- Cre mice, whereby the human GFAP promoter directs the expression of Cre ...the Cre-mediated deletion of Dll1 in cultured astrocytes that were derived ...
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A novel in vitro model for the assessment of postnatal myonuclear accretion
... the staining-based method had sufficient power to detect relevant differences in postnatal myonuclear accretion. Furthermore, the staining-based method displayed a sig- nificant inter-rater correlation and a moderate to ...
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Construction and Use of a Recyclable Marker To Examine the Role of Major Facilitator Superfamily Protein Members in Candida glabrata Drug Resistance Phenotypes
... the Cre-loxP-based recombination, which has been used extensively in a wide variety of genetic systems to remove chromosome segments of ...of Cre, the methionine-regulatable promoter (MET3) is used ...
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Original Article Brahma compensates the role of brahma-related gene 1 in liver regeneration of albumin-Cre; brahma-related gene 1 loxP/loxP mice
... tered an intravenous injection of Ad-shRNA/ Brm or controls in the tail, and the hepatic his- tological or functional abnormalities of the mice were observed. Although liver regenera- tion was much slower in ...
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PubMedCentral-PMC4862663.pdf
... a Cre-expressing line widely-used for tissue-specific recombination within the developing ENS and other neural crest ...the Cre sequence is inserted into a Wnt1 minigene construct between the endogenous ...
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