Cytochrome b5 reductase
NADH cytochrome b5 reductase activity in lymphoid cell lines Expression of the defect in epstein Barr virus transformed lymphoblastoid cell lines from patients with recessive congenital methemoglobinemia
... Recessive congenital methemoglobinemia (RCM) is due to the homozygous deficiency of NADH-cytochrome b5 reductase (EC 1.6.2.2.). In type I disease, in which the patients are only methemoglobinemic, ...
8
Concentration of NADH cytochrome b5 reductase in erythrocytes of normal and methemoglobinemic individuals measured with a quantitative radioimmunoblotting assay
... erythrocyte reductase concentrations with a quantitative radioimmunoblotting method, using affinity-purified polyclonal antibodies against rat liver microsomal reductase as ...erythrocyte reductase ...
8
Membrane bound cytochrome b5 reductase (methemoglobin reductase) in human erythrocytes Study in normal and methemoglobinemic subjects
... methemoglobin reductase-like activity is unmasked by Triton X- 100 treatment, and represents about half of the total activity in the ...microsomal-bound cytochrome b5 reductase, the ...
8
Estimation of Cytochrome B5 Reductase Activity in Red Blood Cells of Rattus Norvegicus after the Treatment of Trichloroacetic Acid
... Experimental groups: The rats were divided into 5 groups (5 in each group). Group I as a control and the remaining groups (II-V) were the treated groups receiving 0.2% of TCA orally daily for the 60 days in drinking ...
5
Biochemical and anatomical basis of brain dysfunctions caused by cytochrome b5 reductase deficiency or dysregulation
... A: Biochemical functions of the Cb 5 R/Cb 5 redox system at cellular level in neurons: (1) Endoplasmic reticulum: Fatty acids desaturation - Steroids and xenobiotics metabolism - Sialic acid metabolism - Modulation of ...
5
Analysis of cytochrome b5 reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence
... In order to assess whether ZtCBR1 ablation had impacted on fatty acid biosynthesis by fungal cells, the fatty acid methyl ester (FAME) profile of the WT strain and D ZtCBR1-1 were analyse[r] ...
17
Cytochrome b5 reductase 2 suppresses tumor formation in nasopharyngeal carcinoma by attenuating angiogenesis
... PCR assays to evaluate changes in the mRNA levels of 84 genes related to cell proliferation, apoptosis, cell cycle, angiogenesis, invasion, and metastasis in HONE1 cells that were tra[r] ...
9
Congenital Methemoglobinemia: A Rare Cause of Cyanosis in the Newborn—A Case Report
... cell enzyme activity and DNA analysis revealed a ho- mozygous form of the cytochrome b5 reductase enzyme deficiency. He responded very well to daily methylene blue and ascorbic acid administration, ...
6
Journal of Applied Pharmaceutical Science
... by cytochrome P450 isozymes through oxidative pathways, reactive oxygen species generated in the presence of cytochrome b5 reductase may contribute to the carcinogenic effects of heterocyclic ...
7
Update of the NAD(P)H:quinone oxidoreductase (NQO) gene family
... A search of the HUGO Gene Nomenclature Committee database using ‘NQO’ provided three hits—two NQO genes plus ‘NQO3A2’ (which actually encodes cytochrome b5 reductase-1)—the latter appears in the ...
7
Cytochrome b5 Coexpression Increases Tetrahymena thermophila Δ6 Fatty Acid Desaturase Activity in Saccharomyces cerevisiae
... F atty acid desaturases are enzymes that introduce double bonds into fatty acid hydrocarbon chains at specific locations; the actual substrate of the reactions are fatty acids esterified to a car- rier molecule such as ...
9
Biochemical and biophysical characterisation of Anopheles gambiae NADPH-cytochrome P450 reductase
... fly reductase is able to oxidise two molecules of NADPH, but because NADPH will only readily bind to fully oxidised CPR, only oxidation of the first molecule is rapid enough to be considered part of the catalytic ...
282
62Cu Diacetyl Bis (N4 Methylthiosemicarbazone) PET in Human Gliomas: Comparative Study with [18F]Fluorodeoxyglucose and L Methyl [11C]Methionine PET
... Under hypoxic conditions, 62Cu-ATSM retention is induced by microsomal cytochrome reductase enzymes, which is different from [18F]fluoromisonidazole retention by mitochondrial nitroreduc[r] ...
7
Bile acid synthesis during development Mitochondrial 12 alpha hydroxylation in human fetal liver
... of cytochrome P450 showed 12 alpha-hydroxylase activity when combined with adrenodoxin and adrenodoxin reductase from bovine adrenal ...adrenodoxin reductase are components specific for mitochondrial ...
5
Mitochondrial complex I deficiency leads to increased production of superoxide radicals and induction of superoxide dismutase
... Mitochondria were isolated from skin fibroblast cultures de- rived from healthy individuals (controls) and from a group patients with complex I (NADH-CoQ reductase) deficiency of the mitochondrial respiratory ...
8
The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome c reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia
... NAD(P)H cytochrome c reductase decreased to 50% in PMN, no change was noted in ...NADPH cytochrome c reductases of the 16,000-g pellet decreased to 66+/-5%, 72+/-4%, 52+/-8%, and 40+/-9%, ...
12
Isolation, characterization and induction studies of human cytochromes P450
... A vast array o f P450 genes exist, 481 genes and 22 putative pseudogenes have been identified in 85 eukaryotes (including vertebrates, invertebrates, fungi and plants) and 20 prokaryote species (Nelson et a i, 1996). ...
369
Method for Detecting NADPH Cytochrome P450 Reductase in Liver Microsomal Fractions by Using Native Polyacrylamide Gel Electrophoresis and NADPH Diaphorase Staining
... P450 reductase in the stained gel was removed and dialysed in PBS at 4˚C overnight in a firm dialysis bag (molecular weight cut-off, 25 kD; Spectra/Por, Spectrum Japan, Tokyo, ...
5
Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli
... Previously, it has been shown that human CYP3A4 can be displayed in an active form on the surface of E. coli using the AIDA-I autotransporter [27]. The obtained whole cell biocatalyst was able to convert testosterone ...
15
Allelic Variation of Cytochrome P450s Drives Resistance to Bednet Insecticides in a Major Malaria Vector
... two cytochrome P450 genes is the most important driver of pyrethroid resistance in the major African malaria vector Anopheles funestus and detected key mutations controlling this ...
25