Cytotoxic T cell epitope
Use of recombinant protein to identify a motif-negative human cytotoxic T-cell epitope presented by HLA-A2 in the hepatitis C virus NS3 region.
... an epitope on the NS3 protein by examining targets infected with vaccinia virus containing this segment of the dengue virus ...CTL epitope in the NS3 region of HCV has recently been identified by using a ...
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Identification of the Immunodominant H-2Kk-Restricted Cytotoxic T-Cell Epitope in the Borna Disease Virus Nucleoprotein
... We mapped the CTL epitope in BDV p40 by two comple- mentary experimental approaches. In a first approach, we gen- erated C-terminally truncated versions of p40 that were sub- sequently introduced into recombinant ...
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Definition of a minimal optimal cytotoxic T-cell epitope within the hepatitis B virus nucleocapsid protein.
... The aim of the present study was to identify the minimal optimal sequence recognized by HBcAg1ll27-specific CTLs in order to more thoroughly characterize the CTL response to this epitope[r] ...
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Cytotoxic T-Lymphocyte Epitope Vaccination Protects against Human Metapneumovirus Infection and Disease in Mice
... a T-cell response to ...mononuclear cell infiltration and an inflammatory environment conducive to CTL response ...of T cells or NK cells results in increased viral titers in the lungs of ...
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Distinct HIV-1 Escape Patterns Selected by Cytotoxic T Cells with Identical Epitope Specificity
... The present study demonstrated that HLA-B*52:01-restricted, Pol283-8-specific CTLs also had a strong ability to suppress HIV-1 replication in vitro (80% suppression at an E/T cell ratio of 0.3:1). However, ...
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Fitness Costs Limit Viral Escape from Cytotoxic T Lymphocytes at a Structurally Constrained Epitope
... the cell supernatants were collected and the cells were lysed for 20 min with a solution containing 50 mM Tris-HCl (pH ...The cell lysates were centrifuged for 20 min at 16,000 ⫻ g, and the soluble fraction ...
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The generation of cytotoxic T cell epitopes and their generation for cancer immunotherapy
... Vanaf 1994, mijn start in de tumorimmunologiegroep, is er veel veranderd binnen de groep en binnen de afdeling IHB. Zelfs het meubilair is tenminste twee keer vernieuwd. Desalniettemin zijn er een aantal vaste waarden ...
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T-cell receptors from virus-specific cytotoxic T lymphocytes recognizing a single immunodominant nine-amino-acid viral epitope show marked diversity.
... Cytoplasmic RNA was extracted from CTL clones by using Nonidet P-40-sodium dodecyl 352 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Following infection of the H-2d mo[r] ...
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Common Antiviral Cytotoxic T-Lymphocyte Epitope for Diverse Arenaviruses
... whereas T lymphocytes interact with viral antigens in the form of pro- cessed peptides bound to host cells and presented in a groove between the two ␣-helices of the major histocompatibility complex (MHC) ...
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Identification of a Naturally Processed Cytotoxic CD8 T-Cell Epitope of Coxsackievirus B4, Presented by HLA-A2.1 and Located in the PEVKEK Region of the P2C Nonstructural Protein
... viral epitope from whole protein and/or whole virus to CD8 T cells, DCs were generated as de- scribed above from the same source as the CD8 T-cell line (autologous; HLA- ...uninfected ...
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Cytotoxic T-Lymphocyte Epitope Immunodominance in the Control of Choroid Plexus Tumors in Simian Virus 40 Large T Antigen Transgenic Mice
... established cell lines from these tumors, have greatly reduced MHC class I cell surface expression which might con- tribute to tumor escape from SV40 Tag-specific ...transformed cell lines undergoes ...
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Identification of a T-helper cell epitope on the rotavirus VP6 protein.
... that are relevant for protection. Moreover, particular attention must be paid to heterotypic responses, since in humans there are at least four different epidemiologically important rota- virus G serotypes (35). The ...
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Target epitope in the Tax protein of human T-cell leukemia virus type I recognized by class I major histocompatibility complex-restricted cytotoxic T cells.
... 2928-2933 0022-538X/92/052928-06$02.00/0 Copyright © 1992, American Society for Microbiology Target Epitope in the Tax Protein of Human T-Cell Leukemia Virus Type I Recognized by Class I[r] ...
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Functional management of an antiviral cytotoxic T-cell response.
... FIG. 1. Fine specificity of a monoclonal versus polyclonal LCMV-specific CTL response. (A) Amino acid sequences of peptides (pep) derived from peptide p33, which defines the major CTL epitope of LCMV. The ...
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Effects of Cytotoxic T Lymphocytes (CTL) Directed against a Single Simian Immunodeficiency Virus (SIV) Gag CTL Epitope on the Course of SIVmac239 Infection
... SIV RNA from plasma were measured by using Chiron’s SIVmac branched DNA RNA assay (solid lines). Levels of Gag CM9-specific T-cell responses, as measured with tetramers, are also shown (bars). Time points ...
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The Simian Immunodeficiency Virus Envelope Glycoprotein Contains Two Epitopes Presented by the Mamu-A*01 Class I Molecule
... Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with ...target cell ...
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Cross-Reactivity of T Lymphocytes Recognizing a Human Cytotoxic T-Lymphocyte Epitope within BK and JC Virus VP1 Polypeptides
... HLA-A*02-restricted epitope within the VP1 polypeptide of a human polyomavirus, BK virus (BKV), which is associated with polyomavirus-associated nephropathy in kidney transplant ...of cytotoxic T ...
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Compensatory Mutations Restore the Replication Defects Caused by Cytotoxic T Lymphocyte Escape Mutations in Hepatitis C Virus Polymerase
... KSKKTPMGF epitope impair T cell ...KSKKTPMGF epitope impaired CD8 ⫹ T cell ...F-specific T cell line was derived from an individual chronically infected with ...
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Epitope-Dependent Avidity Thresholds for Cytotoxic T-Lymphocyte Clearance of Virus-Infected Cells
... natural epitope presentation) to detect the pres- ence of virus-specific CTLs by observing cytokine release or direct TCR binding, but ultimately, whether CTLs can recog- nize and kill an infected target ...
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Naturally Occurring TAP-Dependent Specific T-Cell Tolerance for a Variant of an Immunodominant Retroviral Cytotoxic T-Lymphocyte Epitope
... target cell surface presentation of RSPWFTTL in vitro by targeting to the ER with signal-sequence (ss)-tagged mini- gene constructs (Vac 13ss) ...RSPWFTTL epitope into the ER did allow for its cell ...
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