culture supernatant
Developing Rat Bone Marrow Derived Mast Cells by the Splenic Cells Culture Supernatant of Rat and Mouse
... yield. The floating cells, 18 and 19 days after the fourth culture gave rise to condensation with more homogeneous appearance. After 4 to 5 passages, on day 23, the cell morphology seems in mature format ...
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Effect of Physical and Chemical Changes on the Antimicrobial Activity of Culture Supernatant Fluid of Lactic Acid Bacteria
... of culture medium to about ...the culture supernatant fluid of Lactobacilli with different enzymes as well as neutralization using NaOH 1N and one hour incubation, the percentage of difference ...
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Effects of Lactobacillus casei Culture Supernatant on Differentiation of K562 Cell Line
... Methods: After culturing chronic myeloid leukemia cell line K562 in 96-well plates, effects of different concentrations of culture supernatant from Lactobacillus casei on differentiation of the cells were ...
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Antagonistic activities of some probiotic lactobacilli culture supernatant on Serratia marcescens swarming motility and antibiotic resistance
... test culture was added. Triplicate wells for each test culture, one well which nothing was added (no su- pernatant or media) just 100 µl of diluted test culture; one well, served as a negative ...
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Protein A Is Released into the Staphylococcus aureus Culture Supernatant with an Unprocessed Sorting Signal
... DNA encoding the sorting signal of SdrE was amplified by PCR using primers RESF.. and RESR using genomic DNA from strain Newman as template.[r] ...
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Comparison of MagPix Assays and Enzyme Linked Immunosorbent Assay for Detection of Hemorrhagic Fever Viruses
... microspheres from each set were added to every well of a Costar white polystyrene, 96-well plate. Next, 96 unique, normal human serum samples (Covance, Princeton, NJ) were diluted 1:5 in PBS-BT. Eight of these serum ...
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Hepatitis E Virus (HEV) Strains in Serum Samples Can Replicate Efficiently in Cultured Cells Despite the Coexistence of HEV Antibodies: Characterization of HEV Virions in Blood Circulation
... cell culture super- natant fluids sedimented heterogeneously and less rapidly than normal virus in rate-zonal sucrose gradients and also banded at a lower density in CsCl ...and culture supernatant ...
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Cytokine-Induced Killer Cells as an Adoptive Cellular Immunotherapy Strategy for Hepatocellular Carcinoma
... handled culture method for sufficient expansion of viable and cytotoxic cytokine-induced killer (CIK) cells against ...static culture to remove adherent ...the culture supernatant was ...
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Ghrelin Regulates Energy Homeostasis Through Discrete Central and Peripheral Signaling Mechanisms
... cells culture supernatant induced arginase-1 expression in bone marrow derived MDSC suggesting a difference in soluble factors released from activated T cells using different ...
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Characterization and antimicrobial activity of silver nanoparticles synthesized by rice straw utilizing bacterium (Lysinibacillus fusiformis)
... bacterial culture supernatants like Bacillus megaterium; Pseudomonas proteolytica; Pseudomonas meridian; Pseudomonas aeruginosa; Arthrobacter kerguelensis; Bacillus indicus: Sphingobacterium muzetti were proven to ...
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Mesenchymal stem cells promote pancreatic adenocarcinoma cells invasion by transforming growth factor-β1 induced epithelial-mesenchymal transition
... the culture supernatant of MSCs stimulated with the combination of TNF-α and ...the culture supernatant of MSCs (Figure 4A, ...the culture supernatant of MSCs pretreated with ...
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The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms
... Legionella pneumophila is the pathogen responsible for severe pneumonia known as Legionnaires’ disease. Legionella can live under varied stress conditions, especially in cold environments, and is common in many ...
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Transforming potential of a myc-containing variant of feline leukemia virus in vitro in early-passage feline cells.
... By comparison, the encapsidated myc-FeLV genome represented 14% of the total population in the culture supernatant of feline embryonic fibroblasts partially transformed by infection with[r] ...
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Purification, characterization and in vitro cytotoxicity of plantaricin 9496: a bacteriocin produced by lactobacillus plantarum mtcc 9496
... from culture supernatant. The cell culture after 16 hours of incubation was heated in boiling water bath for 20 minutes and was cooled ...the culture was adjusted to ...of supernatant ...
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Development of a Simple, Peripheral-Blood-Based Lateral-Flow Dipstick Assay for Accurate Detection of Patients with Enteric Fever
... Detection of S. Typhi LPS-specific antibodies in specimens by ELISA. We compared strip results to those obtained using an anti-S. Ty- phi LPS ELISA format, as previously described (10). Briefly, we coated microtiter ...
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Inactivation of Streptococcus pyogenes extracellular cysteine protease significantly decreases mouse lethality of serotype M3 and M49 strains
... the culture supernatants were precip- itated by treatment with ethanol, separated by elec- trophoresis, and transferred to a nitrocellulose ...concentrated culture supernatant proteins of M3 ...
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In Vitro Release by Aspergillus fumigatus of Galactofuranose Antigens, 1,3 β d Glucan, and DNA, Surrogate Markers Used for Diagnosis of Invasive Aspergillosis
... the culture filtrates is probably even higher because the assay uses synthetic PNP-galf as a ...the culture supernatant might also decrease the measured en- zyme ...
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Original Article Role of TGF-β1/p38 MAPK pathway in hepatitis B virus-induced tubular epithelial-myofibroblast transdifferentiation
... thelial cell polarity. Zhong et al. [18] show that, in normal human kidney tissues, E-cadherin was abundantly expressed. On the other hand, in patients with type 2 diabetes, the expression of E-cadherin was dramatically ...
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Spontaneous Mesenchymal to Epithelial Like Tissue Transition (MET) in a Long Term Human Skin Culture
... Mesenchymal- epithelial transition is a biological process which plays some important roles in health and diseases. For instances, it is involved in decidualization which in turn is considered as a vital phenomenon in ...
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New, practical approach to detecting antibody to pertussis toxin for public health and clinical laboratories
... pertussis in a simple, defined medium to obtain a PT-rich supernatant with little contamination of cellular antigens; ià a simple, one-step concentration of PT in the culture supernatant[r] ...
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