Cyclin T1
Cyclin T1/CDK9 Interacts with Influenza A Virus Polymerase and Facilitates Its Association with Cellular RNA Polymerase II
... since cyclin T1/CDK9 knock- down only slightly reduced vRNP binding with total Pol II ...of cyclin T1/CDK9 in recruiting vRNP onto active transcribing Pol ...and cyclin H, was recruited ...
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Induction of TAK (Cyclin T1/P-TEFb) in Purified Resting CD4+ T Lymphocytes by Combination of Cytokines
... Combinations of cytokines are known to reactivate transcription and replication of latent human immuno- deficiency virus type 1 (HIV-1) proviruses in resting CD4 ⴙ T lymphocytes isolated from infected individuals. ...
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Induction of the HIV 1 Tat co factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs
... of cyclin T1 during MM6 differentiation, a siRNA-based strategy was used to knock down cyclin T1 ...of cyclin T1 mRNA, while transfected siRNAs typically induce only a transient ...
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A Cyclin T1 point mutation that abolishes positive transcription elongation factor (P TEFb) binding to Hexim1 and HIV tat
... wild-type Cyclin T1 (+++), or just showed growth impairment (+) and were not further investigated (Figure ...61 Cyclin T1 mutants did not grow at all (−) thereby confirming a loss in Hexim1 ...
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The Ability of Positive Transcription Elongation Factor b To Transactivate Human Immunodeficiency Virus Transcription Depends on a Functional Kinase Domain, Cyclin T1, and Tat
... Two cyclin-dependent serine/threonine kinases, CDK7 and CDK9, which phosphorylate the C-terminal domain of RNA polymerase II, have been implicated in Tat transactivation in vivo and in ...and cyclin ...
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A Minimal Chimera of Human Cyclin T1 and Tat Binds TAR and Activates Human Immunodeficiency Virus Transcription in Murine Cells
... The viral transcriptional transactivator Tat binds the trans- activation response (TAR) element RNA stem-loop to activate the expression of genes from and replication of human immu- nodeficiency virus type 1 (HIV-1) (16, ...
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Transient Induction of Cyclin T1 during Human Macrophage Differentiation Regulates Human Immunodeficiency Virus Type 1 Tat Transactivation Function
... The human immunodeficiency virus type 1 (HIV-1) Tat protein is essential for viral replication and stimulates transcription of the integrated provirus by recruiting the kinase complex TAK/P-TEFb, composed of ...
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Cyclin T1 and CDK9 T-Loop Phosphorylation Are Downregulated during Establishment of HIV-1 Latency in Primary Resting Memory CD4+ T Cells
... vitro, Cyclin T1 protein levels decrease as the result of proteasome-me- diated proteolysis (35); IL-10 accelerates this proteolysis of cyclin T1 in macrophages ...off Cyclin T1 ...
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Effects of prostratin on Cyclin T1/P TEFb function and the gene expression profile in primary resting CD4+T cells
... We also examined HEXIM1 protein expression levels and its association with CDK9. HEXIM1 was readily detectable in control cells and prostratin treatment induced its expression approximately two-fold in both donors (Fig. ...
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Optimized Chimeras between Kinase-Inactive Mutant Cdk9 and Truncated Cyclin T1 Proteins Efficiently Inhibit Tat Transactivation and Human Immunodeficiency Virus Gene Expression
... the cyclin-dependent kinase 9 (Cdk9) and cyclin T1, T2a, T2b, or K ...only cyclin T1 (CycT1) can support Tat transactivation (37, ...
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A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription
... the cyclin box (pale yellow) and in distance from the AFF4 binding surface and the binding region of CycT1 to Cdk9 (grey) ...b) Cyclin T1 mutations were positioned on the N-terminal helices in the ...
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Interactions between Equine Cyclin T1, Tat, and TAR Are Disrupted by a Leucine-to-Valine Substitution Found in Human Cyclin T1
... opposite side of the kinase-binding surface. This model of eT demonstrates that the leucine at position 29 and cysteine at position 261 are in close proximity (Fig. 6). The cysteine is required for the interaction ...
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Regulation of Cyclin T1 and HIV-1 Replication by MicroRNAs in Resting CD4+ T Lymphocytes
... against cyclin T1 or a negative-control siRNA in addition to miR-27b or a negative-control miRNA on day 0 (for immunoblotting demonstrating the efficacy of cyclin T1 siRNA ...
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Limited redundancy in genes regulated by Cyclin T2 and Cyclin T1
... containing Cyclin T1 has been exam- ined in detail because it is an essential host factor in HIV-1 gene ...However, Cyclin T2 has been reported to play a role in myocyte differentiation ...either ...
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Mice transgenic for equine cyclin T1 and ELR1 are susceptible to equine infectious anemia virus infection
... Therefore, humanized mouse models, such as severe combined immunodeficient (SCID) mice implanted with human fetal thymus and liver [13] or Rag22/2cc 2/2 mice injected with human hematopoietic stem cells (hHSCs) [14], ...
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Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells
... which Cyclin T1 (CycT1) is one of the most essential for viral RNA transcription in CD4 T cells and ...the cyclin-dependent kinase CDK9 to form the Positive Transcription Elongation Factor b ...
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Dominant negative mutant Cyclin T1 proteins inhibit HIV transcription by specifically degrading Tat
... the cyclin proteins CycT1, T2 and K revealed ten very well-conserved regions that are essential for the formation of the alpha-helical cyclin box repeat ...
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Identification of novel CDK9 and Cyclin T1 associated protein complexes (CCAPs) whose siRNA depletion enhances HIV 1 Tat function
... a Cyclin subunit, either CCNT1 or CCNT2 ...or Cyclin subunit may have distinct functional properties, as well as distinct expression patterns in differ- ent ...
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Tat-Associated Kinase, TAK, Activity Is Regulated by Distinct Mechanisms in Peripheral Blood Lymphocytes and Promonocytic Cell Lines
... Although cyclin T1 was difficult to detect in uninduced cells, the level of cyclin T1 appeared unchanged in both control and PMA-treated cells during the 3-h chase period ...of cyclin ...
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Antiapoptotic Function of Cdk9 (TAK/P-TEFb) in U937 Promonocytic Cells
... subunit, cyclin T1 (cycT1) as a regulatory sub- unit, and possibly other subunits that remain to be identified (30, 39, 42, ...regulatory cyclin subunit: cyclins T1, T2a, T2b, and possibly ...
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