DCFH-DA
Journal of Applied Pharmaceutical Science
... Iso-caryophyllene, a sesquiterpene, is present in several essential oils from vegetable species. In previous work, iso-caryophyllene was found cytotoxic against in vitro culture cell lines but its mechanism of action is ...
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Protective effects of γ-aminobutyric acid against H2O2-induced oxidative stress in RIN-m5F pancreatic cells
... Viable cells were harvested, and dichloro-dihydro-fluorescein diacetate (DCFH-DA) was used to detect reactive oxygen species (ROS) level; cellular redox status and insulin secretion were[r] ...
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Meripilus giganteus ethanolic extract exhibits pro apoptotic and anti proliferative effects in leukemic cell lines
... After 24 h treatment Jurkat and HL-60 cells (1.00 × 10 6 cells/mL), were washed twice in HBSS, and incubated with 5 μM 2′-7′-dichlorodihydrofluorescin diacetate (DCFH-DA) (Sigma-Aldrich, Saint Louis, MO, ...
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Metal-based NanoEnhancers for Future Radiotherapy: Radiosensitizing and Synergistic Effects on Tumor Cells
... cells; DCFH-DA: 2’,7’-dichlorofluorescein diacetate; DDR: DNA damage-response; DDS: drug delivery systems; DEF: radiation dose enhancement factor; DMPK: drug metabolism and pharmacokinetic; DMSO: dimethyl ...
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IN-VITRO NEPHROPROTECTIVE ROLE OF ETHANOLIC ROOT EXTRACT OF Boerhaavia diffusa AGAINST CISPLATIN-INDUCED NEPHROTOXICITY
... Graph 6: Effect of ERE on ROS induced by cisplatin. LLC-Pk1 was incubated with cisplatin with or without ERE for 24 hours. To measure the intracellular peroxide formation DCFH-DA was used as a probe. This ...
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Verbesina encelioides: cytotoxicity, cell cycle arrest, and oxidative DNA damage in human liver cancer (HepG2) cell line
... Abbreviations %: percentage; °C: degree celsius; A-549: human lung cancer cell line; CO2: carbon dioxide; DCFH-DA: dichloro-dihydro-fluorescein diacetate; DMEM: Dulbecco’s modified eagle[r] ...
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Conditioned media from adipose stromal cells limit lipopolysaccharide induced lung injury, endothelial hyperpermeability and apoptosis
... BALF from saline lungs, neutrophils were the dominant cell type in BALF from LPS-challenged lungs. Although ASC or ASC-CM treatment did not shift the overall neutro- phil/macrophage balance (Figure 5A,B), a significant ...
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Jingfukang induces anti cancer activity through oxidative stress mediated DNA damage in circulating human lung cancer cells
... Jinfukang oral liquid freeze-dried powder was prepared and detected the fingerprint (Additional file 2: Table S2) by Pro- fessor Yu Jin of East China University of Science and Tech- nology (Shanghai, China). A cell ...
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Red ginseng abrogates oxidative stress via mitochondria protection mediated by LKB1 AMPK pathway
... RGE was provided by Korea Tobacco & Ginseng Corporation (Daejeon, Korea) [22]. AA and compound C were purchased from Calbiochem (San Diego, CA). Anti-procaspase-3, anti- phospho-acetyl-CoA carboxylase (ACC), ...
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<p>In vitro mitochondrial-targeted antioxidant peptide induces apoptosis in cancer cells</p>
... Figure 3 The levels of intracellular and mitochondrial ROS in different cell lines after treatment with KRA and the KRSH peptide for 24 h were detected using DCFH-DA and MitoSOX TM.. , a[r] ...
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Intracellular free radical production by peripheral blood T lymphocytes from patients with systemic sclerosis: role of NADPH oxidase and ERK1/2
... M DCFH-DA for 20 minutes, and fluorescence was measured on a plate reader ...subjects. DCFH-DA, 2 ′ , 7 ′ -dichlorodihydrofluorescin diacetate; PBL, peripheral blood lymphocytes; ROS, reactive ...
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Original Article Emodin induces apoptosis of human osteosarcoma cells via mitochondria- and endoplasmic reticulum stress-related pathways
... Abstract: Aim: Emodin showed anti-cancer activity against multiple human malignant tumors by inducing apoptosis. However, the apoptotic inducing effect against human osteosarcoma and related mechanism are still not ...
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“Neuroprotective Effects of Rutin against Hydrogen Peroxide Induced Oxidative Stress in Cerebral Glioma Cell Line” by Gaurav Kumar, Jayanand, Quaisar Perween, Rihan Ansari, Durg Vijay Rai, India.
... probe DCFH- DA (2, 7- dichloro dihydrofluorescein diacetate). 17 DCFH- DA is a nonfluorescent compound, and it can be enzymatically converted to highly fluorescent compound, DCF, in the ...
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Reforma da Administração Pública: Antes e Depois da Democracia
... ou da organização político-representativa do Estado; reformas vindas ou provocadas do exterior ou por acção própria; com um efusivo entusiasmo inicial ou não; improvisada ou não; dentro ou fora do prazo que se ...
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Transtorno do espectro da esquizofrenia: um olhar da família
... torno da expectativa de cura. De maneira geral, o desgaste da família ao cuidar de uma pessoa com esquizofrenia deriva, em parte, pelo fato de que as redes de assistência não conseguem atingir essas ...
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Análise espacial da centralidade e da dispersão da riqueza gaúcha de 1970 a 2000: notas preliminares
... relativo da região de Caxias do ...médios da renda mensal per capita: entre 1970 e 1980, o deslocamento vai em direção ao nordeste do Estado; e, na década seguinte (1980-91), sofre uma inflexão em direção ...
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Comparison of fluorescence-based techniques for the quantification of particle-induced hydroxyl radicals
... only DCFH, APF, and amplex ultrared in a pH buffered water solution, the addition of H 2 O 2 & HRP results in much greater fluorescence ...of DCFH, APF, and amplex ...
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Neonatal Brachial Plexus Paralysis: A Discussion on the Light of Literature
... severidade da lesão, é importante distinguir dois tipos de lesão: a lesão pré-ganglionar e a ...acima da raiz ...planejamento da cirurgia. Já a lesão pós-ganglionar é localizada abaixo da raiz ...
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Da Pena Privativa de Liberdade: Controle da Crimilalidade?
... as da ilha das Cobras e Santa Bárbara, detinham escravos enviados para “correção” por seus donos, devido a uma falta cometida por eles, com fugir ou contrair uma doença ...sair da prisão sem o consentimento ...
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Representação do aleitamento materno para acadêmicos da área da saúde
... No decorrer das entrevistas, ficou evidente o quanto os acadêmicos enfantizam as vantagens oferecidas através do aleitamento materno. O ponto mais frisado foi sobre a fonte de nutrição, crescimento e desenvolvimento, ...
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