cytomegalovirus DNA
Comparison of Quantitative and Qualitative PCR Assays for Cytomegalovirus DNA in Plasma
... viral DNA is quantified by coamplifying a region of the CMV DNA polymerase gene in the presence of a known amount of quantitation ...CMV DNA copies of CMV DNA per milliliter of ...
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Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched DNA signal amplification assay
... CMV DNA by the bDNA assay, thereby yielding a 95% detection ...CMV DNA in these specimens may have been below the detection limit of the bDNA ...CMV DNA by the bDNA ...
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Molecular cloning and physical mapping of murine cytomegalovirus DNA.
... The results obtained after hybridization of 32P-labeled cloned fragments to Southern blots of MCMV DNA cleaved with HindIII, XbaI, EcoRI, BamHI, ApaI, ClaI, EcoRV, or KpnI allowed us to [r] ...
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Requirement for Uracil-DNA Glycosylase during the Transition to Late-Phase Cytomegalovirus DNA Replication
... into DNA arises through misincorpo- ration of dUTP by DNA polymerase (2, 51, 54) or from spon- taneous deamination of cytosine creating U:G base pair mis- matches that resolve into A:T transition mutations ...
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Multiple transforming regions of human cytomegalovirus DNA.
... Transforming activity of HCMV DNA fragments in NIH 3T3 cells DNA source Restriction endonuclease Towne XbaI-AO XbaI Towne XbaI-O XbaI Towne XbaI-C XbaI Towne XbaI-E XbaI Towne XbaI-E Xba[r] ...
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Relationship between Cytomegalovirus DNA Load in Epithelial Lining Fluid and Plasma of Lung Transplant Recipients and Analysis of Coinfection with Epstein Barr Virus and Human Herpesvirus 6 in the Lung Compartment
... template DNA extracted using a QIAamp viral RNA kit (QIAGEN, Hilden, Germany), 25 l of TaqMan universal PCR master mix (PE Applied Biosystems, Foster City, CA), 25 pmol of each primer, and 10 pmol of each ...
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Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis.
... Percentage of nuclei that incorporated [3H]TdR into cellular DNA and exhibited CMVspecific fluorescence after infection" % Nuclei incorporating [3H]TdR in: CMV-infected Mock-infected Pre[r] ...
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Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections
... HCMV DNA findings in 27 healthy blood donors and in the initial serum samples from 35 patients with serologically verified HCMV infection and clinical symptoms Group.. of HCMV DNA-positi[r] ...
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Characterization of guinea pig cytomegalovirus DNA.
... When HCMV HindIII fragments obtained by restriction endonuclease cleavage of DNA purified from HCMV AD169 were immobilized on nitrocellulose paper by the method of Southern 59 and 32P-la[r] ...
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Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay
... Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised pati[r] ...
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Human cytomegalovirus. IV. Specific inhibition of virus-induced DNA polymerase activity and viral DNA replication by phosphonoacetic acid.
... Specific Inhibition of Virus-Induced DNA Polymerase Activity and Viral DNA Replication by Phosphonoacetic Acid Phosphonoacetic acid specifically inhibited human cytomegalovirus DNA synth[r] ...
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Physical Requirements and Functional Consequences of Complex Formation between the Cytomegalovirus IE1 Protein and Human STAT2
... chloramphenicol resistance gene. For allelic exchange via conjugation, the recA ⫹ E. coli strain GS500 (66) containing pTNIE1kanlacZ and E. coli S17pir trans- formed with the pGS284-MIEdlIE1AD1-S/P donor plasmid were ...
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Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogens
... Complementary DNA; CDPH, California Department of Public Health; CLIA, Clinical Laboratory Improvement Amendments; CMV, Cytomegalovirus; CRISPR, Clustered regularly interspaced short palindromic repeats; ...
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Cytomegalovirus in urine: detection of viral DNA by sandwich hybridization
... The concentration procedure was as described in Table 2, experiment 2, except for 0.27 ml of urine from patient 1, which was tested unconcentrated, and 8 ml of urine from patient 3, whic[r] ...
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Human Cytomegalovirus I. Purification and Characterization of Viral DNA
... If we started with crude free virus with a relatively high viral particle number 1 5 x 10" particles per ml rather than virus extracted from cells, relatively pure CMV could be rather ea[r] ...
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Rapid detection of cytomegalovirus in clinical specimens by using biotinylated DNA probes and analysis of cross reactivity with herpes simplex virus
... Rapid Detection of Cytomegalovirus in Clinical Specimens by Using Biotinylated DNA Probes and Analysis of Cross-Reactivity with Herpes Simplex Virus NELL S.. FARRAND't Departments of Mic[r] ...
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Comparison of Three Assays for Cytomegalovirus Detection in AIDS Patients at Risk for Retinitis
... and/or DNA load are present may delay the de- velopment of retinitis (1, 5, ...CMV DNA System, and (iii) qualitative plasma CMV DNA detection with the Roche Amplicor Qual- itative PCR ...
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Real Time Automated PCR for Early Diagnosis and Monitoring of Cytomegalovirus Infection after Bone Marrow Transplantation
... We have established a new method of diagnosis of CMV infection using real-time automated PCR. This is a modifica- tion of conventional PCR, and it possesses the advantages of this assay. Although real-time automated PCR ...
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Human cytomegalovirus. III. Virus-induced DNA polymerase.
... Activated calf thymus DNA was used as the template primer for the host cell enzyme a and b, and polydA .oligodT,2 18 was used in virus-induced DNA polymerase assays c and d.. infection w[r] ...
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Quantification of DNA in Plasma by an Automated Real Time PCR Assay (Cytomegalovirus PCR Kit) for Surveillance of Active Cytomegalovirus Infection and Guidance of Preemptive Therapy for Allogeneic Hematopoietic Stem Cell Transplant Recipients
... no DNA level cutoffs for plasma specimens have been clinically ...CMV DNA in plasma or the occurrence of two consecutive positive PCR results, 18 and 16 episodes of self-resolving active CMV infections, ...
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