Cre recombinase
Requirements for Catalysis in Cre Recombinase
... Cre recombinase, a member of the tyrosine recombinase (YR) family of site-specific recombinases catalyzes DNA rearrangements using phosphoryl transfer chemistry that is identical to that used by the ...
220
Inducible and coupled expression of the polyomavirus middle T antigen and Cre recombinase in transgenic mice: an in vivo model for synthetic viability in mammary tumour progression
... and Cre recombinase protein and at a higher inten- sity than carcinoma stages, suggesting a potential damp- ening of transgene expression once the tumour has progressed (Figure ...heterogeneous Cre ...
14
Gene recombination in postmitotic cells Targeted expression of Cre recombinase provokes cardiac restricted, site specific rearrangement in adult ventricular muscle in vivo
... using Cre recombinase can circumvent these pitfalls, in principle, enabling temporal and spatial control of gene re- ...of Cre-mediated recombination in postmitotic ...achieve Cre-mediated ...
12
A New System for Stringent, High-Titer Vesicular Stomatitis Virus G Protein-Pseudotyped Retrovirus Vector Induction by Introduction of Cre Recombinase into Stable Prepackaging Cell Lines
... Efficient and precise site-specific recombination in the PtG-S2 chromosome after the introduction of AxCANCre. Southern blot analysis of PtG-S2 (or PtG-L1) chromosomal DNA was performed to test whether the loxP-specific ...
7
Expression and activity of osteoblast targeted Cre recombinase transgenes in murine skeletal tissues
... directed Cre recombinase specifically in osteoblasts (Dacquin et ...sufficient Cre expression to induce gene rearrange- ment in ...However, Cre expression driven by an artificial OG2 promoter ...
9
Deficiency of 14 3 3ε and 14 3 3ζ by the Wnt1 promoter driven Cre recombinase results in pigmentation defects
... promoter-driven Cre recombinase although 14-3-3ζ was deleted in all ...promoter-driven Cre recom- binases is responsible for the smaller body size in addi- tion to the 14-3-3ζ ...cells, Cre ...
6
Riese, Florian (2009): Genetic Targeting of Cre Recombinase to the Murine ACTH Receptor Locus. Dissertation, LMU München: Medizinische Fakultät
... of cre recombinase (Xu et ...when cre was expressed at high levels (Loonstra et ...to cre expression in spermatids has been demonstrated for the same reason (Schmidt et ...the cre/loxP ...
131
Myocardial deletion of Smad4 using a novel α skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract
... The skilled technical assistance of Mrs. Batchelor is gratefully acknowledged. Dr Edna C. Hardeman and Prof. Pierre Chambon are thanked for, respec- tively, gifting the α-skeletal actin promoter and Cre ...
10
Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression.
... of Cre protein expressed from ...Ad-encoded Cre protein was first determined in a cell-free ...the Cre-specific efficiency of recombination to be approxi- mately 50%, as judged by the relative ...
7
Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens
... Bone marrow was aspirated from the femur of trans- genic and wild-type mice and grown overnight in RPMI media supplemented with 15% FBS, (Gibco BRL). Subse- quently, cells were harvested according to standard cyto- ...
17
Cellular Transformation of Mouse Embryo Fibroblasts in the Absence of Activator E2Fs
... Binding of p53 by TAg results in p53 inactivation and stabili- zation; thus, TAg-transformed cells exhibit high levels of p53 (11). Accordingly, we observed an increase in p53 levels in all (wt and DKO) control MEFs ...
10
Inhibitory and excitatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1
... injection. Cre recombinase cDNA with a methallothionein I polyadenylation signal in pBS185 (Stratagene) was excised by XhoI and HindIII and subcloned into pBluescript (Stratagene) to generate ...
10
Measles Virus Infection of SLAM (CD150) Knockin Mice Reproduces Tropism and Immunosuppression in Human Infection
... expressing Cre recombinase from the CAG pro- moter (provided by ...by Cre recombinase-medi- ated excision of the neomycin resistance ...and Cre recombinase-medi- ated excision of ...
10
Fgf8 expression defines a morphogenetic center required for olfactory neurogenesis and nasal cavity development in the mouse
... using Cre-LoxP tissue- specific gene disruption to determine the role of FGF8 signaling in OE development from ...which Cre recombinase expression is controlled by Foxg1 (forkhead box G1, also known ...
13
Models of breast cancer: quo vadis, animal modeling?
... the Cre recombinase from bacteriophage P1, which catalyzes the excision of an essential element of a target gene (for instance a coding exon) located between two loxP (locus of X-ing) recognition ...the ...
8
Direct leptin action on POMC neurons regulates glucose homeostasis and hepatic insulin sensitivity in mice
... Leptin action on its receptor (LEPR) stimulates energy expenditure and reduces food intake, thereby lowering body weight. One leptin-sensitive target cell mediating these effects on energy balance is the proopiomelano- ...
11
Microglia lacking a peroxisomal β-oxidation enzyme chronically alter their inflammatory profile without evoking neuronal and behavioral deficits
... Cx3cr1- Cre mice [20] with floxed Mfp2 mice ...the Cre-recombinase gene in the genome and haploinsufficiency of CX3CR1 did not negatively influence microglial behavior in the ...both Cre-po- ...
15
Pharmacological and chemogenetic orexin/hypocretin intervention ameliorates Hipp-dependent memory impairment in the A53T mice model of Parkinson’s disease
... The first goal of this study was to determine if the orexin system impairments occurs with the early hippo- campus (Hipp) dependent memory deficits in A53T mice. The second goal was to determine if orexin inter- vention ...
16
Frequent Coinfection of Cells Explains Functional In Vivo Complementation between Cytomegalovirus Variants in the Multiply Infected Host
... expressing Cre recombinase was combined for coinfection with a mutant carrying Cre-inducible green fluo- rescent protein gene, and attenuated mutants were combined for coinfection with wild-type ...
11
Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
... a Cre-encoding gene which is specifi- cally active only in eukaryotic ...the Cre-Lox site-specific recombination sys- ...pressing Cre recombinase (AxCANCre) ...for Cre-mediated ...
10