CRISPR/Cas
A New Group of Phage Anti CRISPR Genes Inhibits the Type I E CRISPR Cas System of Pseudomonas aeruginosa
... I-E CRISPR-Cas system of strain SMC4386 was isolated, we were able to test di- rectly the type I-E anti-CRISPR activity of individual genes from phage anti-CRISPR regions by expressing them ...
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Development and Applications of CRISPR-Cas Based Precision Antimicrobials.
... of CRISPR-Cas systems in bacteria have significantly expanded molecular tools available for genome editing, but precision genetic applications, in ...repurposing CRISPR-Cas systems as ...
84
Diversity of CRISPR Cas immune systems and molecular machines
... bacteriophages, CRISPR interference is a potent barrier to natural DNA transformation that can be har- nessed to prevent the acquisition of virulence traits ...of CRISPR-Cas systems for the genesis ...
11
Ushering in CRISPR/Cas Mediated Genome Engineering for Crops
... The CRISPR toolbox is adapted from a prokaryotic RNA-mediated type II system that is involved in providing defence against invading ...into CRISPR array. Transcription of CRISPR arrays creates ...
8
Advances in Engineering the Fly Genome with the CRISPR-Cas System
... T HROUGH more than a century of study and extensive development of genetic tools, Drosophila melanogaster has become a premier system for understanding complex bi- ological processes at the molecular, cellular, and ...
18
Discovering, Characterizing, and Applying CRISPR-Cas Systems in Bacteria.
... associated Cas (CRISPR associated) proteins form widespread adaptive immune systems in prokaryotes that have been harnessed as ubiquitous tools in biotechnology and medicine 1–6 ...eukaryotes, ...
171
Meeting report: GARNet/OpenPlant CRISPR-Cas workshop
... Sainsbury Laboratory, Norwich (TSL), the primary organiser, who provided a historical perspective on GE technologies. The specifics of these technologies are dis- cussed in detail as part of this Plant Methods thematic ...
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Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems
... several CRISPR-Cas systems apparently remain functional despite having a highly degraded form of the large subunit (type U system) or lacking the large subunit altogether in some variants of Subtype I-C and ...
27
Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins
... Balance is a widespread natural principle in the evolu- tion of species [61–63]. The phage–bacterial arms race is a typical case to study this behavior. In order to sur- vive, bacteria exploit multiple types of ...
11
CRISPR-Cas Systems for Diagnosing Infectious Diseases
... on CRISPR-based technologies and their perspectives to become platforms for point-of-care nucleic acid detection methods and as deployable diagnostic platforms that could help to identify and curb outbreaks and ...
19
CRISPR-Cas Targeting and Escape in Lactobacillus gasseri.
... that CRISPR was involved in heritable cell immunity through inferences based on the amino acid composition and domain functions of the Cas proteins (Makarova, Grishin, Shabalina, Wolf, & Koonin, ...that ...
159
Disabling a Type I E CRISPR Cas Nuclease with a Bacteriophage Encoded Anti CRISPR Protein
... Transcriptional repression assay. Pairs of complementary oligonucleotides were designed to encode a CRISPR repeat-spacer-repeat unit in which the repeat sequence is the P. aeruginosa SMC4386 consensus repeat ...
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CRISPR-Cas Systems in Lactic Acid Bacteria.
... resistance. CRISPR-Cas systems consist of arrays of short DNA repeats interspaced by hypervariable sequences, flanked by cas genes, that provide adaptive immunity against invasive genetic elements ...
190
CRISPR-Cas Systems and Genetic Organization in Lactic Acid Bacteria.
... of CRISPR-Cas systems provide adaptive immunity against phages and plasmids in many bacteria and most ...into CRISPR loci, and immunity hinges on interfering CRISPR transcripts that guide ...
303
Just how Lamarckian is CRISPR-Cas immunity: the continuum of evolvability mechanisms
... of CRISPR spacers have identified a small fraction of sequences that were (nearly) identical to fragments of phage or plasmid genomes whereas the rest of the spacers showed no significant similarity to any ...
9
Escherichia Coli CRISPR/CAS System Evolved with Bacteriophage: A Case Report
... In this case study, we demonstrated the results of sequence analysis which can help understanding the presence of CRISPR-me- diated anti-phage ability in pathogenic and non-pathogenic strains of E. coli . We ...
7
Recent Advances in CRISPR/Cas Mediated Genome Editing and Applications
... sequence. CRISPR/Cas seems to be more promising, efficient and less time ...in CRISPR/Cas and its applications in crop improvement and ...
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Understanding and Exploiting the Properties of Type I CRISPR-Cas Systems
... Beyond the co-regulation of multiple genes, CRISPR-Cas systems are now being implemented for the design of genetic circuits. While the first examples were published in mammalian cells (Kiani et al., 2014; ...
167
ONLINE AND OFFLINE TOOLS: CRISPR/CAS OFF TARGET DETECTION
... rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 ...
7
Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana
... in CRISPR-Cas [15, 27, ...the CRISPR-Cas method and bypasses the need for co-transformation as it enables assembly of multiple expression units, such as Cas9 and sgRNAs, into a single vector ...
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