Cre recombinase (Cre)
Requirements for Catalysis in Cre Recombinase
... Cre recombinase, a member of the tyrosine recombinase (YR) family of site-specific recombinases catalyzes DNA rearrangements using phosphoryl transfer chemistry that is identical to that used by the ...
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Expression and activity of osteoblast targeted Cre recombinase transgenes in murine skeletal tissues
... directed Cre recombinase specifically in osteoblasts (Dacquin et ...sufficient Cre expression to induce gene rearrange- ment in ...However, Cre expression driven by an artificial OG2 promoter ...
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Deficiency of 14 3 3ε and 14 3 3ζ by the Wnt1 promoter driven Cre recombinase results in pigmentation defects
... We found that the 14-3-3ε/14-3-3ζ/Wnt1-Cre mice had white patches in their fur on the ventral region of their torso. In the Wnt1-Cre mice, Cre recombinase is expressed in neural crest cells ...
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A New System for Stringent, High-Titer Vesicular Stomatitis Virus G Protein-Pseudotyped Retrovirus Vector Induction by Introduction of Cre Recombinase into Stable Prepackaging Cell Lines
... Strategy for the generation and activation of the prepack- aging cell lines. For the generation of prepackaging cell lines, we made use of a human cell line, FLY, which expresses the gag and pol genes of MoMLV at a high ...
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Gene recombination in postmitotic cells Targeted expression of Cre recombinase provokes cardiac restricted, site specific rearrangement in adult ventricular muscle in vivo
... using Cre recombinase can circumvent these pitfalls, in principle, enabling temporal and spatial control of gene re- ...of Cre-mediated recombination in postmitotic ...achieve Cre-mediated ...
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Riese, Florian (2009): Genetic Targeting of Cre Recombinase to the Murine ACTH Receptor Locus. Dissertation, LMU München: Medizinische Fakultät
... of cre recombinase (Xu et ...when cre was expressed at high levels (Loonstra et ...to cre expression in spermatids has been demonstrated for the same reason (Schmidt et ...the cre/loxP ...
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Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression.
... of Cre protein expressed from ...Ad-encoded Cre protein was first determined in a cell-free ...the Cre-specific efficiency of recombination to be approxi- mately 50%, as judged by the relative ...
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Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens
... This work represents many years of effort and received input from many people. In particular, the authors would like to acknowledge Dr. Richard Maas as the original source for the P0-3.9GFPCre transgenic mice and Dr. ...
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Measles Virus Infection of SLAM (CD150) Knockin Mice Reproduces Tropism and Immunosuppression in Human Infection
... expressing Cre recombinase from the CAG pro- moter (provided by ...by Cre recombinase-medi- ated excision of the neomycin resistance ...and Cre recombinase-medi- ated excision of ...
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Models of breast cancer: quo vadis, animal modeling?
... the Cre recombinase from bacteriophage P1, which catalyzes the excision of an essential element of a target gene (for instance a coding exon) located between two loxP (locus of X-ing) recognition ...the ...
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Direct leptin action on POMC neurons regulates glucose homeostasis and hepatic insulin sensitivity in mice
... Leptin action on its receptor (LEPR) stimulates energy expenditure and reduces food intake, thereby lowering body weight. One leptin-sensitive target cell mediating these effects on energy balance is the proopiomelano- ...
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Microglia lacking a peroxisomal β-oxidation enzyme chronically alter their inflammatory profile without evoking neuronal and behavioral deficits
... Cx3cr1- Cre mice [20] with floxed Mfp2 mice ...the Cre-recombinase gene in the genome and haploinsufficiency of CX3CR1 did not negatively influence microglial behavior in the ...both Cre-po- ...
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Cellular Transformation of Mouse Embryo Fibroblasts in the Absence of Activator E2Fs
... FIG 1 SV40 TAg does not require activator E2Fs to induce proliferation in MEFs. (A) Western blot analysis showing SV40 TAg expression in wt and DKO backgrounds. (B) Growth curve comparison between wt and DKO MEFs in the ...
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Behavioral and transcriptome alterations in male and female mice with postnatal deletion of TrkB in dorsal striatal medium spiny neurons
... D9-Cre recombinase mouse [14] have been previously ...allele. Cre recombinase is detected using a second set of sequence-specific primers ...
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Fgf8 expression defines a morphogenetic center required for olfactory neurogenesis and nasal cavity development in the mouse
... using Cre-LoxP tissue- specific gene disruption to determine the role of FGF8 signaling in OE development from ...which Cre recombinase expression is controlled by Foxg1 (forkhead box G1, also known ...
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Pharmacological and chemogenetic orexin/hypocretin intervention ameliorates Hipp-dependent memory impairment in the A53T mice model of Parkinson’s disease
... The first goal of this study was to determine if the orexin system impairments occurs with the early hippo- campus (Hipp) dependent memory deficits in A53T mice. The second goal was to determine if orexin inter- vention ...
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Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes
... of Cre recombinase to excise the inserted dual-marker cassette once a recombinant animal is identified and ...after Cre-mediated recombination and removal of the cas- sette, a single loxP site remains ...
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Inhibitory and excitatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1
... express Cre recombinase from the Ptf1a locus or under the control of the Atoh1 promoter revealed that inhibitory (GABAergic and glycinergic) or excitatory (glutamatergic) neurons of both DCN and VCN are ...
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Frequent Coinfection of Cells Explains Functional In Vivo Complementation between Cytomegalovirus Variants in the Multiply Infected Host
... expressing Cre recombinase was combined for coinfection with a mutant carrying Cre-inducible green fluo- rescent protein gene, and attenuated mutants were combined for coinfection with wild-type ...
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Local production of angiotensin II in the subfornical organ causes elevated drinking
... the Cre-loxP recombinase system offers a unique tool to dissect neural pathways in the brain ...target Cre recombinase expression to specific cell types ...of Cre recombinase ...
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