Cyclic nucleotide phosphodiesterase activity
Inhibitors of cyclic nucleotide phosphodiesterase isozymes type III and type IV suppress mitogenesis of rat mesangial cells
... cAMP- phosphodiesterase (PDE) isozyme PDE-III, inhibited mitogenesis to similar extent as forskolin and DBcAMP and activated in situ PKA, but without detectable increase in cAMP ...MAPK activity (+300%); ...
11
Expression and characterisation of a novel cyclic nucleotide phosphodiesterase Type lA from dog heart
... calmodulin activity despite successive chromatographic purification procedures carried out (Sharma and Wang, ...CaM-PDE activity increased as the Ca^^ concentration was increased, and that there was no ...
350
Expression of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and its roles in activated microglia in vivo and in vitro
... 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is a protein that hydrolyzes cyclic nucleotides to mono- phosphates (2′ ...enzyme activity were found in membranes of cells in the ...
17
Expression, purification, and initial characterization of different domains of recombinant mouse 2',3' cyclic nucleotide 3' phosphodiesterase, an enigmatic enzyme from the myelin sheath
... enzymatic activity of CNPase, the hydrolysis of 2 ’ ,3 ’ -cyclic nucleotides to 2 ’ -nucleotides, was the basis of the discovery of the enzyme from brain tissue in the 1960s ...The activity is ...
7
Regulation of endothelial cell cyclic nucleotide metabolism by prostacyclin
... of cyclic AMP phosphodiesterase activity because significant PGI2- mediated increases in cyclic AMP could not be shown in the presence of ...
8
Calmodulin stimulated cyclic nucleotide phosphodiesterase (PDE1C) is induced in human arterial smooth muscle cells of the synthetic, proliferative phenotype
... A PDE1B isoform was detected in the smooth muscle iso- lated from the medial layer of human aorta but is absent from primary cultures of human SMCs. Currently, only one PDE1B gene has been reported, and no alternative ...
12
Transient hypercapnia reveals an underlying cerebrovascular pathology in a murine model for HIV-1 associated neuroinflammation: role of NO-cGMP signaling and normalization by inhibition of cyclic nucleotide phosphodiesterase-5
... 5'-nucleotidase activity) was added to each reaction, and reactions were incubated at 30°C for 10 ...Enzymatic activity was calculated as percentage total radioactivity minus back- ground, and was ...
14
cGMP stimulated cyclic nucleotide phosphodiesterase regulates the basal calcium current in human atrial myocytes
... PDE2 activity have also been ob- tained in a number of noncardiac cells, such as thymocytes (19), vascular endothelial (20) and smooth muscle cells (21), and PC12 cells ...
10
Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity
... and cyclic nucleotide balance within specific subcellular microdomains ...the activity of PDE2A towards the hydrolysis of cAMP is relatively low compared with cGMP (28), although it still contributes ...
17
Cyclic nucleotide metabolism during the development of murine thymic lymphoma
... cyclase activity was unaltered except when expressed 'per unit DNA' where there was a 50% decrease in tumour ...The phosphodiesterase activities, expressed 'per unit RNA or wet weight', were elevated in ...
495
The role of cyclic nucleotides and cyclic nucleotide phosphodiesterase in development of Mysococcus santus analysis of mutants.
... guanylate cyclase activities exhibited -peaks which would be consistent with their postulated roles. Devi and McCurdy (1984b) were also able to isolate two cAM P binding proteins and one eOMP binding protein.. OF the ...
59
Dual implication of 2',3' cyclic nucleotide 3' phosphodiesterase as major autoantigen and C3 complement binding protein in the pathogenesis of multiple sclerosis
... CNPase activity in CSF and serum in normal patients as well as patients with various neu- rological diseases which may originate from the OL/myelin membrane or lymphocytes (11, ...
10
Cyclic nucleotide phosphodiesterase 3A–deficient mice as a model of female infertility
... PKA activity per se is not critical, but rather it is the temporal and spatial dis- tribution and localization of cAMP signals and/or signaling path- ways and modules (37, 38, 44, 45) that organize and orchestrate ...
11
Alterations in regulation of energy homeostasis in cyclic nucleotide phosphodiesterase 3B–null mice
... Characteristics of Pde3b-KO mice. Newborn KO pups exhibited no obvious physical defects. Their growth and development, general behavior, and activity levels were similar to those of WT mice, and KO mice of both ...
13
Atrial natriuretic factor reduces cyclic adenosine monophosphate content of human fibroblasts by enhancing phosphodiesterase activity
... cellular cyclic guanosine monophosphate (cGMP) content and suppressed isoproterenol- and PGE1-elevated, but not basal, cAMP ...guanine nucleotide- binding protein that couples inhibitory receptors to ...
7
cAMP/PKA/CREB/GLT1 signaling involved in the antidepressant-like effects of phosphodiesterase 4D inhibitor (GEBR-7b) in rats
... Taken together, the results of this study suggest that GEBR-7b can reverse the downregulation of cAMP/ PKA/pCREB/GLT1 signaling under the chronic stress con- dition and improve depression-like behaviors of rats (The ...
9
Unique reporter-based sensor platforms to monitor signalling in cells
... CREB activity at concentrations up to 500 mM, above which CREB activity dropped significantly showing marked toxicity (Figure ...GRE activity was induced comparably by the addition of between ...of ...
10
Hyperglycemia raises the threshold of levosimendan- but not milrinone-induced postconditioning in rat hearts
... cardiomyocyte level, intracellular calcium overload occurs during post-ischemic reperfusion, and could cause cardiac arrhythmias or myocardial stunning. PDE- I causes the activation of cyclic adenosine monopho- ...
10
Changes of activity and isoforms of alkaline sphingomyelinase (nucleotide pyrophosphatase phosphodiesterase 7) in bile from patients undergoing endoscopic retrograde cholangiopancreatography
... NPP7 has 5N-glycosylation sites and defect glycosyla- tion reduces the enzyme activity [37]. Since the identifica- tion of the 1.4 and 1.2 kb products in the present study was based on Western blot, one might ask ...
7
Rieder, Florian (2004): Wirkung der spezifischen Phosphodiesterase-Inhibitoren Roflumilast und Pumafentrine im Mausmodell der Dextran-Sodium-Sulfat-induzierten Kolitis. Dissertation, LMU München: Medizinische Fakultät
... Emesis induced by inhibitors of type IV cyclic nucleotide phosphodiesterase. (PDE IV) in the ferret[r] ...
121