Cyclin-dependent Kinase 7
Cyclin-Dependent Kinase Inhibitor AT7519 as a Potential Drug for MYCN-Dependent Neuroblastoma.
... Given that Th-MYCN transgenic mice develop tumours in an immunocompetent background, we reasoned that the preclincal evaluation of AT7519 in this mouse model would be complementary to our xenograft data. Consistent with ...
22
Penta O galloyl β D glucose induces G1arrest and DNA replicative S phase arrest independently of P21 cyclin dependent kinase inhibitor 1A, P27 cyclin dependent kinase inhibitor 1B and P53 in human breast cancer cells and is orally active against triple negative xenograft growth
... (Figures 3 to 5). The clear disengagement of P53-Ser 15 phosphorylation from the best-known P53 transcrip- tional target P21 Cip1 in PGG-exposed MCF-7 cells remains an interesting question for further ...
11
Cyclin-dependent kinase 2 is an ideal target for ovary tumors with elevated cyclin E1 expression
... Consistent with published immunohistochemistry studies [7, 9, 24], we showed that over 40% of primary ovary tumor specimens were positive for CCNE1 staining (Table 1 and Figure 2). Although CCNE1 staining was ...
12
Retroviral Cyclin Enhances Cyclin-Dependent Kinase-8 Activity
... of kinase assay blots with specific antibodies did not detect CDK8 in anti-CDK7 or anti-CDK9 IPs, and there was no detectable CDK9 in anti-CDK8 or CDK7 IPs, but small quan- tities of CDK7 were detectable by the ...
10
Optimization of luminescent assay for screening of cyclin-dependent kinase 2 inhibitors
... Reactions were performed with reaction mixture containing serially diluted CDK2/cyclin A (0.976, 1.953, 3.906, 7.812, 15.62, 31.25, 62.5, 125, 250, 500 ng/well), histone H1 (10 mg/ml), 100 μM ATP, ADB (5 μl), 25 ...
5
Regulation of the G1-S transition by cyclin-dependent kinase inhibitors
... Human and murine cells senesce when grown in culture, but the dominant mechanism of growth arrest appears to be different. In many human (1, 10, 11, 19, 22, 47, 69, 71, 73) and some murine (70, 81) cell types, expression ...
103
Modulation of apoptosis by the cyclin dependent kinase inhibitor p27Kip1
... permits cyclin-dependent kinase 2 (CDK2) activity to be generated, allowing the cell to enter the S phase, followed by completion of the cell cycle ...
9
MicroRNAs in systemic rheumatic diseases
... CDK2, cyclin-dependent kinase 2; EGFL7, epidermal growth factor-like domain 7; IFN, interferon; IL, interleukin; IRAK1, IL-1 receptor- associated kinase 1; MCP-1, monocyte ...
10
Cip/Kip cyclin-dependent protein kinase inhibitors and the road to polyploidy
... of cyclin B1 expression [69,70], suppression of CDC25C expression [71] [the protein phosphatase that converts Cdk1-P into its active form ...CDK1:mitotic cyclin activity is needed to transit prophase, ...
15
Cyclin-dependent kinase 4 is a preclinical target for diet-induced obesity
... having no effect on lean mass (Figure 6). Although intermittent differences in food intake after abemaciclib treatment were observed, these were transient events that could not be continuously observed on a daily basis ...
16
Retroviral Cyclin Controls Cyclin-Dependent Kinase 8-Mediated Transcription Elongation and Reinitiation
... exogenous cyclin C. Ob- viously, increased levels of a CDK8-activating cyclin, whether RV- cyclin or cyclin C, can increase the output of IEG ...RV- cyclin expression will always be ...
12
Transcriptional Up-Regulation of the Cyclin D2 Gene and Acquisition of New Cyclin-Dependent Kinase Partners in Human T-Cell Leukemia Virus Type 1-Infected Cells
... Immunoprecipitation and immunoblotting. Cells grown in culture were spun at 10,000 ⫻ g for 15 min. The supernatants were discarded, and the pellets were washed twice with 25 ml of PBS without calcium or magnesium. The ...
11
Pharmacokinetics and biodistribution of the cyclin-dependent kinase inhibitor -CR8- in mice
... about 40%. Moreover, the cytotoxicity of CR8 against leukemic cell lines has been reported [7]. Altogether, these results suggest the possible use of CR8 in treating leukemia and lymphomas. However, the high ...
9
Cyclin dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis
... Calreticulin expression is regulated by a pathway that includes CDKN2B, retinoblastoma, and E2F4. To investigate the molecular link between CDKN2B and CALR, we next examined the CALR promoter for putative transcription ...
16
Functional Interactions between Herpesvirus Oncoprotein MEQ and Cell Cycle Regulator CDK2
... MEQ during the transformation process (40, 58). Given the strong homology of the bZIP domain of MEQ to the Jun/Fos family of transcription factors and its ability to form dimers with Jun and Fos, it is tempting to ...
12
The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy
... One kinase which when depleted by RNAi caused a particularly marked increase in GFP-LC3 puncta, was the cyclin-dependent kinase PITSLRE ...
7
Indirect p53-dependent transcriptional repression of Survivin CDC25C , and PLK1 genes requires the cyclin-dependent kinase inhibitor p21/CDKN1A and CDE/CHR promoter sites binding the DREAM complex
... Polo-like kinase family with five paralogs in vertebrates ...Ser/Thr kinase domain [13, ...Aurora kinase A, which requires Bora as a co-factor [20-22], and dephosphorylation at Thr210 by the ...
16
Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells
... In the present study, we demonstrated that an exogenous synthetic dsP53-285, which is completely complementary to sequences at positions − 285/ − 267 relative to the transcription start site of the p53 gene promoter, had ...
11
In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF 7 cell
... More recently, disease-oriented screening of medicinal plants is utilized as essential tool for new drug discov- ery. Medicinal plants derived from these studies are being increasingly used by the general public as ...
10
Ciclopirox activates ATR-Chk1 signaling pathway leading to Cdc25A protein degradation
... Ciclopirox olamine (CPX), an off-patent anti-fungal drug, has been found to inhibit the G 1 -cyclin dependent kinases partly by increasing the phosphorylation and degradation of Cdc25A. However, little is ...
14